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Molecular markers of rice stripe virus disease-resistant major gene locus qSTV11

A molecular marker and major gene technology, applied in the field of molecular genetics, can solve the problems of environmental pollution, economic loss, field loss, etc., and achieve the effect of not being affected by the environment, clear location, and convenient identification.

Inactive Publication Date: 2011-04-20
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

The disease area in the severely diseased area accounts for more than 90% of the rice planting area. In the severely affected area, the rate of diseased holes and diseased plants in leaked fields is as high as 90%, and the rate of diseased plants is over 70%. Some fields basically lose harvest, causing huge economic losses
At present, the production is mainly combined with the adjustment of farming system and the improvement of cultivation technology, and the control of insects and diseases at the right time, but the effect is not good, and it pollutes the environment

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  • Molecular markers of rice stripe virus disease-resistant major gene locus qSTV11
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Embodiment Construction

[0032] The detailed description of the present invention is as follows:

[0033] Studies have shown that there is a major gene locus that controls the stable expression of stripe leaf blight resistance at the same position on rice chromosome 11. Maeda H et al. (Maeda H, Nemoto H, Yagi T, Fukuta Y. QTL analysis for rice stripe disease resistance using recombinant inbred lines (RILs) derived from crossing between Milyang and Akihikari. In: China Association of Agricultural Science Societies, China National Rice Research Institute, China National Hybrid Rice Research and Development Center, China Foundation Society for Agricultural Science and Education (eds). Prospects of rice genetics and breeding for the 21st century-Paper collection of international rice genetics and breeding symposium. Beijing: China Agriculture Technology Press, 1999, 53~57.) Using the recombinant inbred line population of Miryang 23 / Qiuguang, it was detected that there was a QTL for resistance to stripe le...

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Abstract

The invention relates to molecular markers of a rice stripe virus disease-resistant major gene locus qSTV11, which belongs to the field of molecular genetics. The molecular markers are obtained by the following steps of: hybridizing a rice variety Nipponbare serving as a female parent with kasalath serving as a male parent; performing back crossing on a hybrid product and the Nipponbare; deriving to obtain a back crossing recombinant inbred line; performing genetic linkage analysis on the genotype of each family and corresponding disease index ratio; detecting the stripe virus disease-resistant major gene locus qSTV11, wherein alleles from kasalath have resistance effects; and screening to obtain molecular markers R21, R22, R13 and R48 which can be bred and utilized. The four molecular markers are used for detecting whether the kasalath and a derivative variety (line) contain the major gene locus or not, so that the rice stripe virus disease resistance level can be predicted and the selection efficiency of a stripe virus disease-resistant rice variety is improved remarkably.

Description

[0001] The present invention is a divisional application. The name of the original application is: Molecular marker method of qSTV11, the main gene locus for rice stripe leaf blight resistance. Application date: 2009.2.25 Application number: 200910024610.4. 1. Technical field [0002] The invention provides a molecular marker method for the main gene site qSTV11 resistant to rice stripe blight, belongs to the field of molecular genetics, and is specially used for the selection of rice stripe blight-resistant varieties and the screening and utilization of germplasm resources. 2. Background technology [0003] Rice stripe leaf blight is a viral disease caused by rice stripe virus, and its vector is mainly the small planthopper. In recent years, with the popularization and application of light cultivation techniques such as no-tillage, rice-wheat interleaving, and wheat-introducing rice, as well as susceptible high-yielding varieties, the expansion and harm of the SBPH carrier p...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 万建民江玲张迎信刘世家王宝祥王琦陈亮明刘喜
Owner NANJING AGRICULTURAL UNIVERSITY
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