39results about How to "Easy to expand and stable" patented technology

The invention discloses a wheat spikelet number QTL linked SNP molecular marker and application thereof. The SNP molecular marker KASP-1 is located on the short arm of a 2D chromosome of the RefSeqv1.0 genome version, and the 100 bp sequence before and after is shown as SEQ ID NO.46, the polymorphism is C / T and can be obtained by primer amplification shown in SEQ ID NO.1-3. The molecular marker can accurately track the wheat spikelet number QTL QSns.sau-2D.1 to predict the wheat spikelet number and facilitate molecular breeding. The invention also discloses a method for identifying the wheat spikelet number QTL QSns.sau-2D.1 molecular marker. By means of the method, the wheat spikelet number prediction accuracy can be enhanced, so as to quickly screen out wheat varieties or lines with increased spikelet number for breeding, and the breeding process of the wheat high yield varieties can be accelerated.

The invention provides a molecular marker for increasing barley harvest index QTL site under low phosphor condition and an application thereof. The molecular marker is Hvc316, a nucleotide sequence isshown as SEQ ID No:1, the molecular marker is positioned on barley 3H chromosome, and can accurately track the site for increasing a barley harvest index QTL under low phosphor condition, predicts the harvest index characteristic of the barley under the low phosphor condition, and is convenient for molecular design breeding with nutrient efficient utilization. The invention also provides the application of the molecular marker Hvc316 in barley high-yield nutrient efficient breeding. A method provided in the invention is capable of enhancing the accuracy of prediction of the barley harvest index under low phosphor condition, and is convenient for rapidly screening the barley variety or lines used for breeding for increasing the barley harvest index QTL under low phosphor condition, so thata seed selection process of the barley high-yield high-efficiency variety can be greatly accelerated.

The invention discloses a molecular marker SSR52 closely linked with the wheat oligo-tiller gene Ltn3, the nucleotide sequence of which is shown in SEQ ID No.1, and the genetic distance between the molecular marker and the wheat oligo-tiller gene Ltn3 is 0.3cM. Detection and analysis show that the molecular marker can accurately track the wheat oligo-tiller gene, predict the tiller characteristics of wheat, and facilitate molecular design breeding. The invention also discloses a method for identifying the molecular marker of the wheat oligo-tiller gene Ltn3. The method provided by the invention can enhance the accuracy of tiller prediction, improve the success rate of specific plant type breeding, and accelerate the realization of the goal of increasing wheat yield per unit area .

The invention discloses a molecular marker HRM5 closely linked with the wheat oligo-tiller gene Ltn3, the nucleotide sequence of which is shown in SEQ ID No.1, and the genetic distance between the molecular marker and the wheat oligo-tiller gene Ltn3 is 0.35cM. Detection and analysis show that the molecular marker can accurately track the wheat oligo-tiller gene, predict the tiller characteristics of wheat, and facilitate molecular design breeding. The invention also discloses a method for identifying the molecular marker of the wheat oligo-tiller gene Ltn3. The method provided by the invention can enhance the accuracy of tiller prediction, improve the success rate of specific plant type breeding, and accelerate the realization of the goal of increasing wheat yield per unit area .

The invention discloses a KASP molecular marker linked with wheat flag leaf long QTL QFll‑2B and an application thereof. The molecular marker is located on the long arm of chromosome 2B of the RefSeqv1.0 genome. Detection and analysis show that the molecular marker can accurately track the wheat flag leaf length QTL QFll-2B, predict the flag leaf length characteristics of wheat, and facilitate molecular breeding. The invention also discloses a method for identifying the molecular marker of the wheat flag leaf length QTL QFll-2B. The method provided by the invention can enhance the accuracy of the prediction of the flag leaf length, and can more quickly screen out those with longer flag leaves. Wheat varieties or strains are used for breeding, which greatly speeds up the process of wheat breeding.

