31 results about "Pichia farinosa" patented technology

The invention relates to a compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is prepared by mixing the following raw materials: yeast fermentation powder residues, an organic fertilizer, urea, K2SO4, NH4H2PO4, clay and a compound microbial agent; and the compound microbial agent is formed by fermentation of bacillus mucilaginosus, bacillus megaterium, bacillus cereus, bacillus thuringiensis, azotobacter chroococcum, pichia farinose and streptomyces microflavus. The compound microbial fertilizer disclosed by the invention has a variety of microbes with the effects of nitrogen fixation, phosphorus dissolution and potassium dissolution, can increase the nitrogen content in soil, improves the utilization rate of the chemical fertilizer, and is favorable for improving the structure of the soil and improving the yield and quality of crops; and simultaneously, the microbes in the fertilizer can produce a variety of metabolites, and the metabolites can effectively resist diseases and insect pests.

The invention provides a composite microbial flora and an enzyme product thereof, and a method of applying the composite microbial flora and the enzyme product thereof in soil restoration. The composite microbial flora is composed of a microbial flora and a bioactive enzyme, wherein the microbial flora is composed of bacillus subtilis, bacillus mucilaginosus, bacillus amyloliquefaciens, pediococcus pentosaceus, pichia farinose and pediococcus acidilactici, and the bioactive enzyme is composed of phytase, protein hydrolase, cellulose hydrolase and urease. Compared with the prior art, an effective microbial community and a microecological environment are constructed to realize biological catalysis and improve the content of organic matters and beneficial microbial flora in soil, and thus, the fertility of the soil is improved; biochemical degradation rate of soil pollutant can be substantially accelerated, digestion and biodegradation of the pollutant can be accelerated, and final inertial products like carbon dioxide, water and nitrogen are obtained; and toxic intermediate produced in the process of biochemical degradation can be rapidly removed, odor is eliminated, and emission of organic volatile matters (VOC) is reduced.

The invention relates to a complex microorganism fungicide for treating animal manure and a preparation method and an application thereof. The adopted technical scheme is that the complex microorganism fungicide consists of saccharomyces cerevisiae, powdery pichia, bacillus subtilis, bacillus cereus, bacillus megaterium and lactobacillus plantarum. Microbial organic fertilizer can be prepared by treating the animal manure with the complex microorganism fungicide. The complex microorganism fungicide for treating the animal manure prepared by the invention has the advantages of easy obtainment of raw materials and simple manufacturing method. Preparing the microbial organic fertilizer by mixing the complex microorganism fungicide for treating the animal manure with the animal manure, organic materials and cornmeal not only can treat the animal manure, but also effectively utilizes rural wastes; and the prepared microbial organic fertilizer can not pollute crops and cultivated land because no chemical substance exists in the prepared microbial organic fertilizer.

The invention discloses a strain produced by screening beta-damascenone in Chinese liquor brewing by utilizing flavor directional technology and application thereof, belonging to the fields of biology engineering technology and brewing biotechnology. The beta-damascenone is an important trace characteristic flavor substance in Chinese Fen-flavor liquor. The strain is any one of Pichia anomala CGMCC4740, Issatchenkia orientalis CGMCC4741, Saccharomycopsis fibuligera CGMCC4742, Pichia farinosa CGMCC4743, Schizosaccharomyces pombe CGMCC4744, Trichosporon asahii CGMCC4746, Saccharomyces cerevisiae CGMCC4747, Kazachstania exigua CGMCC4748, Hanseniaspora osmophila CGMCC4749, Pichia fermentans CGMCC4750, Pichia membranifaciens CGMCC4751 and Clavispora lusitaniae CGMCC4752. The strain is obtainedby separating Daqu, Xiaoqu, fermented grains and brewing materials in the brewing environment of many famous wineries in China. The strain can be applied to the field of traditional food brewing and preparation of beta-damascenone biological spices.

