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Composite microbial agent and preparation method and application thereof

A technology of compound microbial inoculants and pseudomonas preparations, applied in the field of environmental microorganisms, can solve problems such as secondary pollution

Inactive Publication Date: 2017-06-30
JIANGSU SUNTIME ENVIRONMENTAL REMEDIATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the chemical flocculation treatment technology, methods such as adding iron salts to promote phosphorus precipitation and adding lime for denitrification, although effective and high in efficiency, are likely to cause secondary pollution

Method used

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  • Composite microbial agent and preparation method and application thereof
  • Composite microbial agent and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: Screening and performance measurement, cultivation and preservation of Pseudomonas CGMCC No.13433

[0048] (1) Screening and performance determination of Pseudomonas CGMCC No.13433

[0049] The Pseudomonas CGMCC No.13433 is a highly efficient heterotrophic nitrification-aerobic denitrification bacterium isolated and selected from the activated sludge in the sewage treatment tank in Shanghai. Monascus, preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures. The specific screening process is as follows:

[0050] The activated sludge was taken to the sewage treatment tank in Shanghai sewage treatment tank, and 5 mL of mud-water mixture was taken from the activated sludge into 45 mL of sterilized heterotrophic nitrification medium. The formula of heterotrophic nitrification medium is: (NH 4 ) 2 SO 4 0.945g / L, sodium citrate 6.536g / L, MgSO 4 ·7H 2 O 1g / L, NaCl 0.12g / L, MnSO 4 ·H 2 O 0.01g / L, FeSO ...

Embodiment 2

[0059] Preparation of composite microbial agent for in-situ remediation of urban and rural river sewage

[0060] (1) Get Pseudomonas CGMCC No.13433 seeds, inoculate in solid medium I, through activation culture, activation condition is: 37 ℃, activation culture 48h, make activated Pseudomonas CGMCC No.13433; Then The activated Pseudomonas CGMCC No.13433 was inoculated into liquid medium I for expanded culture, and the expanded culture conditions were as follows: under the rotating speed of 180 rpm in a rotary shaker, cultivated at 30°C for 48 hours; 10% of the volume was inoculated in the fermentation medium I, and after cultivating at 32° C. for 96 h under a rotary shaker at 150 rpm, the Pseudomonas preparation was prepared, and the Pseudomonas thalline concentration was 4.0×10 9 cfu / mL.

[0061] Among them, solid medium I contains peptone 1g / 100mL, yeast powder 1.5g / 100mL, glucose 2g / 100mL, dipotassium hydrogen phosphate 0.1g / 100mL, agar 2g / 100mL, pH 6-8;

[0062] Liquid m...

Embodiment 3

[0098] Application of compound microbial agent in in-situ remediation of urban and rural river sewage

[0099] In a certain river in the suburbs of Shanghai, the river water is black and emits a strong odor. COD, ammonia nitrogen and total phosphorus exceed the standard. Take a 2L water sample from the river, add 2mL of liquid compound microbial agent, add the agent once every three days, and after three weeks of continuous feeding, the measurement results show that the COD of the water body has dropped from 265mg / L before the microorganisms were introduced to 15.3mg / L ;Ammonia nitrogen content dropped from 16.8mg / L before restoration to 0.69mg / L; total nitrogen dropped from 31.53mg / L to 0.97; total phosphorus dropped from 2.82mg / L to 0.23. Water environment quality standard V class water quality standard. Showed obvious water restoration effect (see Table 1)

[0100] Table 1 Remediation effect of compound microbial agents on river sewage

[0101] COD (mg / L) N...

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Abstract

The invention discloses a composite microbial agent and a preparation method and application thereof. The composite microbial agent includes a pseudomonas preparation, an acinetobacter preparation, a bacillus amyloliquefaciens preparation, a bacillus subtillis preparation, a bacillus licheniformis preparation, a pediococcus acidilactici preparation, a rhodobacter sphaeroides preparation, an alkali-lake dietzia preparation and a rhodopseudomonas palustris preparation. The preparation method includes the following steps that 1, composite microbial agent components are prepared; 2, the composite microbial agent components are mixed in proportion to obtain the composite microbial agent. The composite microbial agent can simultaneously decompose different organic matter, degrade amino nitrogen and nitro nitrogen in water, reduce phosphorus concentration in the water body and inhibit the growth of algae. The composite microbial agent is used for urban-rural sewage in-situ remediation, has the advantages of being high in efficiency, quick to take effect, free of no secondary pollution, simple in operation and the like, and large-scale application and popularization are facilitated.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and relates to a composite microbial bacterial agent for in-situ restoration of urban and rural river sewage, a preparation method and application thereof. Background technique [0002] Urban rivers and lakes are water environments closely connected with human production and life. As an important part of urban ecosystems, they have the functions of supplying water, improving the environment, providing green space, cultural education, entertainment and leisure, etc., and play an important role in urban ecological construction. significance. However, with the continuous acceleration of the urbanization process, the urban population gathers, the economic activities become more and more frequent, the discharge of industrial wastewater and domestic sewage increases, and the random discarding of garbage leads to the continuous increase of organic matter, ammonia nitrogen, total pho...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C12R1/07C12R1/125C12R1/10C12R1/01C02F103/00
CPCC02F3/341C02F2103/007C12N1/20
Inventor 唐晓声邹权
Owner JIANGSU SUNTIME ENVIRONMENTAL REMEDIATION
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