306 results about "Pediococcus acidilactici" patented technology

The invention relates to a preparation method of a biological preservative. As known to all, along with the rapid development of the society, chemical preservatives have been used in food, have strong impact on food safety and endanger the health of consumers, therefore, the use of preservatives causes great concern of government departments and consumers. According to the biological preservative, natural plants and microbial antibiotic substances are used as base materials, for example, the biological preservative is prepared by mixture of carboxymethyl chitosan, xylooligosaccharide, nisin from lactic streptococci, acetic acid, lactobacillus reuteri, pediococcus acidilactici, calcium citrate, ionized calcium, lactic acid, plant essential oil and water. The biological preservative can inhibit growth of harmful microorganisms by antagonistic and competitive inhibition effects among different microorganisms and produce extracted and separated components of antibacterial active ingredients having antifungal and antibacterial functions in normal growth and metabolism processes, thereby achieving antiseptic and fresh-keeping effects.

A dry, stable and viable probiotic composition comprising, a probiotic microorganism and a dried plant powder, with the proviso that said composition is not a blended mixture of at least one biologically pure Pediococcus acidilactici probiotic culture and dried tomato powder at a weight ratio of 1:4 encapsulated in an effective amount in a gelatin capsule.

The invention discloses a composite microbial agent and a preparation method and application thereof. The composite microbial agent includes a pseudomonas preparation, an acinetobacter preparation, a bacillus amyloliquefaciens preparation, a bacillus subtillis preparation, a bacillus licheniformis preparation, a pediococcus acidilactici preparation, a rhodobacter sphaeroides preparation, an alkali-lake dietzia preparation and a rhodopseudomonas palustris preparation. The preparation method includes the following steps that 1, composite microbial agent components are prepared; 2, the composite microbial agent components are mixed in proportion to obtain the composite microbial agent. The composite microbial agent can simultaneously decompose different organic matter, degrade amino nitrogen and nitro nitrogen in water, reduce phosphorus concentration in the water body and inhibit the growth of algae. The composite microbial agent is used for urban-rural sewage in-situ remediation, has the advantages of being high in efficiency, quick to take effect, free of no secondary pollution, simple in operation and the like, and large-scale application and popularization are facilitated.

The invention provides a composite microbial flora and an enzyme product thereof, and a method of applying the composite microbial flora and the enzyme product thereof in soil restoration. The composite microbial flora is composed of a microbial flora and a bioactive enzyme, wherein the microbial flora is composed of bacillus subtilis, bacillus mucilaginosus, bacillus amyloliquefaciens, pediococcus pentosaceus, pichia farinose and pediococcus acidilactici, and the bioactive enzyme is composed of phytase, protein hydrolase, cellulose hydrolase and urease. Compared with the prior art, an effective microbial community and a microecological environment are constructed to realize biological catalysis and improve the content of organic matters and beneficial microbial flora in soil, and thus, the fertility of the soil is improved; biochemical degradation rate of soil pollutant can be substantially accelerated, digestion and biodegradation of the pollutant can be accelerated, and final inertial products like carbon dioxide, water and nitrogen are obtained; and toxic intermediate produced in the process of biochemical degradation can be rapidly removed, odor is eliminated, and emission of organic volatile matters (VOC) is reduced.

The invention provides a biological treatment agent capable of efficiently degrading kitchen waste. The biological treatment agent capable of efficiently degrading kitchen waste comprises a microbial agent and a carrier, and the mass ratio of the microbial agent to the carrier is (1-3):(10-15). By the biological treatment agent capable of efficiently degrading the kitchen waste, brevibacterium flavum, bacillus licheniformis, bacillus subtilis, candida glabrata and pediococcus acidilactici have the synergistic effect on the carrier, the kitchen waste can be degraded efficiently, and decreased amount of fed kitchen waste within 24 hours can be 95% or above. The biological treatment agent is stable in treatment effect and low in cost; the preparation method is simple and convenient; environmental pollution does not exist.

