Anti-southern rice black-streaked dwarf disease site qSRBSDV9 and molecular marking method thereof

Abstract

The invention belongs to the field of plant molecular genetics and relates to an anti-southern rice black-streaked dwarf disease site qSRBSDV9 and a molecular marking method thereof. Through hybridization and selfing of a rice disease resistance line D4 and a susceptible variety Guanghui 998, a F2 group is obtained and is subjected to association analysis and genetic linkage analysis so that an anti-southern rice black-streaked dwarf disease site qSRBSDV9 is obtained and is located between molecular markers Indel32 and Indel35. Based on molecular marker Indel32 primers, when the qSRBSDV9 exists, the amplified band has 111 bp. Based on molecular marker Indel35 primers, when the qSRBSDV9 exists, the amplified band has 147 bp. Through the molecular markers of the anti-southern rice black-streaked dwarf disease gene, it is detected whether the resistant material D4 and its derivative varieties (lines) contain the anti-southern rice black-streaked dwarf disease site. The molecular marking method can predict the resistance level of the southern rice black-streaked dwarf disease and greatly improve the selection efficiency of rice against the southern rice black-streaked dwarf disease.

Description

technical field [0001] The invention belongs to the field of plant molecular genetics, and relates to a locus qSRBSDV9 resistant to southern rice black-streaked dwarf disease and a molecular marker method thereof. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the world's population uses rice as the main food. Since the black streak dwarf of southern rice was first discovered in 2001, the disease has spread rapidly in just a few years, causing great losses to the vast rice areas in southern my country and northern Vietnam. Different from common rice black-streaked dwarf disease, southern rice black-streaked dwarf disease is a rice virus disease mainly transmitted by white-backed planthopper, and its pathogenic virus is Southern rice black-streaked dwarf virus (Southern rice black- Streaked dwarf virus (SRBSDV for short) is a new species belonging to group 2 of the genus Fijivirus of the family Reoviridae. At p...

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Problems solved by technology

However, there are few reports on the genetic characteristics of resistance to black-streaked dwarf disease in southern rice, let alone the mapping of resistance genes / QTLs and breeding for resistance to black-streaked dwarf disease in southern rice.

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