59 results about "Genetic linkage analysis" patented technology

The invention provides a q SRBSDV6 (Southern rice black-streaked dwarf virus 6) and a molecular marker method thereof. BC3F7 is obtained through hybridization, backcross and selfing of Guangxi common wild rice material y11 and the susceptible variety Guanghui 998 and then is subjected to association analysis and genetic linkage analysis to obtain the q SRBSDV6, and the q SRBSDV6 is positioned between RM20148 and RM 20297; when the molecular marker RM20148 primer has q SRBSDV6, the amplification band is 129 bp band; when the molecular marker RM 20297 primer has q SRBSDV6, the amplification band is 172 bp band. According to the invention, the molecular markers of q SRBSDV6 genes can be adopted to detect whether the resistant material y11 and the derived varieties (series) contain the q SRBSDV6, the resistance level of the SRBSDV6 can be predicted, and the selective efficiency of the q SRBSDV6 rice can be greatly improved.

The invention provides molecular markers of genes which resist brown plant-hoppers of rice. The invention is characterized in that each offspring is obtained by hybridizing and back crossing a resistant parent RBPH54 and a susceptible variety TN1, and resistance identification and molecular genetic linkage analysis are carried out to the strain of each offspring, thus obtaining brown plant-hoppers resistant major recessive genes, bph20(t) and bph21(t); nearest molecular markers on both sides and linked with the bph20(t) are SSR markers, BYL7 and BYL8, which are autonomously developed, and the distances between the SSR markers and the bph20(t) are 1.3cm and 1.2cm respectively; and nearest molecular markers on both sides and linked with the bph21(t) are RM222 and RM224 and the distances between the RM222 and the bph21(t) and between the RM224 and the bph21(t) are 7.9cm and 4.0cm respectively. Whether the locus of the pest-resistant genes is contained in a pest-resistant variety RBPH54 and a derived variety (species) thereof can be detected by the molecular markers linked with the genes resistant to the brown plant-hoppers, which can greatly improve the selection efficiency of the brown plant-hopper resistant rice.

The invention relates to a molecular marking method of a major gene locus qSTV11 with rice stripe and leaf blight resistance and belongs to the field of molecular genetics. Hybridization with female plant of rice Nipponbare and male plant of Kasalath, backcross with Nipponbare and derivation are orderly realized, thus obtaining a backcross recombinant inbred system, genetic linkage analysis between genotypes and corresponding disease indexes and ratios in each family is realized, the resistant effects of the major gene locus qSTV11 with rice stripe and leaf blight resistance and allelic genes from Kasalath are detected, and molecular markers R21, R22, R13 and R48 that can be utilized in breeding are obtained through filtering. The four molecular markers are utilized for detecting whether Kasalath and derived varieties thereof contain the major gene locus, and consequently the rice stripe and leaf blight resistant level of the varieties can be predicted, and the selection efficiency of rice varieties with rice stripe and leaf blight resistance can be remarkably raised.

The present invention belongs to the molecular genetics field and relates to the the molecular marker method of the major gene loci conferring rice stripe disease resistance. The rice variety Nipponbare is hybridized with Kasalath and then backcrossed with Nipponbare. Via genetic linkage analysis of the genotype and disease index ratio of each family in aquired backcross inbred lines, the molecular marker BJ11-8 of the major rice stripe disease resistance gene loci qSTVll is obtained. Detection of this major gene locus in Kasalath and its derivatives via the molecular marker BJ11-8 will forecast the resistance level to the rice stripe disease and increase the selection efficiency of rice stripe disease resistant rices significantly.

The invention provides a rice brown planthopper resistant major gene Bph30 molecular marker and application thereof. A genotype of each F2 single plant obtained by hybridization of a rice insect-resistant variety AC1613 (male parent ) and a susceptible variety 9311 (female parent ) and a brown planthopper resistant phenotype of each F2 single plant is combined for genetic linkage analysis to obtain an insect-resistant variety AC1613 resistance gene Bph30 positioned between molecular markers RM16290 and RM16303, and molecular markers in linkage with the gene also include SSR-28, RM16294, SSR-68, SSR-69, RM16299, Indel-15, Indel-16 and SSR-35. By the molecular marker, whether a locus of the major gene exists in rice or not can be effectively detected, and accordingly brown planthopper resistant rice selection efficiency is greatly improved, and brown planthopper resistant rice varieties with the gene Bph30 can be obtained.

