Method for constructing pepper mutant library from ethylmethane sulfonate
A technology of ethyl methanesulfonate and mutants, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problem that there is no mutant library of Chaotian pepper, the quality of fruit products is reduced, and research on pepper breeding is less, etc. problems, to achieve the effect of being conducive to reasonable dense planting, hindering the feeding and reproduction of aphids, and reducing the occurrence of virus diseases
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[0052] (1) Preparation of EMS treatment solution: Mix 305ml of disodium hydrogen phosphate solution with a concentration of 0.2mol / L and 195ml of sodium dihydrogen phosphate solution with a concentration of 0.2mol / L, add 0, 6, 7, 8, 9, 10, 11 and 12ml of EMS, dilute to 1 liter with distilled water, the volume ratio concentration is 0, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, pH EMS mutagenesis treatment solution of 7.0.
[0053] (2) EMS mutagenesis treatment:
[0054] A. Select the plump "Zun La No. 1" Chaotian pepper dried seeds, 50 grains each as 1 portion, put them into 15*10cm nylon mesh bags, put them in warm water at 55°C and stir continuously, and soak the seeds for 15 minutes;
[0055] B. dry the surface moisture of each seed after soaking in warm soup with filter paper;
[0056] C. in the 50ml round-bottom centrifuge tube, inject the EMS mutagenesis treatment solution that the volume ratio concentration is 0%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, and pH is 7.0...
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