The invention provides a microbial compound agent for preparing bio-organic fertilizers with excrement. The microbial compound agent comprises the following components by weight percent: 40-50% of bacillus subtilis containing carrier, 5-10% of lactobacillus plantarum containing carrier, 8-15% of pichia farinose containing carrier, 15-25% of bacillus licheniformis containing carrier and 5-10% of nocardioides albus containing carrier. The microbial compound agent has the beneficial effects that the macromolecular matters of the excrement are decomposed into micromolecular matters to produce the bio-organic fertilizers through deodorization, thorough decomposition, dehydration and drying; the microbial compound agent is low in raw material costs and simple in production method.

The invention relates to a multidimensional complex enzyme inoculant as well as a preparation method and applications of the multidimensional complex enzyme inoculant. The preparation method comprisesthe following steps: mixing bacterial mixed fermentation liquor, a mould mixture and a saccharomycete mixture, thus obtaining a strain mixture; inoculating the strain mixture into a solid fermentation culture medium, thus obtaining a fermentation mixture; adjusting the moisture of the fermentation mixture, carrying out stacking to form a conical pile, and carrying out fermentation, thus obtaininga primary fermentation product; carrying out anaerobic fermentation on the primary fermentation product, thus obtaining a secondary fermentation product; and drying the secondary fermentation product, and carrying out grinding, thus obtaining the multidimensional complex enzyme inoculant. According to the technical scheme, various strains including bacillus subtilis, bacillus amyloliquefaciens,bacillus mucilaginosus, bacillus licheniformis, trichoderma harzianum, aspergillus oryzae, pichia farinose and an aroma-producing yeast are adopted as the fermentation strains, then the multidimensional complex enzyme inoculant containing various efficient enzymes, beneficial substances and beneficial microbial populations and having the high-activity fermentation function is prepared, and the multidimensional complex enzyme inoculant can be used for preparing organic fertilizers, feeds and feed additives.

The invention relates to a special microorganism strain for processing steel slag, vinegar residues and weathered coal and a preparation method thereof. The microorganism strain comprises hansenula anomala, film press hansenula, pichia farinose, saccharomyces cerevisiae, geotrichum candidum, bacillus subtilis withered grass subspecies, bacillus licheniformis, bacillus megaterium, lactobacillus acidophilusis and streptomyces microflavus in the mass ratio of 2:2:2:2:1:5:3:2:1:4. The preparation method comprises the steps of microorganism selection, activation, one-class expansion, second-class swinging bed amplification, carrier adsorption, preparation, low-temperature drying, detection, metering and packaging. The invention can harmlessly process the steel slag, vinegar residues and weathered coal, neutralizes the strong alkalinity of the steel slag by utilizing the organic acid generated in the process of fermentation, forms an organic acid metal complex, reduces the fixation of nutrients, improves the effective utilization ratio of the nutrients, efficiently utilizes organic sources, such as steel slag, vinegar residues, weathered coal, and the like, and reduces environmental pollution.

The present invention particularly discovered strains that are capable of producing a long-chain dicarboxylic acid by culturing microorganisms belonging to Candida vini Candida entamophila, Candida blankii and Pichia farinosa which has the ability to produce a long-chain dicarboxylic acid in a liquid medium containing a straight-chain saturated hydrocarbon (tridecane) as substrate.

The invention relates to the field of microorganisms, particularly an active microorganism combination biological agent and a preparation method thereof. The active microorganism combination biological agent is prepared by mixing Pediococcus acidilactici, Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactobacillus plantarum, Bacillus amyloliquefaciens, Bacillus subtilis, Lactobacillus acidophilus, Pichia farinosa, Pichia membranifaciens, Dekkera anomala and Dekkera bruxellensis. The biological agent has the advantages of high treatment efficiency for pollutants, favorable treatment effect, low treatment cost and no secondary pollution.

