125 results about "Genetics genomics" patented technology

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY1. The lilium regale WRKY transcription factor gene LrWRKY1 has a nucleotide sequence shown as SEQ ID NO: 1, and codes a protein with a nucleotide sequence shown as SEQ ID NO: 2. According to the lilium regale Wilson WRKY transcription factor gene LrWRKY1, functional genomics related technical research verifies that the LrWRKY1 gene has the function of increasing the fungus resistance of plants; when the antifungal LrWRKY1 gene is constructed to a plant expression vector and is transferred to tobaccos for overexpression, transgenic tobacco plants have strong in-vitro antifungal activities, and experimental results show that the LrWRKY1 overexpressed transgenic tobaccos have obvious inhibiting effects on the growth of four fungi including botryosphaeria, sclerotinite, botrytis cinerea and fusarium oxysporum.

Expressed Sequence Tags (ESTs) isolated from soybean are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

Expressed Sequence Tags (ESTs) isolated from maize are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

The invention discloses application of panax japonicus transcription factor gene PjERF1, namely, application in improving the expression quantity of key enzyme genes in the biological synthesis of panax japonicus saponins, and increasing the content of saponins in panax japonicus callus. The nucleotide sequence of the PjERF1 gene is shown as SEQ ID NO: 1, and AP2 / ERF transcription factors are coded. Through the adoption of functional genomics and techniques related to metabolic engineering, a panax japonicus PjERF1 transcription factor is proved to have the effect of positively regulating the biological synthesis of panax japonicus saponins; when the panax japonicus PjERF1 transcription factor gene disclosed by the invention is established on a plant expression vector and transferred into the panax japonicus callus for over expression, the expression quantity of the key enzyme genes in the synthetic route of panax japonicus saponins is increased, and the yield of panax japonicus saponins is increased.

The invention discloses a lilium regale gene Lr14-3-3 with antifungal activity. The gene Lr14-3-3 has the nucleotide sequence as shown in SEQ ID NO:1 and encodes protein 14-3-3. A relevant technology of functional genomics proves that the gene Lr14-3-3 has a function of improving the plant antifungal activity. The antifungal gene Lr14-3-3 is constructed to a plant expression vector and is transferred into tobacco to perform overexpression; the transgenic tobacco plant has strong in vitro antifungal activity; and the transgenic tobacco expressing the Lr14-3-3 has obvious inhibition effects on the growth of botryosphaeria dothidea, phomopsis fungi, fusarium oxysporum and alternaria.

The invention relates to rapid identification of anti-powdery-mildew gene of Brachypodium distachyon, relates to the knowledge of plant comparative genomics, genetics, biological information and other subjects, and belongs to the scientific field of plant biotechnology. The rapid identification of anti-powdery-mildew gene of Brachypodium distachyon comprises the main steps of: (1) downloading full genome sequences of Brachypodium distachyon and collecting MLO (mildew resistance locus o) type gene; (2) identifying the MLO type gene; (3) analyzing the MLO type gene historical development relation; and (4) comparing the MLO type powdery mildew gene. According to the rapid identification of anti-powdery-mildew gene of Brachypodium distachyon provided by the invention, the excavation period of the anti-powdery-mildew gene of Brachypodium distachyon is effectively shortened, and the rapid identification of the powdery mildew gene is facilitated; the identified powdery mildew gene can be used for developing corresponding coseparation functional markers (SNP (single nucleotide polymorphism), SCAR (sequence-characterized amplified region), and the like), and can also be rapidly used for assisted selection of molecule markers of anti-powdery-mildew gene, and the accuracy is high; development of multi-resistance breeding materials can be carried out by combining with other anti-disease gene molecule markers, the breeding time is shortened and the breeding efficiency is improved; and foundation is established for expounding the molecular mechanism of the anti-powdery-mildew gene of Brachypodium distachyon.

The invention discloses a method for constructing a pepper mutant library from ethylmethane sulfonate. The method is characterized in that a Zunla 1 is used as a mutagenesis object; on the premise ofpointing out the influence of treating fluid dose and excluding space of each seed on germination percentage, semi-lethal dose is determined by comparing the germination percentage of pepper seeds with EMS treating fluid with different concentrations at different mutagenesis time; the pepper seeds are treated by mutagenesis of the semi-lethal dose; mutation frequency and mutation types of M2 generation are investigated, a mutant capable of stably inheriting of M4 generation is identified and a mutant library is constructed; the mutation types of leaves, stems, fruits, growth period, flower organs, fertility and the like are obtained and create abundant materials for functional genomics reach of pepper; and meanwhile, partial beneficial mutation can be directly applied to breeding practice.

The invention provides a method for obtaining a vascular cambium of a woody plant and application thereof. According to the method, the vascular cambium in a woody plant tissue paraffin section containing the vascular cambium is obtained through a laser microdissection method. According to the method, the pure cambium tissue of the woody plant neolamarckia cadamba is successfully separated throughorganic combination of paraffin section and laser microdissection technologies. According to the method, the paraffin section process is optimized, LMD parameters are debugged, a laser microdissection technical system of the vascular tissue cells of the seedlings of the neolamarckia cadamba is established, and a high-quality vascular cambium is obtained. The application of the method disclosed bythe invention in similar tissues of woody plants is not reported, the tissue and organ cells obtained by the method are relatively high in homogeneity and complete in cell structure, and a foundationis laid for further researching molecular biology and genomics research of cambium.