109results about How to "Good plant type" patented technology

The present invention discloses breeding process of rice blast-resisting high quality and high yield round-grained rice variety Zhendao-10. The breeding process breeds rice variety featuring high resistance to rice blast, high quality and high yield. The breeding process includes the following steps: 1. hybridizing Zhendao-99 hybrid rice variety with early maturing, high yield and good taste as the female parent and Zhen-540 hybrid rice variety with excellent appearance quality as the male parent to obtain F1; and 2. selfing with the F1 for six generations to obtain the rice blast-resisting high quality and high yield round-grained rice variety Zhendao-10.

The invention discloses a breeding method of high-quality double-low disease-resistant high-yield lodging-resistant rape cultivar 'Zhen 2405'. The bred high-quality double-low rape cultivar has the advantages of full and round seed grain, heavy thousand grain weight, black seed grain, good marketability, excellent disease resistance, stable yield, good lodging resistance, good plant-type trait, good maturity performance, consistent maturity and suitability for the mechanized harvest. The method comprises the following steps: A) selecting the early-mature high-yield rapeseed line 'Zhen 9701' as the maternal plant, which has high resistance to Sclerotinia sclerotiorum and lodging and has compact plant-type trait, selecting rape line F1 as the male parent, which has good agronomic characters and high-yield trait, and cross-breeding to obtain a rape line F0; and B) sowing the rape line F0, sowing the excellent individual plants after the two-row sowing of the rape line F0, and self-inbreeding for 6 generations to obtain the high-quality double-low high-yield rape cultivar (Brassica napus) 'Zhen 2405' with excellent resistance to Sclerotinia sclerotiorum and lodging.

A method for the reproduction of wild bigleaf tea tree with high yield and quality includes such steps as cutting the wooden twig of wild male-parent bigleaf tea tree to become segments with a leaf to obtain cuttage twigs, rooting, inserting them in nursery bed, arranging sunshade net above the nursery bed, moistening the nursery bed, conventional managing, and transplanting.

The invention discloses a greenhouse psammophyte culture medium. Four different matrix layers, namely a base layer, an original soil layer, a nutritive layer and a covering layer, are sequentially paved from bottom to top, wherein the base layer is one or a mixture of more than one of quartz, feldspar, mica, kaolinite, limestone and granite, the thickness of the base layer is 30-80cm, and particle size is 1-5cm; the original soil layer is obtained by mixing chestnut soil, gray desert soil and desert sandy soil in volume ratio of (1-2):10:(1-3), and the thickness of the original soil layer is 20-60cm; the nutritive layer is a mixture of organic matters, inorganic minerals, inorganic fertilizer, microelement fertilizer and microbial fertilizer, and the thickness of the nutritive layer is 20-100cm; and the covering layer is formed by vermiculite and / or volcanic rock, and the thickness of the covering layer is 5-10cm. The greenhouse psammophyte culture medium is easy to produce and low in cost and can obviously improve the transplanting survival rate of greenhouse landscape psammophytes when applied to cultivation of the greenhouse landscape psammophytes.

The invention relates to the field of plant genetic engineering and specifically relates to an application of an osa-MIR167a gene for regulating and controlling plant type of paddy rice. The osa-MIR167a gene has several homologous genes in paddy rice genome and belongs to a same gene family MIR167 as an arabidopsis thaliana ath-MIR167 gene. The osa-MIR167a gene has a function of regulating and controlling the plant type of paddy rice and the nucleotide sequence thereof is shown in the sequence table SEQ ID NO:1 while the core sequence formed by transcription and splicing is shown in the sequence table SEQ ID NO:3. An ideal plant type and increased yield are achieved by changing the expression models of the osa-MIR167a gene or other members in the osa-MIR167a gene family through gene engineering and regulating and controlling the tillering angle, the height and the effective tillering number of the plant. The invention discloses the application of the osa-MIR167a gene for regulating and controlling the plant type of paddy rice.

The invention discloses plant development associated protein, an encoding gene and application thereof. The protein provided by the invention is the protein of (a) or (b): (a) the protein formed by amino acid sequences shown as a third sequence in a sequence table; or (b) plant development associated derived protein formed through the substitution and / or lack and / or addition of one or a plurality of amino acid residue radicals on the protein of the third sequence in the sequence table. The plant development is embodied by the properties of the strain height of developed strains and / or the size of leaf angles and / or the fertility of the developed strains and / or the size of specific organs. The invention also obtains a reccanbinant vector containing the encoding gene OsIBP1, and when the reccanbinant vector is used for converting target plants, transgenic plants with shortened strain height of the developed strains and / or reduced leaf angles and / or reduced fertility of the developed strains and / or reduced size of the specific organs can be obtained. Thereby, OsIBP1 can be used as a potential molecular breeding tool for improving the plant strain types and improving the plant yield.

