Agricultural microbial agent and preparation method thereof
A technology of microbial inoculum and Lactobacillus plantarum, applied in the field of agricultural microbial inoculum and preparation, can solve problems such as unsatisfactory effect, ineffectiveness, poor synergy ability, etc., and achieves improved crop yield and quality, short production cycle, The effect of promoting synthesis
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Embodiment 1
[0031] Embodiment 1: a kind of preparation method of polypeptide agricultural microbial inoculum, the steps are as follows:
[0032] (1) Preparation of seed liquid: Inoculate the nitrogen-fixing rhodobacter bacteria cultured on RCVBN slant medium, Lactobacillus plantarum cultured on MRS slant medium, and powdery Pichia pastoris cultured on YED slant medium into the seed medium respectively. Cultivate at 28°C for 96 hours to obtain seed liquids of Rhodobacter nitrogen-fixing bacteria, Lactobacillus plantarum, and Pichia powderylum respectively. 3×10 10 CFU / mL, 2×10 10 CFU / mL, 1×10 10 CFU / mL;
[0033] (2) Preparation of pretreatment mixture: in parts by weight, add 40 parts of soybean meal powder and 20 parts of sea fish to adjust the moisture content to 70wt%, let stand at 28°C for 12 hours, then place at 115°C Cook for 20 minutes, after cooling, add 20 parts of molasses powder and 15 parts of maltitol and mix evenly to obtain a pretreatment mixture, which is set aside;
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Embodiment 2
[0036] Embodiment 2: a kind of preparation method of polypeptide agricultural microbial inoculum, the steps are as follows:
[0037] (1) Preparation of seed liquid: Inoculate the nitrogen-fixing rhodobacter bacteria cultured on RCVBN slant medium, Lactobacillus plantarum cultured on MRS slant medium, and powdery Pichia pastoris cultured on YED slant medium into the seed medium respectively. Cultivate at 30°C for 72 hours to obtain the seed liquids of Rhodobacter nitrogen-fixing bacteria, Lactobacillus plantarum and Pichia powderylum respectively. 2×10 10 CFU / mL, 1×10 10 CFU / mL, 1×10 10 CFU / mL.
[0038] (2) Preparation of pretreatment mixture: in parts by weight, add 50 parts of soybean meal powder and 15 parts of sea fish to adjust the water content to 80wt%, let stand at 25°C for 24 hours, then place at 121°C Cook for 20 minutes, after cooling, add 10 parts of molasses powder and 30 parts of maltitol and mix evenly to obtain a pretreatment mixture, which is set aside;
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