39results about How to "Improve selection identification efficiency" patented technology

The invention belongs to the technical field of molecular breeding, and provides an SNP molecular marker linked with a wheat stripe rust resistance gene QYr.sicau-1B.1 and application thereof. The SNPmolecular marker is KASP-Yr, which can be obtained by amplifying a primer having a nucleotide sequence as shown in SEQ ID NO.1-3. Detection analysis indicates that the molecular marker can be used for accurately tracking wheat stripe rust resistant QTL QYr.sicau-1B.1 and predicting the stripe rust resistance characteristics of wheat, so that molecular designed breeding can be performance conveniently. The invention further discloses a method for identifying the molecular marker linked with wheat stripe rust resistance QTL QYr.sicau-1B.1. By utilizing the method, the stripe rust resistance prediction accuracy can be reinforced to quickly screen wheat variety or strain with stripe rust resistance for breeding, the breeding process for high-yield variety of wheat can be greatly accelerated.

The invention discloses a wheat spikelet number QTL linked SNP molecular marker and application thereof. The SNP molecular marker KASP-1 is located on the short arm of a 2D chromosome of the RefSeqv1.0 genome version, and the 100 bp sequence before and after is shown as SEQ ID NO.46, the polymorphism is C / T and can be obtained by primer amplification shown in SEQ ID NO.1-3. The molecular marker can accurately track the wheat spikelet number QTL QSns.sau-2D.1 to predict the wheat spikelet number and facilitate molecular breeding. The invention also discloses a method for identifying the wheat spikelet number QTL QSns.sau-2D.1 molecular marker. By means of the method, the wheat spikelet number prediction accuracy can be enhanced, so as to quickly screen out wheat varieties or lines with increased spikelet number for breeding, and the breeding process of the wheat high yield varieties can be accelerated.

The invention relates to the field of triticum aestivum L. molecular breeding, and particularly discloses a triticum aestivum L. flag leaf length quantitative trait locus (QTL) linked molecular markerand application thereof. The single nucleotide polymorphism (SNP) molecular marker linked with triticum aestivum L. flag leaf length QTL QFll-5B is provided and located on a 5B chromosome long arm ofa RefSeqv1.0 genome edition, and is the 51st of a sequence shown in SEQ ID NO.31, polymorphism is C / T, and the SNP molecular marker can be obtained by amplification of a primer shown in a nucleotidesequence such as SEQ ID NO.1-3. The SNP molecular marker can accurately trace the triticum aestivum L. flag leaf length QTL QFll-5B, and predict the flag leaf length characteristics of triticum aestivum L., and molecular design breeding is convenient. The invention further discloses a method for identifying the molecular marker. The prediction accuracy of the flag leaf length can be enhanced by using the method, so that varieties or strains of the triticum aestivum L. with increased the flag leaf length are conveniently and rapidly screened for breeding, and the breeding progress of the varieties of the triticum aestivum L. with a high yield can be greatly accelerated.

The invention belongs to the field of crop breeding, and relates to a molecular marker of wheat scab resistance expansion gene Fhbl and application thereof. The molecular marker is one of MAG6067 and MAG6660 with a genetic distance to an Fhbl gene being respectively 0.05cM and 0cM. A molecular marker which is closest linked to the Fhbl for the first time internationally by utilizing the invention. The Fhbl gene can be detected by marking the MAG6660 to determine whether the Fhbl exists or not and the existing state of the Fhbl, and the scab resistance of wheat can be predicted, so that a plant carrying the Fhbl can be quickly screened; and the molecular marker can be used for breeding a scab-resistance variety. Furthermore, laboratory detection can be carried out by utilizing the molecular marker to avoid the influence of the environment to the phenotype. According to the molecular marker closely linked with the Fhbl, time and labor for screening scab resistance materials can be greatly saved, the prediction accuracy can be enhanced, and clone of the Fhbl gene can be accelerated.

The invention discloses a molecular marker SSR52 which is closely linked with wheat few-tillering gene Ltn3, wherein the nucleotide sequence of the molecular marker SSR52 is represented as the SEQ ID No.1. Genetic distance between the molecular marker and the wheat few-tillering gene Ltn3 is 0.3 cM. A test result proves that the molecular marker can accurately trace the wheat few-tillering gene and predict tillering characters of wheat, thereby further carrying molecular-designing breeding conveniently. The invention also discloses a method of identifying the molecular marker of the wheat few-tillering gene Ltn3. By means of the method, accuracy of tillering prediction can be increased, success rate of specific plant type breeding can be increased, and achievement of an object of increasing per unit area yield of wheat is accelerated.

