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Wheat fertile activity recovery gene RF6 molecular mark and its obtaining method

A fertility restorer gene and molecular marker technology, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve the problem of indetermination of homozygous state and heterozygous state of restorer genes, no SSR markers found, no close linkage and other problems, to achieve the effect of improving the efficiency of selection and identification, convenient identification, and accelerated utilization

Inactive Publication Date: 2005-01-26
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

At the same time, it was reported that no closely linked SSR markers were found, and it was predicted that the map was not saturated
Currently, molecular markers linked to Rf6 include RFLP, RAPD and ISSR, all of which are dominant markers, that is, it cannot be determined whether the restorer gene exists in a homozygous state or a heterozygous state
No closely linked codominant SSR markers have been reported

Method used

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  • Wheat fertile activity recovery gene RF6 molecular mark and its obtaining method
  • Wheat fertile activity recovery gene RF6 molecular mark and its obtaining method
  • Wheat fertile activity recovery gene RF6 molecular mark and its obtaining method

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Embodiment Construction

[0041] N2114 is the 5th self-bred offspring of 2114. After test cross identification, it is a translocation line of A. microcapsola carrying the Rf6 gene located on the translocation fragment of the 6U chromosome of A. microcapsatus. Its cytoplasm is T-type cytoplasm, so , the fertility of the translocation line directly reflects the ability of Rf6 to restore fertility. By crossing the donor parent N2114 of Rf6 with the wheat variety or recipient parent, self-crossing or backcrossing the offspring, and using molecular markers MAG218 marker and gwm88 marker to select a single plant containing Rf6, it is possible to breed a recovery Restorers of male sterility with gene Rf6 but with different genetic backgrounds. In the breeding process of restorer lines, due to the use of molecular markers, there is no special requirement for the cytoplasm of the female parent in the cross. In traditional breeding methods, if the cytoplasm is derived from common wheat, it is difficult for us t...

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Abstract

The invention relates to wheat fertility recovery gene Rf6 molecular tag and its obtaining method and belongs to crop breeding and production fields. F2 population arising from wheat T.um-bellulatum translocation line N2114 with susan is analysed in individual genotype and its fertility. Codominant SSR mark MAG218 and dominant mark gwm88 is obtained, which are closely linked to T cell cytoplasm sterility fertility recovery gene Rf6, and can determine the existence of Rf6 and forecast the wheat plant fertility, so can screening the Rf6 plant rapidly.

Description

1. Technical field [0001] The molecular marker of the wheat fertility restorer gene Rf6 and the method for obtaining the same provide a co-dominant molecular marker closely linked with the T-type wheat cytoplasmic male sterility restorer gene Rf6, which belongs to the field of crop breeding. It is specially used for the establishment, breeding and identification of T-type wheat male sterility restorer line germplasm. It can also be used for cloning restorer genes and transgenic breeding. 2. Technical Background [0002] Cytoplasmic male sterility (CMS) is a maternally inherited inability to produce normal functional pollen but the female reproductive organs are unaffected. The fertility restorer gene Rf located in the nucleus can restore its fertility. Studies have shown that fertility restoration genes are generally dominant. CMS and its fertility restoration system play an important role in the production of crop hybrids. On the one hand, it eliminates the cumbersomene...

Claims

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Application Information

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IPC IPC(8): A01H1/04C07H21/04C12N15/11C12N15/29C12Q1/68
Inventor 马正强杨郁文
Owner NANJING AGRICULTURAL UNIVERSITY
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