39results about How to "Improve selection identification efficiency" patented technology

The invention discloses a molecular marker SSR52 closely linked with the wheat oligo-tiller gene Ltn3, the nucleotide sequence of which is shown in SEQ ID No.1, and the genetic distance between the molecular marker and the wheat oligo-tiller gene Ltn3 is 0.3cM. Detection and analysis show that the molecular marker can accurately track the wheat oligo-tiller gene, predict the tiller characteristics of wheat, and facilitate molecular design breeding. The invention also discloses a method for identifying the molecular marker of the wheat oligo-tiller gene Ltn3. The method provided by the invention can enhance the accuracy of tiller prediction, improve the success rate of specific plant type breeding, and accelerate the realization of the goal of increasing wheat yield per unit area .

The invention discloses a molecular marker HRM5 closely linked with the wheat oligo-tiller gene Ltn3, the nucleotide sequence of which is shown in SEQ ID No.1, and the genetic distance between the molecular marker and the wheat oligo-tiller gene Ltn3 is 0.35cM. Detection and analysis show that the molecular marker can accurately track the wheat oligo-tiller gene, predict the tiller characteristics of wheat, and facilitate molecular design breeding. The invention also discloses a method for identifying the molecular marker of the wheat oligo-tiller gene Ltn3. The method provided by the invention can enhance the accuracy of tiller prediction, improve the success rate of specific plant type breeding, and accelerate the realization of the goal of increasing wheat yield per unit area .

The invention discloses a KASP molecular marker linked with wheat flag leaf long QTL QFll‑2B and an application thereof. The molecular marker is located on the long arm of chromosome 2B of the RefSeqv1.0 genome. Detection and analysis show that the molecular marker can accurately track the wheat flag leaf length QTL QFll-2B, predict the flag leaf length characteristics of wheat, and facilitate molecular breeding. The invention also discloses a method for identifying the molecular marker of the wheat flag leaf length QTL QFll-2B. The method provided by the invention can enhance the accuracy of the prediction of the flag leaf length, and can more quickly screen out those with longer flag leaves. Wheat varieties or strains are used for breeding, which greatly speeds up the process of wheat breeding.

The invention discloses a SNP molecular marker linked with wheat grain length QTL and its application, belonging to the field of crop molecular genetics and breeding; the SNP molecular marker is KASP-KL-sau1, and the polymorphism is C / T; the KASP-KL ‑sau1 co-localizes with the wheat grain length QTL QKL.sau‑1B on the long arm of the wheat 1B chromosome, and the genetic distance between it and the QTL QKL.sau‑1B is 4 cM, and the nucleotide sequence of 35bp before and after it is shown in SEQ ID NO: 31. The KASP-KL-sau1 disclosed in the present invention is extremely significantly correlated with the grain length QTL QKL.sau-1B, presents the characteristics of closely linked markers, has high accuracy for molecular marker-assisted selection, and can significantly improve the long-grain varieties of wheat. The selection and identification efficiency is high, and the success rate is high. While improving the efficiency of breeding work, it provides a basis for the study of wheat grain length genes.

The invention relates to the field of wheat molecular breeding, and specifically discloses an SNP molecular marker linked to an effective tiller number QTL QPTN. Sau-4B of wheat and application thereof. The molecular marker is KASP-1, and can be obtained by amplification of primers of which nucleotide sequences are shown in SEQ ID NO. 1-3. Detection analysis shows that the molecular marker can beused for accurately tracking the effective tiller number QTL QPTN. Sau-4B of the wheat, predicting the effective tiller number characteristics of the wheat, and further facilitating molecular design breeding. The invention also discloses a molecular marker method for identifying the effective tiller number QTL QPTN. Sau-4B of the wheat, and by adopting the method, the accuracy of prediction of theeffective tiller number can be improved so as to quickly screen out wheat varieties or lines with increased effective tiller number for breeding, and the breeding process of high-yield varieties of the wheat can be greatly speeded up.

The present invention provides a molecular marker closely linked with the wheat grain filling rate QTL QGfr.sicau-6D, the molecular marker is K603, and its nucleotide sequence is shown in SEQ ID NO. 1; the molecular marker K603 is closely related to the wheat grain filling rate The QTLs are tightly linked. Detection and analysis showed that the molecular marker could accurately track the QTL of the grain filling rate of wheat, predict the grain filling rate characteristics of wheat, and then facilitate molecular design breeding. The detection molecular marker K603 can enhance the accuracy of wheat grain filling rate prediction, so that wheat varieties or lines with QTLs that increase grain filling rate can be quickly screened for breeding, which can greatly speed up the breeding process of high-yielding wheat varieties.

The invention provides a molecular marker for increasing barley harvest index QTL site under low phosphor condition and an application thereof. The molecular marker is Hvc316, a nucleotide sequence isshown as SEQ ID No:1, the molecular marker is positioned on barley 3H chromosome, and can accurately track the site for increasing a barley harvest index QTL under low phosphor condition, predicts the harvest index characteristic of the barley under the low phosphor condition, and is convenient for molecular design breeding with nutrient efficient utilization. The invention also provides the application of the molecular marker Hvc316 in barley high-yield nutrient efficient breeding. A method provided in the invention is capable of enhancing the accuracy of prediction of the barley harvest index under low phosphor condition, and is convenient for rapidly screening the barley variety or lines used for breeding for increasing the barley harvest index QTL under low phosphor condition, so thata seed selection process of the barley high-yield high-efficiency variety can be greatly accelerated.

The invention discloses wheat grain weight major gene locus QGw.nau-4B molecular marker primers and application thereof. In the wheat grain weight major gene locus QGw.nau-4B molecular marker primers, the sequence of the molecular marker primer WGRC1334-F is as shown in SEQ ID NO.1 and the sequence of WGRC1334-R is as shown in SEQ ID NO.2; the sequence of the molecular marker primer WGRC1083-F is as shown in SEQ ID NO.3 and the sequence of the WGRC1083-R is as shown in SEQ ID NO.4. The WGRC1334 and WGRC1083 marked primers are used for detecting a QGW.nau-4B gene, the existence or nonexistence and the existence state of the QGW.nau-4B can be determined, and the grain weight characteristic of the wheat can be predicted, so that plants with the QGw.nau-4B can be quickly screened and the molecular marker primers can be used for improving the wheat variety.

The invention discloses a KASP-Flw-sau6198 molecular marker linked with the main effect QTL of wheat flag leaf width and its application, belonging to the fields of molecular biology and crop genetics and breeding. The molecular marker is a SNP molecular marker, and the polymorphism is A / G, which co-localizes with the wheat flag leaf width QTL QFLW.sau-AM-4B.4 on the long arm of the tetraploid wheat chromosome 4B, and is located in the QTL QFLW .sau‑AM‑4B.4 range. After detection and analysis, it is shown that the molecular marker can accurately track the flag leaf width QTLQFLW.sau‑AM‑4B.4 of wheat, and predict the flag leaf width characteristics of wheat, which is conducive to more rapid screening of wheat varieties or lines with wider flag leaves For breeding, greatly speed up the process of wheat breeding.