The invention discloses a SNP molecular marker linked with wheat grain length QTL and its application, belonging to the field of crop molecular genetics and breeding; the SNP molecular marker is KASP-KL-sau1, and the polymorphism is C / T; the KASP-KL ‑sau1 co-localizes with the wheat grain length QTL QKL.sau‑1B on the long arm of the wheat 1B chromosome, and the genetic distance between it and the QTL QKL.sau‑1B is 4 cM, and the nucleotide sequence of 35bp before and after it is shown in SEQ ID NO: 31. The KASP-KL-sau1 disclosed in the present invention is extremely significantly correlated with the grain length QTL QKL.sau-1B, presents the characteristics of closely linked markers, has high accuracy for molecular marker-assisted selection, and can significantly improve the long-grain varieties of wheat. The selection and identification efficiency is high, and the success rate is high. While improving the efficiency of breeding work, it provides a basis for the study of wheat grain length genes.

The invention relates to the technical field of molecular biology, in particular to a molecular marker closely linked to wheat ear extraction QTL QPel.HN.6D and its application. The present invention provides a molecular marker closely linked to wheat ear extraction QTL QPel.HN.6D, the nucleotide sequence of which is shown in SEQ ID NO.1; molecular marker HRM6 is located at the end of wheat ear extraction QTL QPel.HN.6D Within the confidence interval, the two co-localized on wheat chromosome 6D. The molecular marker can accurately track the wheat panicle withdrawal QTL QPel.HN.6D, predict the characteristics of wheat panicle withdrawal, facilitate molecular design and breeding, and enhance the accuracy of ear withdrawal prediction, so as to quickly screen out QTLs with reduced panicle withdrawal Wheat varieties or strains are used for breeding, which can greatly speed up the breeding process of high-yielding wheat varieties with ideal plant types.

The invention discloses a SNP molecular marker KASP-BE-kl-sau2 linked with the main effect QTL of wheat grain length and its application, belonging to the field of crop molecular genetics and breeding. The molecular marker KASP‑BE‑kl‑sau2 polymorphism is C / T, KASP‑BE‑kl‑sau2 co-localizes with wheat grain length QTL Qkl.sau‑BE‑4A on the short arm of wheat chromosome 4A, and is located Within the Qkl.sau‑BE‑4A interval. The molecular marker KASP-BE-kl-sau2 disclosed by the present invention is extremely significantly correlated with the grain length QTLQkl.sau-BE-4A, presents the characteristics of closely linked markers, has high accuracy for molecular marker-assisted selection, and can accurately predict wheat grain length Traits play an important role in improving wheat yield and efficiently screening high-quality wheat varieties with longer grain length, and are also conducive to improving the efficiency of wheat breeding.

The present invention provides a molecular marker closely linked with the wheat grain filling rate QTL QGfr.sicau-6D, the molecular marker is K603, and its nucleotide sequence is shown in SEQ ID NO. 1; the molecular marker K603 is closely related to the wheat grain filling rate The QTLs are tightly linked. Detection and analysis showed that the molecular marker could accurately track the QTL of the grain filling rate of wheat, predict the grain filling rate characteristics of wheat, and then facilitate molecular design breeding. The detection molecular marker K603 can enhance the accuracy of wheat grain filling rate prediction, so that wheat varieties or lines with QTLs that increase grain filling rate can be quickly screened for breeding, which can greatly speed up the breeding process of high-yielding wheat varieties.

The invention discloses a SNP molecular marker related to barley grain β-glucan content and its application. The site of the SNP molecular marker is located at base number 566409962 of chromosome 6 of barley; the SNP base difference is C or T. The present invention discloses for the first time a SNP molecular marker significantly associated with barley grain β-glucan content. The molecular marker is accurate and efficient in detection, convenient and stable in amplification, and can be used for molecular marker-assisted selection to increase barley β-glucan content identification efficiency and accuracy.

The invention discloses a wheat tiller angle QTL QTA‑2B linked molecular marker and its application. The KASP‑sicau1 molecular marker is a SNP molecular marker, and the polymorphism is A / G. It co-localizes with wheat tiller angle QTL QTA‑2B on the long arm of wheat chromosome 2B, and is located in the QTL QTA‑2B interval. The molecular marker KASP-sicau1 disclosed in the present invention is extremely significantly correlated with the tiller angle QTL QTA-2B, presents the characteristics of closely linked markers, has high accuracy for molecular marker-assisted selection, and can significantly improve wheat varieties with larger tiller angles adapted to different environments The selection and identification efficiency is high, and the success rate is high.