The invention discloses water-soluble biological organic fertilizer. The water-soluble biological organic fertilizer is prepared from the following components: chicken manure, potassium fulvate, amino acid, an organic material and a compound flora, wherein the organic material comprises one of starch or brown sugar or combination thereof, and rice bran; the compound flora is prepared from the following components: bacillus subtilis, pediococcus pentosaceus, aspergillus niger, bacillus mucilaginosus, rhizopus oryzae, pichia farinosa, sphingobacterium multivorum and ferment bacteria. By combining ferment bacterium strains and fermenting the organic material and the chicken manure, the water-soluble biological organic fertilizer has abundant useful microbiological florae; the solubility is improved after sufficient fermentation; the potassium fulvate and the amino acid are added and are mixed and stirred after a fermented product is dried; then a mixture is crushed and is ground and sieved, so that the water solubility is guaranteed. After the water-soluble biological organic fertilizer is applied, nutrients can be supplemented in time and long fertilizer efficiency time is guaranteed; the water-soluble biological organic fertilizer is convenient to use and manpower and material resources are effectively saved.

The invention discloses a river course water body and sediment in-situ remediation process using an enzyme-bacterial microbial agent, wherein the in-situ remediation process comprises: 1, raw materialselection; 2, bacterial activation; 3, bacterial fermentation; 4, enzyme and bacterial compounding; 5, sediment tedding; 6, sediment backfilling; and 7, microbial agent activation. According to the present invention, the remediation agent is formed by compounding Pediococcus acidi lactici, Enterococcus faecalis, Bacillus amyloliquefaciens, Pichia farinosa and Dekkera anomala based on five beneficial biomass such as hemicellulase, xylanase, amylase, pectinase and Arabic gum enzyme, is a strain having good adaptability to river course water body and sediment, has good organic matter degradationeffect, and can effectively remove COD, ammonia nitrogen, total phosphorus, total nitrogen and various organic pollutants from river course water bodies so as to improve the water quality and the substrate environment of the water body, easily achieve the natural construction of the aquatic ecosystem, and achieve the in situ restoration of water bodies and sediments.

The invention relates to a pesticide-fertilizer including: rice vinegar, brown sugar, monopotassium phosphate, calcium superphosphate, urea, magnesium sulfate, manganese sulfate, a boron fertilizer, zinc sulfate, soya beans, multiple microorganisms and water. The multiple microorganisms include: bacillus subtilis, pediococcus pentosaceus, aspergillus niger, bacillus mucilaginosus, rhizopus oryzae, pichia farinosa, sphingobacterium multivorum and enzyme microorganism. In the pesticide-fertilizer, the effective functional multiple microorganisms can inhibit living space of pathogens and secrete various substances that can inhibit the pathogens, so that the pesticide-fertilizer has the effect of prevention and treatment of diseases; the multiple microorganisms have excellent compatibility with the rice vinegar and other components, and quality of the pesticide-fertilizer can be improved further by means of a spray solution containing a multiple microorganism liquid and the rice vinegar. The pesticide-fertilizer has more excellent effect on prevention and treatment of crop diseases and sterilization. Two field operations, crop protection and nutrient supplement by fertilizers, are integrated, so that labor intensity and production cost are reduced.

The invention relates to a highly-active Pichia pastoris powder and a preparation method thereof. The preparation method comprises the steps of activation culture, primary seed culture, preparation of a high-density liquid fermentation medium, fermentation culture, material supplementing and auxiliary material addition. The preparation method can substantially the output per unit by about 30%, reduces the cost per unit by about 20%, and greatly improves the device utilization rate; a composite protection agent and a carrier maximally protect Pichia pastoris in order to obtain the highly-active Pichia pastoris powder; and compared with traditional centrifuge and spray drying production technologies, the preparation method disclosed in the invention, adopting a cold air drying technology, has the advantages of low drying temperature, satisfactory reservation of the bioactivity, overcoming of the disadvantages of low living bacteria and high investment, and wide market prospect.