The invention relates to a molecular labeling of wheat fertility restorer genes Rf6 genes and its acquisition method, belonging to crop breeding and producing fields. Analyze the inheritance link of every single strain's gene-type and fertility thereof of the F2 generation group acquired by crossing wheat umbrella goatstraw transposition system N2114(íÔ) and perilla fruit (íß) to gain codominant SSR labeling MAG218 labeling and dominant labeling gwm88 labeling which are linked most tightly to T-type cytoplasm infertility restorer system Rf6. It could be used to make sure the existence of Rf6 and its existing state, and esmimate the fertility of wheat strains and futher screening check strains with Rf6 quickly to avoid the effection of environment to varieties which could increase the breeding velocity and identification efficiency of T-type cytoplasm infertility restorer system.

The invention provides a molecular marker for increasing barley harvest index QTL site under low phosphor condition and an application thereof. The molecular marker is Hvc316, a nucleotide sequence isshown as SEQ ID No:1, the molecular marker is positioned on barley 3H chromosome, and can accurately track the site for increasing a barley harvest index QTL under low phosphor condition, predicts the harvest index characteristic of the barley under the low phosphor condition, and is convenient for molecular design breeding with nutrient efficient utilization. The invention also provides the application of the molecular marker Hvc316 in barley high-yield nutrient efficient breeding. A method provided in the invention is capable of enhancing the accuracy of prediction of the barley harvest index under low phosphor condition, and is convenient for rapidly screening the barley variety or lines used for breeding for increasing the barley harvest index QTL under low phosphor condition, so thata seed selection process of the barley high-yield high-efficiency variety can be greatly accelerated.

The invention discloses a molecular marker for efficient utilization of a QTL site by barley phosphorus and an application of the marker. The molecular marker is Hvc630, a nucleotide sequence of the marker is shown in SEQ ID NO: 1 and is positioned on a barley 3H chromosome, and thus the marker can accurately track the QTL site efficiently utilized by the barley phosphorus, predict barley phosphorus utilization efficiency characteristics, and further facilitate molecular design breeding for nutrient efficient utilization. The invention further provides the application of the molecular marker Hvc630 in barley breeding with high-yield high-efficiency nutrients. By using the method, the accuracy of barley phosphorus utilization efficiency prediction can be enhanced, and barley varieties or strains with QTL for improving barley phosphorus utilization efficiency can be rapidly screened for breeding, so that the breeding process of high-yield high-efficiency barley varieties can be greatly accelerated.

The invention provides a molecular marker closely linked to the wheat grain filling rate QTL QGfr.sicau-6D. The molecular marker is K603, and the nucleotide sequence of the molecular marker is as shown by SEQ ID NO.1. The molecular marker K603 is closely linked to the wheat grain filling rate QTL. Detection analysis shows that the molecular marker can be used for accurately tracking the wheat grain filling rate QTL and predicting the wheat grain filling rate characteristics to further provide convenience for molecular design breeding. Through detection, the molecular marker K603 can enhance the accuracy of the wheat grain filling rate prediction, so that the wheat varieties or strains with the grain filling rate increasing QTL can be fast screened for breeding, and the breeding progress ofthe wheat high-yield varieties can be greatly accelerated.

The invention relates to the field of barley molecular breeding, and in particular discloses a molecular marker HRM1 of barley grain length gene LkI1 and application of the molecular marker. The nucleotide sequence of the molecular marker HRM1, which is closely linked with the barley grain length gene LkI1, is as shown in SEQ ID No.1. Upon detection and analysis, the molecular marker can accurately track the barley grain length gene and predict the grain length traits of the barley, so as to facilitate molecular designing and breeding. The invention also discloses a method for identifying the molecular marker of the barley grain length gene LkI1; by virtue of the method provided by the invention, the accuracy of grain length prediction is enhanced, so that barley varieties or lines having enhanced grain length QTL can be conveniently and rapidly screened out; and the process of selecting high-yield barley varieties can be greatly accelerated.