The invention provides a molecular marker for increasing barley harvest index QTL site under low phosphor condition and an application thereof. The molecular marker is Hvc316, a nucleotide sequence isshown as SEQ ID No:1, the molecular marker is positioned on barley 3H chromosome, and can accurately track the site for increasing a barley harvest index QTL under low phosphor condition, predicts the harvest index characteristic of the barley under the low phosphor condition, and is convenient for molecular design breeding with nutrient efficient utilization. The invention also provides the application of the molecular marker Hvc316 in barley high-yield nutrient efficient breeding. A method provided in the invention is capable of enhancing the accuracy of prediction of the barley harvest index under low phosphor condition, and is convenient for rapidly screening the barley variety or lines used for breeding for increasing the barley harvest index QTL under low phosphor condition, so thata seed selection process of the barley high-yield high-efficiency variety can be greatly accelerated.

The invention provides a molecular marker closely linked to the wheat grain filling rate QTL QGfr.sicau-7D.1, the molecular marker is KASP705, and its nucleotide sequence is shown in SEQ ID NO. 1; the molecular marker KASP705 is associated with the wheat grain The filling rate QTLs are tightly linked. Detection and analysis showed that the molecular marker could accurately track the QTL of the grain filling rate of wheat, predict the grain filling rate characteristics of wheat, and then facilitate molecular design breeding. The detection molecular marker KASP705 can enhance the accuracy of wheat grain filling rate prediction, so as to quickly screen wheat varieties or lines with QTL increasing grain filling rate for breeding, which can greatly speed up the breeding process of high-yielding wheat varieties.

The invention discloses a wheat stripe rust resistance gene yrZ15-1370 and its molecular marker and application. The wheat stripe rust resistance gene yrZ15‑1370 is located on the long arm of wheat chromosome 6A, and its physical location is 601.47Mb‑603.30Mb in the RefSeqv1.0 genome version. The SNP site of the molecular marker is located in the yrZ15-1370 interval, the polymorphism is A / G, and the molecular marker can accurately track the wheat stripe rust resistance gene yrZ15-1370. The present invention also discloses a primer set for identifying the wheat stripe rust resistance gene yrZ15-1370, using the primer set provided by the present invention can quickly screen out wheat varieties or strains with the wheat stripe rust resistance gene yrZ15-1370 for breeding, It can greatly speed up the breeding process of wheat disease-resistant varieties.

The invention discloses a KASP-Flw-sau6198 molecular marker linked with the main effect QTL of wheat flag leaf width and its application, belonging to the fields of molecular biology and crop genetics and breeding. The molecular marker is a SNP molecular marker, and the polymorphism is A / G, which co-localizes with the wheat flag leaf width QTL QFLW.sau-AM-4B.4 on the long arm of the tetraploid wheat chromosome 4B, and is located in the QTL QFLW .sau‑AM‑4B.4 range. After detection and analysis, it is shown that the molecular marker can accurately track the flag leaf width QTLQFLW.sau‑AM‑4B.4 of wheat, and predict the flag leaf width characteristics of wheat, which is conducive to more rapid screening of wheat varieties or lines with wider flag leaves For breeding, greatly speed up the process of wheat breeding.

The invention discloses a molecular marker linked to QTL for effective tiller number in wheat and its application. The wheat effective tiller number QTL is QTL Qetn-1B.1, the molecular marker is named KASP-1, and its nucleotide sequence is shown in SEQ ID NO.1-3. Detection and analysis showed that the molecular marker could accurately track the wheat effective tiller number QTL Qetn‑1B.1, predict the effective tiller number characteristics of wheat, and facilitate molecular design breeding. The invention also discloses a method for identifying the molecular markers of wheat effective tiller number QTL Qetn-1B.1. The method provided by the invention can enhance the accuracy of prediction of effective tiller number, so as to quickly screen out the QTLs with increased effective tiller number. A large number of wheat varieties or lines are used for breeding, which can greatly speed up the process of breeding high-yield varieties of wheat.