The invention relates to a rice black-streaked dwarf virus (RBSDV) resistant locus qRBSDV9 of a rice variety 9194 and a molecular marker method thereof. Four RBSDV resistant genetic loci of the RBSDV resistant variety 9194 are obtained by carrying out genetic linkage analysis on the genotype of a single plant F2 obtained by hybridizing the RBSDV resistant rice variety 9194 (male) with a susceptible variety Suyunuo (female) and the RBSDV resistance grade of a corresponding F2:3 family, wherein qRBSDV9 is between a marker RM3912 and a marker RM257. The RBSDV resistance levels of the resistant variety 9194 and derived varieties (lines) thereof can be predicated and the selection efficiency of the RBSDV resistant rice can be greatly improved by detecting whether the resistant variety 9194 and the derived varieties (lines) thereof contain the RBSDV resistance genetic loci via the molecular markers of the RBSDV resistant genes.

The invention relates to a molecular labeling of wheat fertility restorer genes Rf6 genes and its acquisition method, belonging to crop breeding and producing fields. Analyze the inheritance link of every single strain's gene-type and fertility thereof of the F2 generation group acquired by crossing wheat umbrella goatstraw transposition system N2114(íÔ) and perilla fruit (íß) to gain codominant SSR labeling MAG218 labeling and dominant labeling gwm88 labeling which are linked most tightly to T-type cytoplasm infertility restorer system Rf6. It could be used to make sure the existence of Rf6 and its existing state, and esmimate the fertility of wheat strains and futher screening check strains with Rf6 quickly to avoid the effection of environment to varieties which could increase the breeding velocity and identification efficiency of T-type cytoplasm infertility restorer system.

The invention discloses a molecular marker of a rice brown-plant-hopper-resistant gene Bph31 (t) and application of the molecular marker. Varieties K41 with high brown-plant-hopper resistance and varieties Gui1025 with high brown-plant-hopper sensitivity are hybridized to obtain F2:3 family population and recombinant selfing line population by the aid of heredity linkage analysis, the rice brown-plant-hopper-resistant gene Bph31 (t) on a fourth chromosome of rice is precisely positioned and is positioned in an interval between molecular markers MG81 and RM471, and the individual resistant plant selection efficiency of a single molecular marker MG96 in the interval is 97% approximately. The molecular marker which is in linkage with the gene further comprises the molecular markers MG81 and RM471. The molecular marker and the application have the advantages that the molecular marker can be used for detecting the insect-resistant varieties K41 and derived varieties (lines) of the insect-resistant varieties K41 to determine whether the insect-resistant varieties K41 and the derived varieties (lines) contain the gene or not, and accordingly brown-plant-hopper-resistant rice varieties with the brown-plant-hopper-resistant gene Bph31 (t) can be screened.

The invention provides a molecular marker of a brown planthopper resistance major gene qBph4(t) of paddy rice and an application of the molecular marker. Genotypes of all single strains of F2 are obtained by hybridization of insect-resistant varieties ARC5984 (male) and 9311 (female) of the paddy rice; genetic linkage analysis is performed according to a brown planthopper resistance level of an F2:3 family at the same time; the resistance major gene qBph4(t) carried by the insect-resistant variety ARC5984 is identified and located in a 6cM interval between molecular markers RM5635 and RM5742;and the resistance major gene qBph4(t) is tightly linked with the molecular marker RM5635. A molecular marker RM5757 is also linked with the gene. The molecular marker can effectively detect whether the insect-resistant variety ARC5984 and derived varieties contain a site of the resistance major gene; the selection efficiency of a brown planthopper resistance plant of the paddy rice is greatly improved; and the brown planthopper resistance variety of the paddy rice containing qBph4(t) is obtained.

The invention discloses a molecular marking method for tight interlocking of new gene Wbph 9 (t) for resisting rice sogatella furcifera, and application thereof. Through genetic linkage analysis, a variety F41 of the high resistant sogatella furcifera and a variety laurel 1025 of high sensitive sogatella furcifera to obtain F2 which consists of 3 family groups and recombinant inbred line groups; a rice resistant sogatella furcifera gene Wbph 9 (t) on the #6 chromosome of the rice is accurately positioned; the gene is positioned in a 120kb zone between the molecular marker MG11 and MG 32; the selecting efficiency of 1 molecular market MG21 of the zone for the antigen single plant is about 98%; the molecular market tightly interlocked by Wbph 9 (t) is applied to detect if the insect resisting variety K41 and its derivative variety (series) contains the gene; thus new material for resisting sogatella furcifera can be selected. The invention is applied to the molecular marking of the rice sogatella furcifera resistance so as to assist the seed breeding and pyramiding breeding, so as to perform the gene type selection on the low generation of breeding material in seedling period; thus the breeding efficiency is improved, and the breeding progress is accelerated.