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Method for quickly identifying manihot esculenta mildew-resistance locus (MLO) gene by applying comparative genomics

A powdery mildew resistance gene and cassava technology, applied in genetic engineering, plant gene improvement, application, etc., can solve the problems of difficult cloning and long time, and achieve the effect of shortening the breeding period, shortening the time, and improving the efficiency of identification

Inactive Publication Date: 2012-11-28
常熟市支塘镇新盛技术咨询服务有限公司
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Problems solved by technology

However, because the basic research on cassava is not deep enough, it is not only time-consuming to use these methods but also difficult to accurately clone these genes

Method used

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  • Method for quickly identifying manihot esculenta mildew-resistance locus (MLO) gene by applying comparative genomics
  • Method for quickly identifying manihot esculenta mildew-resistance locus (MLO) gene by applying comparative genomics
  • Method for quickly identifying manihot esculenta mildew-resistance locus (MLO) gene by applying comparative genomics

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Embodiment Construction

[0031] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify cassava powdery mildew genes. The specific implementation process is as follows:

[0032] 1) Collection and identification of cassava MLO-type genes

[0033] In order to obtain all the members of the MLO gene family of cassava, we first constructed an HMM model using the MLO gene sequences of Arabidopsis thaliana and the MLO gene sequences of tomato, pea, rose, pepper, and lotus root for resistance to powdery mildew. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC4, 93 TC315912, TC322759, TC322059, TC330654, TC282713, TC293173, TC281861, TC283253, TC283383, TC28503...

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Abstract

The invention discloses a method for quickly identifying a manihot esculenta mildew-resistance locus (MLO) gene, relates to knowledge of plant comparative genomics, genetics, bioinformatics and the like and belongs to the field of plant biotechnology science. The method mainly comprises the following steps of: 1) downloading a manihot esculenta whole genome sequence, and collecting the MLO gene; 2) identifying the MLO gene; 3) identifying the MLO gene according to the MLO gene phylogenetic relationship; and 4) comparing the MLO genes. By the method, the discovery cycle of the manihot esculenta MLO gene is shortened effectively, and the MLO gene can be quickly identified; corresponding coseparation functional markers (single nucleotide polymorphism (SNP) and specific combining ability (SCA)) can be developed by the identified candidate MLO gene, and the method can be quickly used for molecular marker auxiliary selection of the MLO gene, and is high in accuracy; by combining other disease-resistant gene molecular markers, multiresistance breeding materials can be prepared, the breeding cycle is shortened, and the breeding efficiency is improved; and the foundation is laid for elaborating a manihot esculenta MLO gene molecular mechanism.

Description

technical field [0001] The present invention uses methods such as plant comparative genomics, genetics, bioinformatics, and candidate gene strategies to quickly identify cassava powdery mildew genes by means of sequencing the whole genome sequence of cassava, and mainly involves the download of the whole genome sequence of cassava and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Cassava is a shrubby perennial plant of the genus Cassava in the Euphorbiaceae family. It is native to tropical America and is widely cultivated in tropical regions all over the world. Its edible roots are used to grind cassava flour, make bread, provide cassava starch and starch for washing, and even alcoholic beverages. There are about 160 species of cassava, all of which are sun-loving plants native to tropical America. Ceara ...

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12Q1/68
Inventor 钱孝英
Owner 常熟市支塘镇新盛技术咨询服务有限公司
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