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SNP molecular marker and application thereof

A molecular marker and barley technology, applied in the fields of molecular biology and genetic breeding, can solve the problems of quality deterioration, mineral nutrition metabolism disorder, crop yield reduction, etc., and achieve the effects of convenient and stable amplification, accurate and efficient detection, and improved identification efficiency.

Pending Publication Date: 2021-10-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The damage caused by salt stress to crops is mainly osmotic stress and ion toxicity, as well as the resulting secondary oxidative stress, which in turn causes photosynthetic physiological damage, mineral nutrient metabolism disorders, etc., and ultimately leads to reduced crop yield and quality deterioration

Method used

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  • SNP molecular marker and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Test materials

[0035] 100 accessions of global barley micro-core germplasm resources were used as materials, including 34 accessions of two-row barley and 66 accessions of six-row barley.

[0036] (2) Determination of traits

[0037] with 2%H 2 o 2 The solution sterilizes barley seeds and germinates them on wet filter paper in a growth chamber. Seven days after the seeds germinated, the seedlings were transplanted into 15 L black plastic containers containing one fifth of Hoagland's solution (pH 6.0), and the hydroponic nutrient solution was changed every 3 days. In the autumn of 2019, in the net room and greenhouse (250μmol·m -2 the s -1 fluorescent lamp, 23°C 14h / 18°C 10h) for water seedling cultivation and salt treatment test.

[0038] Add 100 mM sodium chloride to the hydroponic solution when the seedlings grow for 14 days, add sodium chloride to 200 mM the next day and continue the treatment for two weeks. Rinse the plants with deionized water, take th...

Embodiment 2

[0044] (1) Test materials

[0045] Using 14 global barley micro-core germplasm resources as materials, the relative dry matter weight and sodium content of roots under salt stress were measured and the target area of ​​S1H_527797394 was analyzed. Specifically, as shown in Table 1, it includes 7 parts of high relative dry matter weight and sodium content materials and 7 parts of low relative dry matter weight and sodium content materials.

[0046] Table 1 14 barley germplasm materials with different salt tolerance

[0047] barley germplasm SNP detection results Na + content

relative dry weight Indicator type W09 T 0.37 52.47 high W10 T 0.67 52.29 high W30 T 0.34 52.00 high W32 T 0.46 58.33 high W33 T 0.47 50.77 high W41 T 0.52 49.22 high W58 T 1.22 46.29 high W81 C 0.18 38.90 Low W15 C 0.18 43.05 Low W50 C 0.19 36.11 Low W97 C 0.22 42.00 Low W55 C 0.25 ...

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Abstract

The invention discloses an SNP molecular marker and application thereof. The locus of the SNP molecular marker is located at the 527797394th basic group of a barley chromosome I; the SNP base difference is C or T. According to the invention, an SNP locus with an improvement effect on salt tolerance is identified through whole-genome association analysis of relative dry matter weight and sodium content of roots under barley salt stress. The invention discloses an SNP molecular marker which is obviously associated with the relative dry matter weight and the sodium ion content of the root under the stress of barley salt, and the molecular marker is accurate and efficient in detection and convenient and stable in amplification. The SNP molecular marker can be used for molecular marker-assisted selection, and the identification efficiency of different salt-tolerant barley varieties is improved.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and genetic breeding, in particular to a SNP molecular marker and its application. Background technique [0002] Soil salinization is one of the main adversity stresses limiting global crop production. It is of great significance to study the salt-tolerant mechanism of crops and cultivate new salt-tolerant varieties of crops for world food security. To develop and utilize salt-tolerant genetic resources and breed new varieties of salt-tolerant crops, the salt-tolerant mechanism of crops must be clarified. The damage caused by salt stress to crops is mainly osmotic stress and ion toxicity, as well as the resulting secondary oxidative stress, which in turn causes photosynthetic physiological damage, mineral nutrient metabolism disorders, etc., and ultimately leads to reduced crop yield and quality deterioration. [0003] As an ancient and one of the most salt-tolerant crops, barley has t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2600/172C12Q2531/113C12Q2535/122Y02A40/90
Inventor 沈秋芳屠一珊傅良波张国平
Owner ZHEJIANG UNIV
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