The invention relates to a molecular marker of a wheat scab infection -resistant gene Fhb5 and an application thereof, belonging to the field of crop breeding. The molecular marker is either MAG8217 or MAG2462, whose genetic distance to the Fhb5 gene is 0.02 cM or 0 cM respectively. In the world, a molecular marker which is linked to the Fhb5 closestly is first acquired in the invention. Either marker is a codominant marker, which has advantages of being convenient in detection, stable for amplification, and simple in operation. The markers of the MAG8217 and the MAG2462 are used to detect Fhb5 gene, so that existence and the existing state of Fhb5 can be determined and the scab resistance of wheat can be predicted, thereby rapidly screening Fhb5-containing plants and using the plants for seed selection of disease-resistant varieties. At the same time, the molecular marker can be used in laboratory detection to avoid environmental influences on phenotypes. In the invention, using the molecular marker tightly linked to the Fhb5 can substantially save time and labor for screening disease-resistant materials, improve the prediction accuracy, and accelerate the cloning of the Fhb5 gene.

The invention relates to the field of genetic breeding, and particularly relates to SNP molecular markers linked with rice sodium and potassium ion absorption QTL qK / Na-3-1, primers and application. The SNP molecular markers RS (36172492) and RS (36174628) are located on a Chr3 chromosome short arm of an IRGSP-1.0 genome version, the sequences are shown as SEQ ID NO. 1 and SEQ ID NO. 2, the polymorphisms are (T / G) (G / -), (A / -), and the SNP molecular markers can be obtained by amplifying the primers shown as SEQ ID NO. 3 and SEQ ID NO. 5. The molecular markers can accurately track the rice sodium and potassium ion absorption QTL qK / Na-3-1 and predict the potential salt tolerance characteristic of rice, and then molecular breeding is facilitated. The invention further discloses a method foridentifying the rice sodium and potassium ion absorption QTL qK / Na-3-1 molecular markers. By means of the method, the accuracy of sodium and potassium ion absorption prediction can be enhanced, so that rice varieties or strains with potential enhanced rice salt tolerance are quickly screened out for breeding, and the breeding process of the rice varieties with potential salt tolerance can be greatly accelerated.

The invention discloses a molecular marker HRM5 closely linked with the wheat oligo-tiller gene Ltn3, the nucleotide sequence of which is shown in SEQ ID No.1, and the genetic distance between the molecular marker and the wheat oligo-tiller gene Ltn3 is 0.35cM. Detection and analysis show that the molecular marker can accurately track the wheat oligo-tiller gene, predict the tiller characteristics of wheat, and facilitate molecular design breeding. The invention also discloses a method for identifying the molecular marker of the wheat oligo-tiller gene Ltn3. The method provided by the invention can enhance the accuracy of tiller prediction, improve the success rate of specific plant type breeding, and accelerate the realization of the goal of increasing wheat yield per unit area .

The invention discloses a wheat maximum root length QTLQMrl.sau-3D linked SNP (Single Nucleotide Polymorphism) molecular marker and application thereof, and relates to the field of wheat molecular genetic breeding. The SNP molecular marker and QMrl.sau-3D are co-localized on a 3D chromosome long arm of a wheat genome, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.16, and C / T mutation exists at the 36bp position of the SNP molecular marker. The SNP molecular marker KASP-Mrl-sau1 is closely linked with the maximum root length QTLQMrl.sau-3D of wheat, the maximum root length QTLQMrl.sau-3D of wheat can be accurately tracked, the maximum root length characteristic of wheat can be predicted, the selection and identification efficiency of the maximum root length of wheat can be remarkably improved, wheat varieties or strains with the long root length can be rapidly screened out, and the application prospect is wide. The process of wheat molecular genetic breeding is accelerated.

The invention belongs to the field of crop breeding, and discloses molecular markers of a wheat scab infection resistance gene Fhb4 and application of the molecular markers. The molecular markers are selected from any one of MAG5184, MAG7237 and MAG2521, and the genetic distances to Fhb4 gene are 0.72cM, 0.34cM and 0.92cM, respectively. According to the invention, the molecular markers having the closest linkage with Fhb4 are obtained for the first time in the world. These three molecular markers are codominant and have the advantages of convenient detection, and stable, simple and convenient amplification. The molecular marker MAG7237 is used to detect Fhb4 gene to determine the existence and status of Fhb4 and predict the scab resistance of wheat, and then to screen the plants with Fhb4 gene, which are used for breeding disease-resistant varieties.

