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Molecular marker HRM7 of barley grain length gene LkI2 and application of molecular marker

A molecular marker, barley kernel technology, applied in the application, determination/inspection of microorganisms, angiosperms/flowering plants, etc., to achieve the effect of improving the efficiency of selection and identification, high success rate and high accuracy

Inactive Publication Date: 2016-04-27
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the reported grain length-related gene QTL is located on 7 chromosomes, but there is no report to do more in-depth research on the related loci to complete the fine mapping work

Method used

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  • Molecular marker HRM7 of barley grain length gene LkI2 and application of molecular marker
  • Molecular marker HRM7 of barley grain length gene LkI2 and application of molecular marker
  • Molecular marker HRM7 of barley grain length gene LkI2 and application of molecular marker

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Experimental program
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Effect test

Embodiment 1

[0049] This embodiment is used to illustrate the obtaining method of barley grain length gene Lk12 and molecular marker HRM7:

[0050] (1) Using long-grain long wild barley AWCS276 as the female parent and barley Baudin as the male parent to cross hybrids to obtain hybrid F1, F1 generation F2 was obtained by selfing a single plant, and the F8 generation RIL containing 130 lines was obtained by single-seed propagation method Populations constitute genetic mapping populations.

[0051] (2) Extract the DNA of each strain of the genetic mapping population with the CTAB method, use the DArT chip technology, and use the DNA of the parents AWCS276 and Baudin as a template to perform genotyping to obtain the genotype data of the RIL population . The band pattern of AWCS276 is marked as A, and the band pattern of parent Baudin is marked as B. The strain band type of F8 population derived from AWCS276 was marked as A, the strain derived from Baudin was marked as B, and the heterozygou...

Embodiment 2

[0061] 1.1 Extraction of DNA

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Abstract

The invention relates to the field of barley molecular breeding, and in particular discloses a molecular marker HRM7 of barley grain length gene LkI2 and application of the molecular marker. The nucleotide sequence of the molecular marker HRM7, which is closely linked with the barley grain length gene LkI2, is as shown in SEQ ID No.1. Upon detection and analysis, the molecular marker can accurately track the barley grain length gene and predict the grain length traits of the barley, so as to facilitate molecular designing and breeding. The invention also discloses a method for identifying the molecular marker of the barley grain length gene LkI2; by virtue of the method provided by the invention, the accuracy of grain length prediction is enhanced, so that barley varieties or lines having enhanced grain length QTL can be conveniently and rapidly screened out; and the process of selecting high-yield barley varieties can be greatly accelerated.

Description

technical field [0001] The invention relates to the field of barley molecular breeding, in particular to a molecular marker HRM7 of barley grain length gene Lkl2 and its application. Background technique [0002] Barley is one of the oldest food crops in the world and has multiple uses: it is a combination of food, feed, beer raw materials, medicine and health food. In terms of its yield, barley is the fifth largest crop in the world; in terms of its planting area, it is the fourth largest cereal crop planted and produced in the world 2 , with a total annual output of about 150 million tons, second only to corn, rice, and wheat. For nearly half a century, human production and demand for barley showed a continuous growth trend. Statistics in 2008 show that the total annual demand for barley is 27.4 million tons, which is second only to corn in cereal crops. Therefore, the selection of high-yielding barley varieties has always been the focus of breeders. At the same time, a...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12N15/82A01H5/00
CPCC12Q1/6851C12Q1/6895C12Q2600/13C12Q2600/158C12Q2531/113C12Q2545/113C12Q2563/107
Inventor 刘亚西周红林宇王智强武方琨卢艳丽陈光登马建江千涛魏育明郑有良
Owner SICHUAN AGRI UNIV
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