The invention relates to the field of organic fertilizer fermentation, and especially relates to an organic fertilizer complex bacteria leaven, a special device thereof, and an application of the organic fertilizer complex bacteria leaven in the quick-ripening pre-fermentation of an organic fertilizer. The complex bacteria leaven comprises mixed bacteria A and mixed bacteria B, the mixed bacteriaA comprise Pediococcus pentosaceus, Pichia farinosa and high temperature yeast, and the mixed bacteria B comprise Bacillus licheniformis, Bacillus pallidus and Bacillus laterosporus. The quick-ripening pre-fermentation device comprises a fermentation tank, a heat source comprehensive utilization unit, a deodorizing, cooling and purifying unit, a wastewater comprehensive utilization unit, a coolingsystem and a temperature control system. The humidity, temperature, dissolved oxygen and the like in the fermentation process are effectively regulated, and efficient decomposing microorganisms are combined, so the growth and the metabolism of organic fertilizer decomposing microorganisms are greatly improved, thereby physical crushing and biochemical degradation of organic materials are accelerated, and the harmless treatment process of the materials and the decomposing and mineralizing process of the organic materials are greatly shortened.

The invention relates to the field of breeding, and particularly relates to an animal feed additive as well as a preparation method and application thereof. The animal feed additive is prepared by mixing the following components: Pediococcus acidilactici, Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactobacillus plantarum, Bacillus amyloliquefaciens, Bacillus subtilis, Lactobacillus acidophilus, Pichia pastoris, Pichia membranaceus, Dekkera, and Dekkera bruxellensis. The preparation method provided by the invention can improve a survival rate of animals, accelerate growth of the animals, and improve quality of meat without affecting human health.

The invention discloses Pichia farinosa NHG and application of the Pichia farinosa to in the degradation of ammonia nitrogen. The preservation registration number of the Pichia farinosa NHG provided by the invention is CGMCC (China General Microbiological Culture Collection Center) NO.10561. The invention also protects a culture and a bacterial suspension of the Pichia farinosa NHG. The invention further protects a product; active ingredients of the product are the Pichia farinosa NHG, the culture or the bacterial suspension; the functions of the product are described as (a) or, (b) or, (c) or (d): (a) degrading the ammonia nitrogen; (b) degrading ammonia nitrogen in water; (c) degrading ammonia nitrogen in excrement; (d) reducing the emission of ammonia gas in the excrement, wherein the excrement can be livestock excrement, such as chicken manure. The Pichia farinosa provided by the invention can be applied to the following aspects of (1) improving the water quality problem of water polluted by the ammonia nitrogen, (2) improving the environmental sanitation in livestock and poultry houses, and (3) being used as a feed additive of chicken.

The invention discloses a method for treating biomass pyrolysis effluent by utilizing microorganism bacterial strains. The method comprises the following steps: 1) preparing a culture medium; 2) preparing bacterial strains; 3) pretreating the biomass pyrolysis effluent; 4) fermenting the biomass pyrolysis effluent. According to the invention, the microorganism bacterial strains are utilized to treat the biomass pyrolysis effluent; cost is low; the adopted microorganism bacterial strains include bacillus subtilis, clostridium acetobutylicum, streptosporangium, white rot fungi, aspergillus nigerand powdered pichia pastoris; through reasonable blending of the microorganism bacterial strains, the biomass pyrolysis effluent is comprehensively fermented; the fermented products only include carbon dioxide and water and are environment-friendly and pollution-free; the method for treating biomass pyrolysis effluent provided by the invention is simple and convenient in operation, is capable ofthoroughly treating effluent and is capable of solving the problem of environmental pollution caused by the biomass pyrolysis effluent.

The invention discloses a bio-organic fertilizer special for tea. The bio-organic fertilizer is prepared from organic materials containing lignin, superphosphate, rice bran, multiple microorganisms and brown sugar, wherein the multiple microorganisms comprises the components of bacillus subtilis, pediococcus pentosaceus, aspergillus niger, bacillus mucilaginosus, rhizopus oryzae, powdery pichia pastoris, sphingobacterium multivorum and enzyme microorganism. According to the bio-organic fertilizer, a microorganism technology is applied to cultivation of tea trees, good organic matter materials are applied after being fermented under the actions of effective microorganisms and enzymes thereof, so that nutrients are supplemented, soil is improved effectively at the same time, growth of harmful microorganisms is restrained through large amount breeding of organic microorganisms in soil, the stress resistance of the tea trees is improved, and the occurrence of plant diseases and insect pests is reduced.