The invention discloses a KASP molecular marker linked to wheat flag leaf length QTL QFll-2B and application thereof. The molecular marker is located on the long arm of chromosome 2B of a RefSeqv1.0 genome. The detection and analysis show that the molecular marker can accurately track the wheat flag leaf length QTL QFll-2B, predict wheat flag leaf length characteristics, and then facilitate molecular breeding. The invention also discloses a method for identifying the molecular marker of the wheat flag leaf length QTL QFll-2B, and the method provided by the invention can be used to enhance theaccuracy of predicting the flag leaf length, and can screen wheat varieties or lines with longer flag leaves more quickly for wheat breeding, greatly accelerating the process of wheat breeding.

The invention belongs to the technical field of molecular breeding, and provides a SNP molecular marker linked with the wheat stripe rust resistance gene QYr. The primers indicated by ID NO.1-3 were amplified. Detection and analysis show that the molecular marker can accurately track the wheat stripe rust resistance QTL QYr.sicau‑1B.1, predict the stripe rust resistance characteristics of wheat, and facilitate molecular design breeding. The invention also discloses a method for identifying molecular markers of wheat stripe rust resistance QTL QYr. The use of stripe rust wheat varieties or lines for breeding can greatly speed up the process of breeding high-yield varieties of wheat.

The invention relates to the field of wheat molecular breeding, and specifically discloses an SNP molecular marker linked to an effective tiller number QTL QPTN. Sau-4B of wheat and application thereof. The molecular marker is KASP-1, and can be obtained by amplification of primers of which nucleotide sequences are shown in SEQ ID NO. 1-3. Detection analysis shows that the molecular marker can beused for accurately tracking the effective tiller number QTL QPTN. Sau-4B of the wheat, predicting the effective tiller number characteristics of the wheat, and further facilitating molecular design breeding. The invention also discloses a molecular marker method for identifying the effective tiller number QTL QPTN. Sau-4B of the wheat, and by adopting the method, the accuracy of prediction of theeffective tiller number can be improved so as to quickly screen out wheat varieties or lines with increased effective tiller number for breeding, and the breeding process of high-yield varieties of the wheat can be greatly speeded up.

The invention discloses a SNP molecular marker KASP-BE-kl-sau2 linked with the main effect QTL of wheat grain length and its application, belonging to the field of crop molecular genetics and breeding. The molecular marker KASP‑BE‑kl‑sau2 polymorphism is C / T, KASP‑BE‑kl‑sau2 co-localizes with wheat grain length QTL Qkl.sau‑BE‑4A on the short arm of wheat chromosome 4A, and is located Within the Qkl.sau‑BE‑4A interval. The molecular marker KASP-BE-kl-sau2 disclosed by the present invention is extremely significantly correlated with the grain length QTLQkl.sau-BE-4A, presents the characteristics of closely linked markers, has high accuracy for molecular marker-assisted selection, and can accurately predict wheat grain length Traits play an important role in improving wheat yield and efficiently screening high-quality wheat varieties with longer grain length, and are also conducive to improving the efficiency of wheat breeding.

The invention belongs to the field of breeding of plants and discloses a molecular marker MAG7237 of a wheat fusarium head blight infection resistant gene Fhb4 and application of the molecular marker MAG7237. The genetic distance between the molecular marker MAG7237 and the Fhb4 gene is 0.34 cm. The molecular marker most closely linked with the Fhb4 is acquired by the invention for the first time in the world. The molecular marker is a co-dominant marker with the advantages of being convenient to detect, and stable and simple to amplify; by adopting the marker MAG7237 to detect the Fhb4 gene, the existence of the Fhb4 or not and the existence state can be determined, and the fusarium head blight resistance of the wheat can be forecasted; and furthermore, the plants with the Fhb4 can be fast screened and used for breeding of disease-resistant varieties.

The invention discloses a wheat tiller angle QTL QTA‑2B linked molecular marker and its application. The KASP‑sicau1 molecular marker is a SNP molecular marker, and the polymorphism is A / G. It co-localizes with wheat tiller angle QTL QTA‑2B on the long arm of wheat chromosome 2B, and is located in the QTL QTA‑2B interval. The molecular marker KASP-sicau1 disclosed in the present invention is extremely significantly correlated with the tiller angle QTL QTA-2B, presents the characteristics of closely linked markers, has high accuracy for molecular marker-assisted selection, and can significantly improve wheat varieties with larger tiller angles adapted to different environments The selection and identification efficiency is high, and the success rate is high.

