Molecular marker method for rice anti-rice stripe major gene loci qSTV11

Abstract

The invention relates to a molecular marking method of a major gene locus qSTV11 with rice stripe and leaf blight resistance and belongs to the field of molecular genetics. Hybridization with female plant of rice Nipponbare and male plant of Kasalath, backcross with Nipponbare and derivation are orderly realized, thus obtaining a backcross recombinant inbred system, genetic linkage analysis between genotypes and corresponding disease indexes and ratios in each family is realized, the resistant effects of the major gene locus qSTV11 with rice stripe and leaf blight resistance and allelic genes from Kasalath are detected, and molecular markers R21, R22, R13 and R48 that can be utilized in breeding are obtained through filtering. The four molecular markers are utilized for detecting whether Kasalath and derived varieties thereof contain the major gene locus, and consequently the rice stripe and leaf blight resistant level of the varieties can be predicted, and the selection efficiency of rice varieties with rice stripe and leaf blight resistance can be remarkably raised.

Description

1. Technical field [0001] The invention provides a molecular marker method for the main gene site qSTV11 resistant to rice stripe blight, belongs to the field of molecular genetics, and is specially used for the selection of rice stripe blight-resistant varieties and the screening and utilization of germplasm resources. 2. Background technology [0002] Rice stripe leaf blight is a viral disease caused by rice stripe virus, and its vector is mainly the small planthopper. In recent years, with the popularization and application of light cultivation techniques such as no-tillage, rice-wheat interleaving, and wheat-introducing rice, as well as susceptible high-yielding varieties, the expansion and harm of the SBPH carrier population of rice stripe leaf blight has been accelerated. The characteristics of short sucking time and long-lasting virus transmission of SBPH make pest control and disease prevention very difficult, and the occurrence of rice stripe leaf blight in my count...

AI Technical Summary

Problems solved by technology

The disease area in the severely diseased area accounts for more than 90% of the rice planting area. In the severely affected area, the rate of diseased holes and diseased plants in leaked fields is as high as 90%, and the rate of diseased plants is over 70%. Some fields basically lose harvest, causing huge economic losses

At present, the production is mainly combined with the adjustment of farming system and the improvement of cultivation technology, and the control of insects and diseases at the right time, but the effect is not good, and it pollutes the environment

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