The invention provides high-efficiency degradation nitrite pickled vegetable direct vat starter and a preparation method thereof. The high-efficiency degradation nitrite pickled vegetable direct vat starter comprises plantaricin, leuconostoc mesenteroides, pediococcus acidilactici and the freeze-dried fungus powder of lactobacillus brevis which are mixed by equal mass. The method prepares the high bacteria count starter under nutrition, growth and rich freeze-drying conditions by keeping bacterial strain with degradation nitrite through CICC, so the method has the advantages of product safety and no side effect. As determined, the bacterial count is equal to or more than 1010CFU / g. 150kg of Chinese cabbage is well fermented if fermented under 20 DEG C for 10 days; 150kg of Chinese cabbage is well fermented if fermented under 30 DEG C for 5 days; the mixed fermented degradation nitrite is equal to or more than 98 percent; the pickled vegetable has good flavor and excellent product quality. The invention can greatly shorten the vegetable fermentation time by 3 to 5 days, and can be used in industrialized production.

An exemplary embodiment providing one or more improvements includes feeding animals with probiotic microbes encapsulated in a mixture of xanthan gum and chitosan, or in gelatin, specifically Pediococcus acidilactici and Saccharomyces boulardii. Such encapsulation protects the viability of the probiotic microbes against unfavorable temperatures. An exemplary embodiment providing one or more improvements includes methods of using viable probiotics in therapy of birds and mammals infected with infectious diseases. Probiotics acted as adjuvants in stimulating antibody reaction and stimulated a cellular immunity response. In particular, probiotics were shown to reduce the number of viable oocytes from fecal samples, stimulate antibody production, and stimulate of proliferation of splenocytes in chickens infected with Elimeria. In addition, probiotics were shown to relieve symptoms of parvovirus infection in dogs.

This invention discloses Pediococcus acidilactici LH31 (CCTCC M207013), and a method for producing pediocin from it. The method comprises: inoculating Pediococcus acidilactici LH31 in modified MRS liquid medium, culturing, activating, inoculating activated Pediococcus acidilactici LH31 in a fermentation tank, and performing feed-batch fermentation with three stages. Pediococcus acidilactici LH31 is proliferated in stages 1 and 2. The maximal bacterial density (OD600 nm) can reach 30. Pediocin is accumulated in stages 2 and 3. The maximal titer can reach 18000 AU / mL. The method is suitable for industrialization.

The invention discloses a plant microecological preparation for enhancing the immunity of an animal and a preparation method of the plant microecological preparation, and belongs to the field of feed additives. The plant microecological preparation is prepared by the following steps: mixing crushed materials or extracted materials of radix pseudostellariae, liquorice, rhizoma atractylodis macrocephalae, honeysuckle, astragalus membranaceus, eucommia ulmoides, great burdock achene, dandelion and herba epimedii, adding a culture solution to prepare a culture medium, adding a total bacterium liquid consisting of two or more than two of lactobacillus plantarum, enterococcus faecalis, enterococcus faecium, enterococcus faecium, pediococcus acidilactici, pediococcus pentosaceus, rhodopseudomonas palustris, bacillus subtilis and lactobacillus fermenti, fermenting and drying. The plant microecological preparation disclosed by the invention has the advantages that the immunity of the animal can be obviously improved, the production performance can be improved and the diarrhea rate can be reduced.

The invention relates to a high-density culture method of lactic acid bacteria, which takes Lactobacillus plantarum, Lactobacillus bulgaricus and Pediococcus acidilactici as bacterial strains, a special lactic acid bacteria culture medium is used, the basic formula includes: 3 percent soybean peptone, 2 percent yeast extract, 0.5 percent sodium acetate, composite trace element liquid and composite vitamin solution, etc., the initial pH value is regulated to 7.0 to 7.2; Na2CO3, KOH, NaOH or ammonia is fed during the culture process to allow the pH value of a culture liquid to be maintained at 6.0 to 6.8. The inhibition of the growth of the self-metabolic product is eliminated, the high-activity and high-concentration lactic acid bacteria culture medium is obtained, and the living bacterial number of the lactic acid bacteria in the culture liquid can achieve 9.0 multiplied by 10<9> to 1.0 multiplied by 10 <10> cfu / ml.

