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Main-gene bph2 molecular mark method for rice variety anti-brownspot gene site

A brown planthopper resistance and molecular marker technology, applied in the field of molecular genetics, can solve problems such as difficult aggregation of insect-resistant genes, and achieve the effects of convenient identification, clear location, and convenient and rapid detection.

Inactive Publication Date: 2007-01-17
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] Due to the complexity of insect resistance identification, it is often difficult to effectively aggregate different insect resistance genes using conventional breeding methods

Method used

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  • Main-gene bph2 molecular mark method for rice variety anti-brownspot gene site
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  • Main-gene bph2 molecular mark method for rice variety anti-brownspot gene site

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Embodiment Construction

[0030] The detailed description of the present invention is as follows:

[0031] Studies have shown that BPH resistance gene resources mainly exist in indica and wild rice species in Sri Lanka and India (Ikeda and Vaughau, 1991). Athwal et al. (1971) (Athwal D S, M D Pathak, E H Bacalangco, and C D Pura, Crop Sci.1971, 11: 747-750) reported that Mudgo, CO22 and MTU15 carry the same resistance gene Bph-1, and ASD7 carries a Recessive insect resistance gene bph-2. Athwal and Pathak (1972) reported that MGL2 contains the insect resistance gene Bph-1, and Ptb18 contains bph-2. Martinez and Khush (1974) reported that IR747B2-6 contained Bph-1 and that R1154-243 and IR4-93 contained bph-2. Lakshiminarayana and Khush (1977) reported that the Sri Lankan insect-resistant variety Rathu Heenati is controlled by a dominant gene Bph-3 that segregates independently from Bph-1; while the variety Babawee is controlled by a recessive gene bph-4 that segregates independently from bph-2 . Si...

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Abstract

The present invention belongs to the molecular genetics field and relates to the molecular marker method of the major gene bph2 for brown planthopper resistance in the rice variety ASD7. Via genetic linkage analysis of the individual F2 plant genotype and the brown planthopper resistance level of each F2:3 family produced by hybridizing the insect-resistant variety ASD7 with insect-susceptible variety C418, the molecular markers RM463 and RM7102 of the major gene loci bph2 in the insect-resistant variety ASD7 are obtained. In the ASD7 / C418BC1 population the correct rate of selection by either marker is 91.2%. In the BC2 population, the correct rate of selection by RM463 is 91.2%, the correct rate of selection by RM7102 is 89.9%. Detection of this major gene loci in ASD7 and its derivatives via these molecular markers will increase the selection efficiency of brown planthopper resistant rices.

Description

1. Technical field [0001] The invention provides a molecular marker method of rice variety ASD7 resistant to brown planthopper main gene bph2, belongs to the field of molecular genetics, and is specially used for the breeding of rice varieties resistant to brown planthopper and the utilization of germplasm resources. 2. Technical background [0002] Rice is an important food crop in my country. The brown planthopper is a monophagous pest of rice, belonging to the family Plantagoidae of the order Homoptera. It is a typical piercing-sucking pest that sucks the phloem sap of rice stems through the stylet. In severe cases, the lower part of the rice plant will turn black, rotten and smelly, and paralyzed, which is called "lice burning", resulting in reduced or lost rice production. At present, the control of brown planthopper mainly depends on chemical pesticides, but this measure not only increases the production cost, but also pollutes the environment. Therefore, the most ec...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 万建民江玲孙立宏刘裕强刘喜陈亮明刘世家王春明程遐年翟虎渠
Owner NANJING AGRICULTURAL UNIVERSITY
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