The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a high-density fermentation process of powdery Pichia pastoris, which includes seed preparation and fermentation tank cultivation. Fermentation tank culture conditions: liquid volume 40%-60% (V / V), inoculum volume 1%-5% (V / V), control temperature 28-40°C, ventilation volume 1-3.5VVM, initial speed 300r / min, increase the speed by 100r / min every 4h, and keep it unchanged when it is increased to 700r / min, and the fermentation cycle is 20-30h. During the fermentation process, the pH of the fermentation broth was adjusted to about 6.0 by adding NaOH solution dropwise. After 13 hours of fermentation, a certain amount of glucose was added intermittently, and the fermentation ended after 20-30 hours, and the effective number of viable bacteria was 3.00-3.50 billion / mL. By adopting the fermentation process method of the present invention, not only the amount of bacteria is significantly and greatly increased, but also the process is simple, the efficiency is better than that of the prior art, and the production cost is lower.

The invention provides microorganism fertilizer capable of preventing and treating insect pests of crops. The following components are added into each 100kg of water in parts by weight: 0.5kg to 0.8kg of complex bacteria flora, 0.6kg to 1.1kg of rice vinegar, 0 to 0.12kg of calcium superphosphate, 0 to 0.1kg of potassium dihydrogen phosphate, 0 to 0.07kg of ferric sulfate, 0 to 0.08kg of pure boron, 0 to 0.3kg of garlic and 0 to 1kg of chilies, wherein the complex bacteria contain the following components: bacillus subtilis, pediococcus pentosaceus, aspergillus niger, bacillus mucilaginosus, rhizopus oryzae, pichia farinose, sphingobacterium multivorum and ferment bacteria. The microorganism fertilizer capable of preventing and treating the insect pests of the crops is used for carrying out foliar spraying on the crops through adding the rice vinegar, the calcium superphosphate, the ferric sulfate, the potassium dihydrogen phosphate, the garlic, the chilies and the like into the complex bacteria flora, so that the thickness of a wax layer in plants is increased, the stress resistance of the crops is enhanced, an insecticidal spectrum is wide and the insecticidal rate is high; the raw materials are cheap and easy to obtain; a production method is simple and easy to grasp, is suitable for industrial production and can also be applicable to domestic production of biological organic fertilizer of farmers.

The invention relates to a method for producing a safe high-biomass lactobacillus product by plant-derived multi-microbe mixed fermentation, belonging to the field of bioengineering. The fermentation strain in the fermentation production method of the lactobacillus product is an associate strain composed of Lactobacillus casei, Lactobacillus plantarum and powdery Pichia pastoris. The associate strain is sequentially subjected to primary amplification culture and secondary amplification culture, and used in large-scale production of the lactobacillus product at the inoculum size of 10-15%. The method reasonably utilizes the synbiosis among the microbes, the fermentation strain used in fermentation has higher controllability, and the end product has higher biomass; and therefore, the obtained lactobacillus product is safer and more efficient when being used in animal culture, and thus, is suitable for wide application in agricultural production.

The invention discloses a pichia farinose strain FL7CGMCC N0.4008 which can dissolve inorganic phosphorus compounds and application thereof. The experimental results show that the pichia farinose strain FL7CGMCC N0.4008 has the functions of dissolving inorganic phosphorus compounds and using celluloses, and has good tolerance to high temperature, high NaCL concentration, high ammonium ion concentration and alkaline environment.

