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Method for constructing pepper mutant library from ethylmethane sulfonate

A technology of ethyl methanesulfonate and mutants, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problem that there is no mutant library of Chaotian pepper, the quality of fruit products is reduced, and research on pepper breeding is less, etc. problems, to achieve the effect of being conducive to reasonable dense planting, hindering the feeding and reproduction of aphids, and reducing the occurrence of virus diseases

Pending Publication Date: 2018-10-09
GUIZHOU SERICULTURE RES INST GUIZHOU PEPPER RES INST
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AI Technical Summary

Problems solved by technology

Although Chaotian pepper has outstanding flavor quality and industrial advantages, there are still some deficiencies in production practice: first, the wrinkled fruit surface reduces the commercial quality of the fruit; Third, the loose plant type is not conducive to reasonable dense planting, which affects the high yield of pepper
However, ethyl methanesulfonate has little research on capsicum breeding, and there is no relevant report on the mutant library of Chaotian pepper

Method used

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  • Method for constructing pepper mutant library from ethylmethane sulfonate
  • Method for constructing pepper mutant library from ethylmethane sulfonate
  • Method for constructing pepper mutant library from ethylmethane sulfonate

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Embodiment 1

[0052] (1) Preparation of EMS treatment solution: Mix 305ml of disodium hydrogen phosphate solution with a concentration of 0.2mol / L and 195ml of sodium dihydrogen phosphate solution with a concentration of 0.2mol / L, add 0, 6, 7, 8, 9, 10, 11 and 12ml of EMS, dilute to 1 liter with distilled water, the volume ratio concentration is 0, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, pH EMS mutagenesis treatment solution of 7.0.

[0053] (2) EMS mutagenesis treatment:

[0054] A. Select the plump "Zun La No. 1" Chaotian pepper dried seeds, 50 grains each as 1 portion, put them into 15*10cm nylon mesh bags, put them in warm water at 55°C and stir continuously, and soak the seeds for 15 minutes;

[0055] B. dry the surface moisture of each seed after soaking in warm soup with filter paper;

[0056] C. in the 50ml round-bottom centrifuge tube, inject the EMS mutagenesis treatment solution that the volume ratio concentration is 0%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, and pH is 7.0...

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Abstract

The invention discloses a method for constructing a pepper mutant library from ethylmethane sulfonate. The method is characterized in that a Zunla 1 is used as a mutagenesis object; on the premise ofpointing out the influence of treating fluid dose and excluding space of each seed on germination percentage, semi-lethal dose is determined by comparing the germination percentage of pepper seeds with EMS treating fluid with different concentrations at different mutagenesis time; the pepper seeds are treated by mutagenesis of the semi-lethal dose; mutation frequency and mutation types of M2 generation are investigated, a mutant capable of stably inheriting of M4 generation is identified and a mutant library is constructed; the mutation types of leaves, stems, fruits, growth period, flower organs, fertility and the like are obtained and create abundant materials for functional genomics reach of pepper; and meanwhile, partial beneficial mutation can be directly applied to breeding practice.

Description

technical field [0001] The invention belongs to the technical field of crop mutation breeding, and relates to a method for constructing a capsicum mutant library by using ethyl methanesulfonate. Background technique [0002] Pepper is one of the most important vegetable crops in my country, and its planting area accounts for about 10% of the total vegetable planting area, which plays an important role in ensuring the annual balanced supply of vegetables in our country. Guizhou has the largest planting area of ​​pepper in the country, and the planting area of ​​Chaotian pepper accounts for about 40% of the total pepper planting area in the province. Therefore, Chaotian pepper occupies a pivotal position in the pepper industry in Guizhou Province and even the whole country. What's more worth mentioning is that Guizhou chaotian pepper has a strong aroma and moderate spiciness, and its unique flavor quality is irreplaceable for Guizhou people. Although Chaotian pepper has outst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 黄冬福付文婷何建文梁郸娜范高领詹永发涂祥敏杨万荣周光萍
Owner GUIZHOU SERICULTURE RES INST GUIZHOU PEPPER RES INST
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