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Molecular marker of major gene Bph6 resistant to brown planthopper and application thereof

A technology of anti-BPH and main gene, applied in the field of molecular genetics, can solve the problems of complex insect resistance identification, difficulty in effectively introducing and aggregating insect resistance genes, etc., and achieve the effects of convenient identification, shortened breeding cycle, and clear selection targets

Inactive Publication Date: 2010-12-15
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the complexity of insect resistance identification, it is often difficult to effectively introduce and aggregate different insect resistance genes using conventional breeding methods

Method used

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  • Molecular marker of major gene Bph6 resistant to brown planthopper and application thereof
  • Molecular marker of major gene Bph6 resistant to brown planthopper and application thereof
  • Molecular marker of major gene Bph6 resistant to brown planthopper and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) 9311 / Swarnalata F 2 Population construction and phenotyping

[0047] (1) In 1988, Kabir & Khush first identified the brown planthopper-resistant rice variety Swarnalata Bangladesh population using classical genetic analysis methods, and was controlled by a pair of dominant genes, so the brown planthopper-resistant gene was named Bph6 (Kabir & Khush.1988 Genetic analysis of resistance to brown planthopper in rice, Oryza sativa L. Plant Breeding 100, 54-58). The rice varieties come from the germplasm resource bank of the Chinese Academy of Agricultural Sciences, and Swarnalata comes from the rice germplasm resource bank of the International Rice Research Institute. Insect resistance identification experiments showed that Swarnalata has high resistance to the brown planthopper population in my country. In order to find simple and effective molecular markers that are closely linked to Bph6, the present invention uses the insect-susceptible variety 9311 as the female p...

Embodiment 2

[0072] Example 2 Verification of Molecular Markers

[0073] 1. Materials and methods

[0074] 1.1 Materials

[0075] Negative varieties: 10, susceptible varieties 9311, Nipponbare, Taichung Local No. 1, 7 non-resistant materials in the 9311XSwarnalata breeding combination.

[0076] Positive cultivars: 9 non-resistance materials in the breeding combination of the highly resistant cultivar Swarnalata and 9311 X Swarnalata.

[0077] Molecular marker primers: RM16994, Y19, Y9, RM119, RM5757, RM16999, RM17004, RM17008.

[0078] 1.2 Method

[0079] Genomic DNA of rice sample was extracted by CTAB extraction method (method is the same as in Example 1). Sample DNA was amplified with primers RM16994, Y19, Y9, RM119, RM5757, RM16999, RM17004, RM17008, respectively. The reaction system included 0.10 μM primers, 250 μM dNTPs, 1× PCR reaction buffer (50 mM KCl, 10 mM Tris-HCl pH8.3, 1.5 mM MgCl 2 ), 100ng of DNA template, 1UTaq enzyme. The reaction program was: 94°C pre-denaturation...

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Abstract

The invention provides a molecular marker of major gene Bph6 resistant to brown planthopper and an application thereof. The invention can obtain a pest-resistant cultivar Swarnalata resistance gene Bph6 and position the resistance gene between molecular marker Y1 and molecular marker Y9 by hybridizing a rice pest-resistant cultivar Swarnalata with 9311 (female) to obtain the genetype of each F2 plant and performing genetic linkage analysis by combining resistance levels resistant to brown planthopper of each family of F2:3. The molecular marker linked with the resistance gene is one of RM16994, Y19, Y9, RM119, RM5757, RM16999, RM17004 and RM17008. The molecular marker in the invention can effectively detect whether the major gene locus exists in the pest-resistant cultivar Swarnalata and derived varieties (strains) thereof, thus greatly improving the selection efficiency of rice resistant to brown planthopper and obtaining brown planthopper resistant rice varieties containing Bph6 gene.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a molecular marker of the main rice gene Bph6 resistant to brown planthopper, and also relates to the application of the molecular marker in breeding rice varieties resistant to brown planthopper. Background technique [0002] Rice is one of the main food crops in my country, but in recent decades rice has been damaged by a large number of diseases and insect pests, which seriously threatens rice production. Brown planthopper is a monophagous pest of rice. Its adults and nymphs use needles to suck rice juice, causing yellow leaves or withering, resulting in reduced yield or no harvest. According to the China Agricultural Yearbook, in 1966, 1969, 1973, 1977, 1983 and 2003, there were major outbreaks nationwide, and in 1987, 1991, 2005, 2006 and 2007, the damage area of ​​brown planthoppers reached more than 50% of the total area of ​​rice. It has caused serious losses to my country...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 何光存邱永福郭建平
Owner WUHAN UNIV
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