Molecular marker of rice brown planthopper major gene qBph30(t) and application thereof

Abstract

The invention discloses a molecular marker of rice brown planthopper major gene qBph30(t) and an application thereof. In the invention, a close-linkage molecular marker of the resistant gene qBph30(t) in an insect-resistant strain 05BPH16 is obtained through a genotype of each single plant of the second filial generation obtained through hybridization between the rice insect-resistant strain 05BPH16 (male) and mosquito-resistant Qingzhan (female) with genetic linkage analysis with combination of anti-brown planthopper resistance levels in each family in F2:3. The molecular marker can effectively detect whether the resistant major gene exists in the insect-resistant strain 05BPH16 and derived varieties (or strains) or not and can quickly improve selective efficiency of an anti-brown planthopper rice material, thereby obtaining an anti-brown planthopper rice variety containing the qBph30(t) gene.

Description

technical field [0001] The invention belongs to the field of plant molecular genetics, and in particular relates to a molecular marker of rice rice main gene qBph30(t) resistant to brown planthopper and its application in breeding rice varieties resistant to brown planthopper. Background technique [0002] Rice brown planthopper (Nilaparvata lugens BPH for short) belongs to the Homoptera: Delphacidae insects, and is one of the most important insect pests in the rice planting process. It has piercing-sucking mouthparts, which directly pierce the phloem of the vascular bundles of the plant to absorb nutrients such as soluble sugars, inorganic salt ions, and amino acids in the juice, hindering the normal growth of rice plants. In severe cases, the whole plant will die and the grains will not be harvested. It is called "planthopper fire"; at the same time, the insect body acts as a medium for pathogens and spreads a variety of rice diseases, such as viral diseases, leaf dwarf ...

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Problems solved by technology

[0003] At present, the use of chemical insecticides to control the hazards of brown planthoppers is the most important control method in agricultural production. This method not only increases the cost of rice production, but also kills the natural enemies of brown planthoppers, which is likely to cause the outbreak of brown planthoppers again. Ecological factors such as pollution, as well as the accumulation of toxicity in the biological chain and corresponding human health problems; and the use of resistance genes of host plants to breed and promote the planting of brown planthopper-resistant rice varieties is the most economical, effective and environmentally friendly method for controlling brown planthopper

Starting from the early brown planthopper-resistant rice variety Mudgo, researchers have bred a number of brown planthopper-resistant varieties (lines), such as IR26, IR36, IR56 and IR64, etc. The promotion of these insect-resistant varieties has played a certain role in controlling the damage of brown planthopper; However, the large-scale promotion of brown planthopper-resistant rice varieties has caused the evolution of brown planthopper biotypes, and rice varieties carrying resistance genes will gradually lose their resistance to brown planthoppers.

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