Carbon-based bio-organic fertilizer for improving degraded vegetable soil and application thereof
A bio-organic fertilizer and vegetable field technology, applied in the field of carbon-based bio-organic fertilizer, can solve the problems of inability to deeply improve the soil biological properties of degraded vegetable fields, single function of carbon-based organic fertilizer, unclear soil improvement effect, etc. Microflora and biological activity, the effect of promoting crop growth, and the effect of improving disease resistance and stress resistance
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Embodiment 1
[0042] Embodiment 1 Bacillus amyloliquefaciens BS211 potted disease prevention effect
[0043] Inoculate Bacillus amyloliquefaciens BS211 on a beef extract peptone agar plate and culture at 32°C for 2 days; then pick a single colony of BS211 strain on the plate and transfer it to beef extract peptone liquid medium with a volume of 200m1 / 500m1 , the rotating speed of the shaking table was 160rpm, and the shaking table was cultured at 32°C for 2 days to obtain the BS211 bacterial liquid. At this time, the concentration of the bacterial liquid was counted as 6.3×10 8 CFU / mL.
[0044] Inoculate Bacillus amyloliquefaciens L3 on a beef extract peptone agar plate and culture at 32°C for 2 days; then pick a single colony of L3 strain on the plate and transfer it to beef extract peptone liquid medium with a liquid volume of 200m1 / 500m1 , the rotation speed of the shaking table was 160rpm, and the shaking table was cultivated at 32°C for 2 days to obtain the L3 bacterial liquid. At thi...
Embodiment 2
[0047] Embodiment 2 Bacillus amyloliquefaciens L3 sand culture promoting effect
[0048] Get 20 mesh quartz sands, be divided into three groups, add and account for the quartz sands mass percent and be respectively 10% aseptic beef extract peptone culture solution (control group), 10% L3 bacterium liquid (experimental group 1) that embodiment 1 obtains, 10% of the L3 bacterium solution obtained in Example 1+10% of the BS211 bacterium solution obtained in Example 2 (experimental group 2), sow cucumber seeds again, and the real scene of sand culture after 7 days of sowing is as follows figure 2 shown.
[0049] The germination rate of the control group was 71.8%, and the average fresh weight was 2.81g / tree. The germination rate of experimental group 1 was 91.3%, and the average fresh weight was 3.92g / tree. The germination rate of experimental group 2 was 96.9%, and the average fresh weight was 4.52g / tree. It can be seen that L3 bacteria can significantly promote the growth an...
Embodiment 3
[0050] Embodiment 3 is rich in the organic carrier of BS211 and L3 bacillus amyloliquefaciens
[0051] a) Inoculate Bacillus amyloliquefaciens BS211 on a beef extract peptone agar plate, and culture at 32°C for 2 days; then transfer a single colony of BS211 strain picked on the plate to beef extract peptone liquid medium, and the liquid volume is 400m1 / 1000m1, cultivated on a shaking table at 32°C for 2 days, and the rotating speed of the shaking table was 160rpm; then transferred three parts of 400m1 culture solution to a 10L seed tank with an inoculum size of 12% (volume ratio), and cultivated at 32°C for 1 day, with an aeration rate of 0.8 L / L min; finally, the seed liquid was transferred to a 200 L fermenter, and cultured at 32°C for 2 days, with an aeration rate of 0.6 L / L min. The biomass of the BS211 bacterial solution out of the tank is 8.2×10 8 CFU / mL, sporulation rate was 94.7%.
[0052] b) Inoculate Bacillus amyloliquefaciens L3 on a beef extract peptone agar pla...
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