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Composite microbial flora and enzyme product thereof, and method of applying composite microbial flora and enzyme product thereof in soil restoration

A technology of compound microbial bacteria and microbial flora, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of soil quality decline, environmental pollution, etc., to promote crop growth, reduce application amount, improve The effect of soil fertility

Inactive Publication Date: 2015-12-23
TERRABIOTA BEIJING AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned defects and problems of soil quality decline and serious environmental pollution in the prior art, the purpose of the embodiments of the present invention is to provide a more reliable complex microbial flora and its enzyme products and its application method in soil restoration , repair polluted soil, improve soil, improve soil fertility and further regulate plant growth, increase plant yield, prevent and control plant diseases and insect pests, and improve plant disease resistance

Method used

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  • Composite microbial flora and enzyme product thereof, and method of applying composite microbial flora and enzyme product thereof in soil restoration
  • Composite microbial flora and enzyme product thereof, and method of applying composite microbial flora and enzyme product thereof in soil restoration
  • Composite microbial flora and enzyme product thereof, and method of applying composite microbial flora and enzyme product thereof in soil restoration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of powders of composite microbial flora and its enzyme products

[0046] 1. Activation and expansion of bacteria

[0047] Activation: Inoculate Bacillus subtilis on No. 1 medium and place it in a 28°C incubator for 5 days to obtain activated Bacillus subtilis; No. 1 medium is corn agar medium; for example, China Common Microbial Culture Collection Management Center (CGMCC for short) corn agar medium numbered 2006;

[0048] Inoculate Bacillus jelly on No. 2 medium and place it in an incubator at 28°C for 5 days to obtain activated Bacillus jelly; No. 2 medium is Ahri nitrogen-free medium, such as common Chinese microorganisms A nitrogen-free medium with the number 0073 of the culture preservation management center (CGMCC);

[0049] The powdered Pichia pastoris was inoculated on No. 3 medium and placed in an incubator at 28°C for 5 days to obtain activated powdered Pichia pastoris; No. 3 medium was LB medium, such as the preservation and management of commo...

Embodiment 2

[0056] Example 2 Application of composite microbial flora and its enzyme product powder to determine soil remediation effect

[0057] (1) Test medicine

[0058] According to the powder of the composite microbial flora and its enzyme products obtained in Example 1 of the present invention, it is dissolved in warm water to activate the bacterial species before use to obtain a biological bacterial fertilizer made of the composite microbial flora and its enzyme products.

[0059] (2) Test background

[0060] The current soil pollution problem is getting more and more serious, and the current soil remediation program is either expensive, or it can reach the maximum acceptable limit (50 mg / kg) over a long period of time by regularly turning over 9 to 12 months. In the face of the current problems, in order to determine the remediation effect of the biological bacterial fertilizer preparation made of the composite microbial flora and its enzyme products of the present invention on contaminat...

Embodiment 3

[0073] Example 3 Fertilizer effect test of composite microbial flora and its enzyme products on greenhouse tomatoes

[0074] (1) Test medicine

[0075] Powders and waters prepared according to the composite microorganism enzyme system of the present invention.

[0076] (2) Experimental design

[0077] In a greenhouse, tomato plants with a quantitative number of seats were randomly selected as the experimental group and the control group. The experimental group was divided into two groups and managed with the same field management measures. The experimental group adopted two application schemes. Application scheme 1 was to add 50g of nitrogen fertilizer each time the bacterial fertilizer was applied; application scheme 2 was to apply the normal bacterial fertilizer and apply the same amount of water to the control group. The records of bacterial fertilizer application are as follows:

[0078] 4 days before planting, the experimental group and the control group were treated with organic...

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Abstract

The invention provides a composite microbial flora and an enzyme product thereof, and a method of applying the composite microbial flora and the enzyme product thereof in soil restoration. The composite microbial flora is composed of a microbial flora and a bioactive enzyme, wherein the microbial flora is composed of bacillus subtilis, bacillus mucilaginosus, bacillus amyloliquefaciens, pediococcus pentosaceus, pichia farinose and pediococcus acidilactici, and the bioactive enzyme is composed of phytase, protein hydrolase, cellulose hydrolase and urease. Compared with the prior art, an effective microbial community and a microecological environment are constructed to realize biological catalysis and improve the content of organic matters and beneficial microbial flora in soil, and thus, the fertility of the soil is improved; biochemical degradation rate of soil pollutant can be substantially accelerated, digestion and biodegradation of the pollutant can be accelerated, and final inertial products like carbon dioxide, water and nitrogen are obtained; and toxic intermediate produced in the process of biochemical degradation can be rapidly removed, odor is eliminated, and emission of organic volatile matters (VOC) is reduced.

Description

Technical field [0001] The invention relates to the technical field of microbial soil conditioners, in particular to a composite microbial flora and its enzyme products and an application method in soil restoration. Background technique [0002] Modern agricultural production requires the sustainable development of agriculture. The sustainable development of agriculture requires the continuous improvement of soil fertility and the increase of soil organic matter content. The sustainability of agriculture can also be said to be the sustainability of soil. Soil sustainability indicators include soil nutrient elements, soil microbial diversity, biomass, biological adaptability, biological stability, enzyme diversity, etc. [0003] Soil is composed of minerals, organic matter and microorganisms, which are the basis for the growth and development of crops. Beneficial microorganisms in the soil directly participate in a series of soil fertility formation and development processes such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N9/80C12N9/50C12N9/42C12N9/16B09C1/10C09K17/00C05F11/08A01C21/00C12R1/125C12R1/84C12R1/01C09K101/00
Inventor 王闽龙潘灿平李子昂连文超
Owner TERRABIOTA BEIJING AGRI SCI & TECH
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