The invention discloses a biological feed additive capable of effectively enhancing immunity of piglets, which belongs to the field of a feed additive. The additive comprises a compound Chinese herbal medicine and a micro-ecologic preparation, wherein the Chinese herbal medicine comprises pseudo-ginseng, dendrobe, cacumen biotae, eucommia ulmoides, rhodiola rosea, officinal magnolis flower, astragalus membranaceus, platycodon grandiflorum, orange peel, burdock, isatis root and sea-buckthorn; the micro-ecologic preparation is composed of bacillus subtilis, bacillus licheniformis, bifidobacterium, lactobacillus buchneri and pediococcus acidilactici. Due to the adoption of the feed additive, the growth performance of piglets can be improved, and the immunity of the piglets can be enhanced at the same time, and furthermore, the feed additive has a very wide market prospect.

There are provided pediocin-producing pediococci that have an enhanced survival property in the small intestine. The pediococci comprise in particular Pediococcus acidilactici isolated from human faeces. The pediococci are suitable for use in the gastrointestinal tract of humans to provide a health-promoting action, in particular against infection by multi-resistant pathogens. Also provided herein are compositions incorporating such pediocin-producing pediococci.

The invention relates to preparation and application of a diarrhoea-resistant type special composite microecological preparation for piggies, belongs to the field of a feed additive, solves the technical problems of the special composite microecological preparation prepared from various beneficial microorganisms and having favorable biology diarrhoea-resistant effects, for the piggies, the product strain compatibility and the feeding effect stability, and relates to the diarrhoea-resistant type special composite microecological preparation for piggies. The diarrhoea-resistant type special composite microecological preparation is characterized by being formed by combining bacterium powder I, with bacterium powder II and a carrier, wherein the bacterium powder I is coated composite lactic acid bacteria consisting of coated enterococcus faecalis and pediococcus acidilactici; the bacterium powder II is composite bacillus formed by mixed fermentation of three probiotics of bacillus subtilis, bacillus coagulans and clostridium butyricum. Various beneficial microorganisms in the diarrhoea-resistant type special composite microecological preparation for piggies have the characteristics of being high in bacterium restraining capacity, rapid in propagation, capable of realizing synergistic symbiosis, rich in produced enzymes and high in enzyme activity. A technique of united fermentation of various bacterium strains and strain compatibility is adopted for preparing the diarrhoea-resistant type special composite microecological preparation, so that the diarrhoea-resistant type special composite microecological preparation is worth of wide popularization and application.

The invention relates to a compound biological deodorizer as well as a preparation method and application thereof. The compound biological deodorizer comprises a plant extract, pichia pastoris, pediococcus pentosaceus, pediococcus acidilactici, candida mycoderma and bacillus megatherium. Through the utilization of compound biological deodorizer to treat a waste transfer station, a livestock and poultry farm and a composting site, the NH3 removal rate can respectively reach 65.2-71.6%, 63.4-73.7% and 53.42-75.77%, and the H2S removal rate can reach 53.5-60.1%, 35.5-45.9% and 47.2-53.8%, so that the odor concentration is obviously reduced, the environment of the periphery of the waste transfer station, the livestock and poultry farm and the composting site is effectively improved, and the improvement of both economic benefits and environment-friendly benefits is facilitated.

A novel representational system that is a method for improving feeding probiotics is provided. The probiotics, especially the Pediococcus acidilactici and the Saccharomyces boulardii are mixed in different colloid net including xanthan, chitosan or gelatin. By using said mixed colloid the activity of the probiotics can be protected, and multiple execrable surroundings and disadvantageous temperature for growth can be prevented. The novel representational method also provides many kinds of method for treating infectious disease for birds and mammals by using activated probiotics. Furthermore, the probiotics is usually acted as adjuvants for improving the generation of antibody and the cellularity immunity effect. In particular, probiotics were shown to reduce the number of viable oocytes from fecal samples, stimulate antibody production, and stimulate of proliferation of splenocytes in chickens infected with Elimeria. In addition, probiotics were shown to relieve symptoms of parvovirus infection in dogs.

The invention belongs to the technical field of agricultural microorganisms and aquatic product processing, and particularly relates to a compound starter for lactobacillus plantarum and application thereof, wherein the compound starter is used as a microbial starter, is especially suitable for freshwater fish fermentation, and is composed of lactobacillus, pediococcus acidilactici and staphylococcus xylosus. A lactic acid bacterium, namely lactobacillus plantarum, suitable for freshwater fish fermentation is obtained through self separation and screening, and the preservation number in the China center for typical culture collection is CCTCC NO: M2012396. The compound microbial starter is prepared from lactobacillus plantarum, pediococcus acidilactici and staphylococcus xylosus, is suitable for fish product fermentation and particularly suitable for the application to preserved fish fermentation, has a sound fermenting property, has typical fermentation fragrance, and facilitates industrial production.

