Molecular marker of wheat scab resistance expansion gene Fhbl and application thereof
A technology of molecular markers and extended genes, which is applied in the determination/testing of microorganisms, DNA preparation, DNA/RNA fragments, etc., can solve the problem of poor agronomic traits of scab-resistant germplasm, time-consuming and labor-intensive resistant varieties, and susceptibility to environmental influences and other problems, to achieve the effect of improving the efficiency of selection and identification, convenient detection, and convenient identification
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[0039] The above-mentioned molecular marker of the wheat head blight resistance extended gene Fhb1 was obtained by the following method:
[0040] (1) Wangshuibai Fhb1 near-isogenic line NMAS016 and its recurrent parent Mianyang 99-323F 2:3 Creation of populations and screening of Fhb1 segment recombinants:
[0041] (1) NMAS016(♀) and wheat variety Mianyang 99-323 hybrid F 1 , F 1 Selfing produced F containing 2875 individuals 2 group;
[0042] (2) Using the boundary markers Xbarc147 and Xgwm493 of Fhb1 (http: / / wheat.pw.usda.gov / GG2 / ) in Fhb1 2 In the population, 71 heterozygous individuals with recombination in this segment were screened, and the same marker was used in its F 3 99 homozygous individuals recombined in this segment were obtained through screening.
[0043] (2) Identification of recombinant disease-resistant phenotypes
[0044] The recombinants were inoculated by single flower dripping method when they bloomed. 15 days after inoculation, the number of d...
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