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High-density cultivation method for lactobacillus

A technology for high-density cultivation of lactic acid bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as high production costs and unguaranteed strain quality

Inactive Publication Date: 2008-08-20
丁之铨 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high nutritional requirements for the growth of lactic acid bacteria, some special nutritional components are needed; at the same time, lactic acid bacteria secrete organic acid metabolites during the growth process, which makes the pH value in the culture solution continuously decrease. When the pH value drops below 4.10, The growth and reproduction of bacteria will be inhibited, so the traditional production process of bacteria can not guarantee the quality of bacteria, and the production cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Pick the activated Lactobacillus plantarum in the MRS liquid medium, cultivate it at 37°C for 12 hours, and inoculate it into a fermentation tank containing lactic acid bacteria medium at an inoculum of 6%. The initial pH of the medium is 7.0. When the pH is lower than 6.6 hour flow plus 30% Na 2 CO 3 Solution, keep the pH value of the culture medium stable at 6.7±0.1, and incubate at 37°C for 30h.

Embodiment 2

[0009] Pick the activated Lactobacillus plantarum in MRS liquid medium, cultivate it at 37°C for 12h, and inoculate it into a fermenter containing lactic acid bacteria medium at an inoculum of 6%. The initial pH of the culture solution is 7.0. When the pH is lower than At 6.6, add 20% NaOH solution to keep the pH of the culture medium at 6.7±0.1, and incubate at 37°C for 30 hours.

Embodiment 3

[0011] Pick the activated Lactobacillus bulgaricus in the MRS liquid medium, cultivate it at 37°C for 12 hours, and inoculate it into the fermenter containing the lactic acid bacteria medium at an inoculum of 10%. The initial pH of the culture solution is 7.2. When the pH is lower than At 6.0, add 20% KOH solution to keep the pH of the culture medium at 6.2±0.2. Cultivate at 42°C for 24h.

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Abstract

The invention relates to a high-density culture method of lactic acid bacteria, which takes Lactobacillus plantarum, Lactobacillus bulgaricus and Pediococcus acidilactici as bacterial strains, a special lactic acid bacteria culture medium is used, the basic formula includes: 3 percent soybean peptone, 2 percent yeast extract, 0.5 percent sodium acetate, composite trace element liquid and composite vitamin solution, etc., the initial pH value is regulated to 7.0 to 7.2; Na2CO3, KOH, NaOH or ammonia is fed during the culture process to allow the pH value of a culture liquid to be maintained at 6.0 to 6.8. The inhibition of the growth of the self-metabolic product is eliminated, the high-activity and high-concentration lactic acid bacteria culture medium is obtained, and the living bacterial number of the lactic acid bacteria in the culture liquid can achieve 9.0 multiplied by 10<9> to 1.0 multiplied by 10 <10> cfu / ml.

Description

Technical field [0001] The invention relates to a high-density culture method of lactic acid bacteria, and belongs to the field of microbial fermentation engineering. Background technique [0002] Lactic acid bacteria are part of the normal microflora or flora of humans and animals. Because of its special physiological functions, it is widely used in many industries such as food, medicine and feed. Lactic acid bacteria is an important microbial flora that produces fermented dairy products, kimchi, cheese, fermented sausages and other traditional fermented foods, giving them special texture, flavor and taste; lactic acid bacteria play an important role in livestock breeding and feed, especially in silage It plays a leading role in the fermentation process, which can effectively improve the fermentation quality of silage and improve the production performance of animals. [0003] The activity of lactic acid bacteria inoculum is closely related to its quantity. Therefore, in order t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12R1/225C12R1/25
Inventor 丁之铨林爱玉
Owner 丁之铨
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