The invention discloses newly separated pediococcus acidilactici HEW-AP27 collected in the CGMCC (China General Microbiological Culture Collection Center) in the Institute of microbiology of CAS (Chinese Academy of Sciences) in Beichen west road No.1 yard No.3 in Chaoyang District of Beijing with the postal code of 100101 on March 7, 2018, with the collection number of CGMCC NO.15419. The invention also provides a preparation method of a viable pediococcus acidilactici preparation. The method comprises the steps as follows: the pediococcus acidilactici is used as a fermentation strain, an optimized fermentation process and a fermentation medium are adopted, discharging is performed after first-stage, second-stage and third-stage culture, active bacterial sludge is obtained through centrifugation, a stabilization protective agent is added, granulation is performed through a granulator, a coating liquid is added to a coating machine for coating, and finally, the viable pediococcus acidilactici preparation is obtained. The pediococcus acidilactici HEW-AP27 has acid resistance, cholate resistance and high-temperature resistance, has high acid producing performance and can inhibit growth and reproduction of pathogenic bacteria.

The invention provides Pediococcus acidilactici (CGMCC No.2312) which is separated and filtrated and is suitable for fermentation of meat. The Pediococcus acidilactici is used as starter for the meat. Sausages with addition of the starter have special flavor, color and texture and are meat products which have long preservation period and can be directly ate without heating. The meat products of the invention are fermented, thereby unpleasant odor such as fishy smell, cowy flavor and so on of the meat can be removed; nitrite residual in the fermented meat products is greatly reduced, thereby the food safety is improved. Simultaneously, the meat is tenderized and the pH of the fermented meat products is low, thereby the growth of spoilage organisms and pathogen and the production of toxin are inhibited; the preservation period of the meat products is greatly prolonged; storage and circulation are convenient; the meat products are safe to eat; and the content of amino acids is high. Moreover, useful compositions are not damaged, thereby the meat products are rich in nutrition and easy in absorption.

The invention discloses a compound microbial agent for low-temperature fermentation of straws. The compound microbial agent comprises trichoderma viride, aspergillus niger, bacillus subtilis, lactococcus, pediococcus acidilactici and bacillus circulans. In order to prolonging normal-temperature storage time of the compound microbial agent, the compound microbial agent further comprises auxiliary materials including a bacterium preserving agent, a carrier, an antioxidant, deionized water, a water retaining agent and the like. By application of the compound microbial agent for low-temperature fermentation of straws, the straws can be decomposed and blackened about 12 days under the low-temperature condition of 4-15 DEG C, the straws are basically thoroughly decomposed at a high speed; due to addition of the auxiliary materials including the bacterium preserving agent, the carrier, the antioxidant, the water retaining agent and the like into the compound microbial agent for low-temperature fermentation of the straws, the compound microbial agent can be stored for a long time at the normal temperature.

A Pediococcus acidilactici strain that is viable at temperatures above 65° C., is able to grow in aerobic and anaerobic conditions, and in a pH range between 1 and 6.2 and a consumable food product containing same.

The invention discloses a novel strain ZPA017 of pediococcus acidilactici. The novel strain ZPA017 is assigned the accession number CGMCC No. 9040, and the novel strain ZPA017 has the advantages of being resistant to acid and bile salt, high in adhesion and good in antibacterial property. The invention further discloses a preparation containing the pediococcus acidilactici ZPA017. The preparation serves as a feed additive to feed weaned piglets and can inhibit reproduction of harmful bacteria and regulate intestinal microecology balance, and the preparation has effects of enhancing organism immunity and improving production performance.

