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Molecule making method for gene locus for preventing mycosphaerella melonis of muskmelon

A technology of molecular markers and blight, which is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms, can solve the problems of few researches on the utilization of antibiotics, and achieve the effects of convenient identification, clear selection targets, and high cost

Inactive Publication Date: 2008-05-07
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] At present, the research on the genetic basis of resistance to scabies and the utilization of disease-resistant breeding are mostly concentrated on the earliest discovered resistance resource PI 140471, while there are few studies on the utilization of other resistance sources.

Method used

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  • Molecule making method for gene locus for preventing mycosphaerella melonis of muskmelon

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Embodiment Construction

[0033] The implementation procedure of screening muskmelon blight resistance gene molecular marker of the present invention comprises:

[0034] (1) F 1 (See references for PI 420145 and PI 136170: Wolukau et al., 2007, Resistance to Gummy Stem Blight in Melon (Cucumis melo L.) Germplasm and Inheritance of Resistance from Plant Introductions 157076, 420145, and 323498. HortScience 42(2): 215-221.), F 1 F 2 , while backcrossing produces BC 1 generation. F grown in the experimental farm of Nanjing Agricultural University in the spring of 2006 2 Generations and BC 1 generational segregation groups.

[0035] It was artificially inoculated and the incidence of each individual plant was counted. The chi-square test was used to analyze the genetic law of the resistance to the blight. Analysis found that 63 strains of BC 1 In the backcross population, 29 strains were disease-resistant and 37 strains were susceptible, and the segregation ratio was completely consistent with the ...

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Abstract

The invention relates to a molecular marker method for a melon blight resistance gene, belonging to the field of biotechnology. There are four AFLP molecular markers that are linked to the gene for resistance to blight blight, the marker EcoRI-TG / MseI-CTC200 is a 200bp band; the marker EcoRI-AT / MseI-CTG90 is a 90bp band; the EcoRI-TC / MseI-CAG60 is a 60bp band band; the marker EcoRI-TG / MseI-CTA70 is a 70bp band; the linkage distances are 2.0, 6.0, 5.4 and 6.0cM, respectively. Detect whether PI 420145 and its derivative varieties (lines) contain the gene through the molecular marker method provided by the present invention, predict its resistance level to vine blight, improve the selection efficiency, and help to accelerate the selection of excellent vine blight-resistant melon varieties in my country It is used in the production process to identify the purity of muskmelon varieties resistant to vine blight.

Description

1. Technical field [0001] The invention discloses a molecular marker method for a melon blight resistance gene locus, and belongs to the field of biotechnology. Dedicated to the molecular marker-assisted selection of muskmelon blight resistance, the breeding of muskmelon blight-resistant varieties and the utilization of germplasm resources. 2. Technical background [0002] Melon is one of the top ten fruits in the world. Melon blight is one of the most serious global fungal diseases. Its occurrence often leads to devastating consequences. In addition to harming melons, the fungus also infects other cucurbit crops such as cucumbers, watermelons, pumpkins and zucchini, resulting in different degrees of production reduction. Therefore, it is an urgent task in the current muskmelon breeding to study and prevent muskmelon vine blight. Breeding disease-resistant varieties is one of the safest, most effective and cost-effective measures to control the damage of muskmelon blight...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈劲枫鸠瑟夫·瓦鲁考周晓慧李英张永兵娄群峰钱春桃
Owner NANJING AGRICULTURAL UNIVERSITY
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