The invention discloses Pichia farinosa NHG and application of the Pichia farinosa to in the degradation of ammonia nitrogen. The preservation registration number of the Pichia farinosa NHG provided by the invention is CGMCC (China General Microbiological Culture Collection Center) NO.10561. The invention also protects a culture and a bacterial suspension of the Pichia farinosa NHG. The invention further protects a product; active ingredients of the product are the Pichia farinosa NHG, the culture or the bacterial suspension; the functions of the product are described as (a) or, (b) or, (c) or (d): (a) degrading the ammonia nitrogen; (b) degrading ammonia nitrogen in water; (c) degrading ammonia nitrogen in excrement; (d) reducing the emission of ammonia gas in the excrement, wherein the excrement can be livestock excrement, such as chicken manure. The Pichia farinosa provided by the invention can be applied to the following aspects of (1) improving the water quality problem of water polluted by the ammonia nitrogen, (2) improving the environmental sanitation in livestock and poultry houses, and (3) being used as a feed additive of chicken.

The invention discloses a pichia farinose strain FL7CGMCC N0.4008 which can dissolve inorganic phosphorus compounds and application thereof. The experimental results show that the pichia farinose strain FL7CGMCC N0.4008 has the functions of dissolving inorganic phosphorus compounds and using celluloses, and has good tolerance to high temperature, high NaCL concentration, high ammonium ion concentration and alkaline environment.

The invention relates to a complex microorganism fungicide for treating animal manure and a preparation method and an application thereof. The adopted technical scheme is that the complex microorganism fungicide consists of saccharomyces cerevisiae, powdery pichia, bacillus subtilis, bacillus cereus, bacillus megaterium and lactobacillus plantarum. Microbial organic fertilizer can be prepared by treating the animal manure with the complex microorganism fungicide. The complex microorganism fungicide for treating the animal manure prepared by the invention has the advantages of easy obtainment of raw materials and simple manufacturing method. Preparing the microbial organic fertilizer by mixing the complex microorganism fungicide for treating the animal manure with the animal manure, organic materials and cornmeal not only can treat the animal manure, but also effectively utilizes rural wastes; and the prepared microbial organic fertilizer can not pollute crops and cultivated land because no chemical substance exists in the prepared microbial organic fertilizer.

Provided is an animal feed for preventing and treating infectious diseases, which has a prophylactic / therapeutic effect on infectious diseases caused by viruses, and is inexpensive and free from side effects. The animal feed comprises at least three of the following microorganisms, preferably including all of the following microorganisms: a) Pediococcus pentosaceus, b) Pediococcus acidilactici, c) Pichia farinosa, d) Dekra bruxellensis, e) Bacillus (Bacillus) (Bacillus), f) Streptococcus (Streptococcus), and g) Staphylococcus (Staphylococcus). The animal feed comprises at least three of the following microorganisms, preferably including all of the following microorganisms: a) Pediococcus pentosaceus, b) Pediococcus acidilactici, c) Pichia farinosa, d) Streptococcus (Streptococcus).

The invention relates to an agricultural microbial agent containing rhodobacter azotoformans, lactobacillus plantarum, pichia farinosa, amino acid and maltitol as well as a preparation method and application of the agricultural microbial agent. According to the invention, marine trash fish and soybean meal which are rich in protein are used as main raw materials, maltitol and molasses powder are used as auxiliary materials, and after reasonable compounding, different proteins in the raw materials are decomposed under specific fermentation conditions, so the agricultural microbial agent which takes various thalli, amino acid, polysaccharides and the like as efficient components is prepared. The agricultural microbial agent can effectively improve the micro-ecological environment of soil and promote the conversion of organic matters in the soil, and various bacteriostatic active substances generated by beneficial microorganisms can enhance the ability of crops to resist various diseases, reduce the occurrence of soil-borne diseases and promote the robust growth of plants. The agricultural microbial agent can effectively promote the formation of a soil aggregate structure, enables the soil to become loose and fertile, and is beneficial to cultivation and growth and development of crop roots. The agricultural microbial agent contains rich organic matters, and can activate plough layer soil, improve the water retention capacity of the soil, enhance the adsorption effect of the organic matters, absorb and accommodate various nutrient substances, effectively avoid nutrient loss, improve the fertilizer utilization rate and enhance the fertilizer retention and supply capacity and buffer capacity of the soil.