The invention discloses a preparation method for a Szechuan pickled tuber vegetable fermentation composite microbial agent. The microbial agent comprises microbe powder 1, microbe powder 2 and microbe powder 3; the microbe powder 1 is prepared by inoculating 10L of Bacillus coagulans CICC 10144 strain culture solution in a logarithmic metaphase into a 1,000L airlift fermentor for high-density culture; the microbe powder 2 is prepared by inoculating 10L of Leuconostoc mesenteroides CGMCC 1.20 strain culture solution in a logarithmic metaphase into a 1,000L fermentor for culture; the microbe powder 3 is prepared by inoculating 5L of Lactobacillus plantarum CICC 6067 culture solution, 5L of Lactobacillus brevis CICC 6042 culture solution and 5L of Pediococcus acidilactici CGMCC 1.4 culture solution in a logarithmic metaphase into a 1,000L fermentor for co-culture; and the three kinds of microbe powder are mixed in a ratio of 2:5:5 and the mixture is subjected to vacuum packaging to form the Szechuan pickled tuber vegetable fermentation composite microbial agent. The problem that a microbial agent for producing Szechuan pickled vegetables at present is lacked is solved, and productioncontrollability and standard operation for industrially producing the Szechuan pickled vegetables are realized.

The invention relates to the field of fish foodstuff processing, in particular to instant cold sour fish and a preparation method thereof. The cold sour fish comprises, by weight, 95-105 parts of fillets, 1-2.5 parts of salt, 0.05-0.5 part of citrus cooking wine, 0.02-0.6 part of citrus seasoning vinegar, 0.01-0.4 part of citrus fish steaming extract, 0.5-5 parts of mixed sauce, 2-3 parts of staphylococcus xylosus liquid and 1-2 parts of mixed culture liquid of lactobacillus plantarum and pediococcus acidilactici, wherein the salt, the citrus cooking wine, the citrus seasoning vinegar and the citrus fish steaming extract serve as curing materials. The cold sour fish is manufactured through the steps of vacuum rolling, malaxating and curing, cold air drying, freezing, settling and slitting, curing and fermentation, vacuum package and cold sterilization. According to the instant cold sour fish and the preparation method thereof, the unique citrus fish extract, the citrus cooking wine and the citrus seasoning vinegar are adopted, the citrus added value is increased, technologies such as small-patch inoculation, segmented inoculated fermentation cultures, fermentation while ventilation and vacuum package are adopted, so that the producing time of the produced cold sour fish is shortened, flavor substances of the product are abundant, and the industrialized production of tradition foodstuff is achieved.

The invention discloses a preparation method of lactic acid, which takes lignocellulose as raw material, and is low in cost and high in efficiency, wherein the preparation method specifically comprises the following steps of: separating from lignocellulose to store pediococcus acidilactici, carrying out liquid culture on the pediococcus acidilactici to obtain cell bacteria taken as seeds, inoculating into a reactor with high lignocellulose content, adding cellulase, and synchronously carrying out saccharification and high-temperature fermentation to generate the lactic acid; or firstly saccharifying the cellulase of the lignocellulose to obtain sugar-containing hydrolysate, then carrying out high-temperature lactic acid fermentation by the hydrolysate to obtain the lactic acid; or uniformly blending the pediococcus acidilactici and the lignocellulose raw material, carrying out solid state cultivation, adding the pediococcus acidilactici-containing lignocellulose raw material into a fermentation tank, adding the cellulase, and synchronously carrying out saccharification and high-temperature fermentation to prepare the lactic acid. By adopting unique fermentation technology and microorganism, the preparation method is high in product concentration, simple in process, and low in cost; and particularly by taking the lignocellulose as raw material, the preparation method is wide in production space and development prospect.

The invention discloses a method for extracting proteins and chitin from shrimp heads and shrimp shells by pediococcus acidilactici, which comprises the following steps: (1) selecting fresh or frozen shrimp heads and shrimp shells, and unfreezing the frozen shrimp heads and the frozen shrimp shells; (2) adding carbon sources into the shrimp heads and the shrimp shells; (3) inoculating the pediococcus acidilactici; (4) carrying out heat-insulation fermentation; (5) separating fermentation liquid from residue after the fermentation is completed, and obtaining protein powder after the fermentation liquid is dried; and (6) carrying out diluted acid soaking and decolourization on fermentation residue to obtain chitin. Through the method of the invention, the proteins and the chitin in the shrimp heads and the shrimp shells can be simultaneously extracted out, and the obtained hydrolyzed proteins do not have stinking odor, and have rich calcium lactate and other mineral substances. The method of the invention has the advantages of simple operation method, production cost reduction and environmental pollution reduction, and rich nutritional ingredients contained in the shrimp heads and the shrimp shells can be sufficiently utilized.