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KASP molecular marker linked with wheat stripe rust resistant QTL and application

A molecular marker and wheat technology, which is applied in the field of crop molecular genetic breeding, can solve the problems of limiting the use of resistance sites and the efficient selection of disease-resistant sites, and achieves improved selection and identification efficiency, convenient and stable amplification, and accuracy high effect

Active Publication Date: 2022-01-07
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the QYr.sicau-2AS tightly linked KASP molecular marker, and it is impossible to realize the rapid and efficient selection of this resistance locus, which greatly limits the use of this resistance locus in wheat disease resistance breeding. use

Method used

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  • KASP molecular marker linked with wheat stripe rust resistant QTL and application
  • KASP molecular marker linked with wheat stripe rust resistant QTL and application
  • KASP molecular marker linked with wheat stripe rust resistant QTL and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Acquisition of QYr.sicau-2AS-linked KASP molecular marker for wheat stripe rust resistance locus

[0041] 1. Test materials

[0042] The hybrid F 1 , F 1 F 2 , using single grain transfer to F 6 Generations, recombinant inbred lines containing 154 individual plants were obtained to form a genetic mapping population.

[0043] 2. Identification of stripe rust resistance phenotypes at seedling stage and adult plant stage

[0044] Identification of resistance at seedling stage: at the 2nd leaf stage of seedling stage, the physiological race CYR34 of stripe rust (from Gansu Academy of Agricultural Sciences Plant Protection Institute), which is currently popular in my country, was used to spray on Shumai 126, Taichang 29 and 154 recombinant self Cross lines were inoculated with stripe rust. When the susceptible line SY95-71 (selected by Sichuan Agricultural University) is completely diseased (14-18 days after inoculation), the resistance to stripe rust will be...

Embodiment 2

[0076] Example 2: Application of Wheat Stripe Rust Resistance Locus QYr.sicau-2AS Linked KASP Molecular Marker

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Abstract

The invention belongs to the technical field of crop molecular genetic breeding, and discloses a KASP molecular marker linked with wheat stripe rust resistance QTL and application. The nucleotide sequence of the KASP molecular marker comprises a first nucleotide sequence and a second nucleotide sequence; and the first nucleotide sequence is SEQ ID NO: 1, and the second nucleotide sequence is SEQ ID NO: 2. The molecular marker linked with wheat stripe rust resistance QTL and located on a wheat 2A chromosome is a flanking marker of a stripe rust resistance site QYr.sicau-2AS on a short arm of the wheat 2A chromosome, and the linkage degree is high. The marker can be used for detecting the stripe rust resistance site QYr.sicau-2AS on the wheat 2A chromosome, plants with the loci are rapidly screened, and then the wheat disease resistance breeding efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of crop molecular genetic breeding, and in particular relates to a KASP molecular marker linked with wheat stripe rust resistance QTL and its application. Background technique [0002] At present, wheat stripe rust is an airborne fungal disease caused by Puccinia striiformis f.sp.tritici (Pst), which seriously endangers wheat production. Discovering, identifying and utilizing effective disease resistance genes, developing molecular markers closely linked to them and applying them to breeding wheat varieties with long-lasting resistance to stripe rust are the most effective measures to control the damage of stripe rust. [0003] So far, 83 stripe rust resistance genes (Yr1-Yr83) have been officially named. Due to the rapid pathogenic variation of stripe rust and the emergence and prevalence of new virulent races, most of the stripe rust resistance genes have lost their resistance and lost their value in dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107Y02A40/10
Inventor 伍碧华王宇凡黄林胡燕灵龚方仪贺靖舒
Owner SICHUAN AGRI UNIV
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