The invention belongs to the technical field of crop molecular genetic breeding, and discloses a KASP molecular marker linked with wheat stripe rust resistance QTL and application. The nucleotide sequence of the KASP molecular marker comprises a first nucleotide sequence and a second nucleotide sequence; and the first nucleotide sequence is SEQ ID NO: 1, and the second nucleotide sequence is SEQ ID NO: 2. The molecular marker linked with wheat stripe rust resistance QTL and located on a wheat 2A chromosome is a flanking marker of a stripe rust resistance site QYr.sicau-2AS on a short arm of the wheat 2A chromosome, and the linkage degree is high. The marker can be used for detecting the stripe rust resistance site QYr.sicau-2AS on the wheat 2A chromosome, plants with the loci are rapidly screened, and then the wheat disease resistance breeding efficiency is improved.

The invention provides a molecular marker for increasing barley harvest index QTL site under low phosphor condition and an application thereof. The molecular marker is Hvc316, a nucleotide sequence isshown as SEQ ID No:1, the molecular marker is positioned on barley 3H chromosome, and can accurately track the site for increasing a barley harvest index QTL under low phosphor condition, predicts the harvest index characteristic of the barley under the low phosphor condition, and is convenient for molecular design breeding with nutrient efficient utilization. The invention also provides the application of the molecular marker Hvc316 in barley high-yield nutrient efficient breeding. A method provided in the invention is capable of enhancing the accuracy of prediction of the barley harvest index under low phosphor condition, and is convenient for rapidly screening the barley variety or lines used for breeding for increasing the barley harvest index QTL under low phosphor condition, so thata seed selection process of the barley high-yield high-efficiency variety can be greatly accelerated.

The invention provides a molecular marker closely linked to the wheat grain filling rate QTL QGfr.sicau-7D.1, the molecular marker is KASP705, and its nucleotide sequence is shown in SEQ ID NO. 1; the molecular marker KASP705 is associated with the wheat grain The filling rate QTLs are tightly linked. Detection and analysis showed that the molecular marker could accurately track the QTL of the grain filling rate of wheat, predict the grain filling rate characteristics of wheat, and then facilitate molecular design breeding. The detection molecular marker KASP705 can enhance the accuracy of wheat grain filling rate prediction, so as to quickly screen wheat varieties or lines with QTL increasing grain filling rate for breeding, which can greatly speed up the breeding process of high-yielding wheat varieties.

The invention discloses wheat grain weight major gene locus QGw.nau-4B molecular marker primers and application thereof. In the wheat grain weight major gene locus QGw.nau-4B molecular marker primers, the sequence of the molecular marker primer WGRC1334-F is as shown in SEQ ID NO.1 and the sequence of WGRC1334-R is as shown in SEQ ID NO.2; the sequence of the molecular marker primer WGRC1083-F is as shown in SEQ ID NO.3 and the sequence of the WGRC1083-R is as shown in SEQ ID NO.4. The WGRC1334 and WGRC1083 marked primers are used for detecting a QGW.nau-4B gene, the existence or nonexistence and the existence state of the QGW.nau-4B can be determined, and the grain weight characteristic of the wheat can be predicted, so that plants with the QGw.nau-4B can be quickly screened and the molecular marker primers can be used for improving the wheat variety.

The invention discloses a KASP-Flw-sau6198 molecular marker linked with the main effect QTL of wheat flag leaf width and its application, belonging to the fields of molecular biology and crop genetics and breeding. The molecular marker is a SNP molecular marker, and the polymorphism is A / G, which co-localizes with the wheat flag leaf width QTL QFLW.sau-AM-4B.4 on the long arm of the tetraploid wheat chromosome 4B, and is located in the QTL QFLW .sau‑AM‑4B.4 range. After detection and analysis, it is shown that the molecular marker can accurately track the flag leaf width QTLQFLW.sau‑AM‑4B.4 of wheat, and predict the flag leaf width characteristics of wheat, which is conducive to more rapid screening of wheat varieties or lines with wider flag leaves For breeding, greatly speed up the process of wheat breeding.