The invention discloses a method for breeding and seed production of superior multi-resistance high-yield two-line hybrid rice. According to the method, superior rice-blast-resistant high combining ability photo-thermo sensitive genic male sterile line-guangzhan 63S-4 is taken as a female parent to hybridize with a superior bacterial blight-resistant strong and excellent restorer line-zhenhui 832, thus obtaining the two-line hybrid rice-liangyou 832 with high quality, high rice blast and bacterial blight resistance and high yield. The rice cultivated by the method has the advantages of good comprehensive yielding ability, moderate maturation period, high quality, good commodification and strong stress resistance.

The invention discloses a method for breeding high-yield and disease-resistant glutinous rice, and belongs to a crop breeding method. The method disclosed by the invention comprises the following steps: selecting disease-resistant and high-yield japonica rice as a female parent, selecting blast-resistant and high-yield round-grain glutinous rice as a male parent, and hybridizing the japonica rice with the glutinous rice to obtain a generation F1; carrying out manual screening, rice stripe disease identification and bacterial leaf blight artificial inoculation identification, and selfing in a generation F5; and further screening by identifying bacterial leaf blight, rice stripe disease and rice blast, and identifying quality and taste so as to obtain a target variety. By adopting the breeding method disclosed by the invention, the bred glutinous rice has the advantages of excellent quality, good taste and good commodification; the selected waxy material has the advantages of good plant type, good ripe phase, strong disease resistance, strong yielding ability, good yield stability, coordinated grain structure and strong auto-regulation ability, and ripe stage is combined with taste quality during yield identification; and thousand seed weight of the bred material is more than 26g after screening for years.

The invention discloses breeding and seed production methods of quality multi-resistant high-yield hybrid rice indica Zhen you 184. The rice bred through the method has good comprehensive yielding ability, moderate mature period, fine quality, good commercial value and strong stress resistance. The breeding method includes the steps of taking quality rice-blast-resistant sterile line Zhenxian 1A with high combining ability as the female parent to hybridize with quality bacterial-blight-resistant high-heterosis restorer line Zhen hui 084 to obtain hybrid rice indica Zhen you 184 with fine quality, strong rice blast resistance and bacterial blight resistance and high yield. The seed production method is as follows: seeding the male parent Zhen hui 084 in two periods, seeding the male parent in the I period at the end of April and then seeding the male parent in the II period 8-12 days later. The female parent is seeded 14-18 days after the male parent in the I period, the leaf age difference is about 3.0-3.5, the ratio of the rows of the male parent and the female parent in the seed field is 2:12-14, the male parent of the I period and the II period are in alternate single-plant transplantation, the row pitch of the female parent plants is 12-15cmX 16-19cm, and fewer-seedling transplanting is adopted. The seeds are isolated strictly, care is paid to remove the impurities, appropriate amount of '920' is sprayed through artificial supplementary pollination measure, and disease and insect control work is paid attention to.

The invention provides a yield-increasing regulator used for expanding the ear of grains, increasing the grains and resisting the lodging for the corn, and a preparation method and an application thereof. The regulator contains the following components by certain weight percentage: a plant growth regulator, a promoting agent, a speader, absolute ethyl alcohol and water for the rest; wherein, the growth regulator is florel, gibberellin and 6-furfuryl aminopurine, the promoting agent and the speader are selected from tween 20, tween 40, tween 60, tween 80 or tween 85. The regulator has the double functions of strengthening the lodging-resistant and increasing yield, and is characterized by innocuity, no side effect, low cost, convenient usage and the like.

The present invention provides a method for artificially developing novel brassica napus. The excellent characters of cabbage and mustard type rape are integrated for obtaining novel brassica napus. The hybridization compatibility when the mustard type rape and cabbage are used for executing interspecific hybridization is higher than the hybridization compatibility when the Chinese cabbage and cabbage are used for executing interspecific hybridization. The interspecific hybrid is easily obtained, and simultaneously, the advantages of drought resistance, cold resistance, excellent strain type, etc. of the mustard type rape can be introduced into the brassica napus. The method of the invention also has the advantages of stable heredity, high speed, large area of character variation and facilitation for selecting the brassica napus with excellent charcter. Furthermore, the chromosomes of AC chromosome set of brassica napus obtained from the method of the invention respectively come from the mustard type rape and cabbage, have larger difference from the prior brassica napus. The use of hybrid vigor of genome of brassica napus is facilitated and the production level of cabbage is increased.

The invention relates to a living body biological organic fertilizer, comprising tetramycin live thallus generated by carrying out spread cultivation on Streptomyces ahygroscopicus subsp. Wuzhouensis n. subsp (common name: tetramycin thallus), tetramycin thallus protein, moisture, and the balance of poultry excrement and straw decomposed materials, wherein the tetramycin live thallus is no less than 0.02billion / g, the tetramycin thallus protein is no less than 10%, the moisture is no more than 30%, the sum of basic components is 100%, coliform group number of the poultry excrement is no more than 100 / g, and the content of organic matters is no less than 25.0%. Thanks to growth and reproduction of functional microorganism, dominant biotic population forms in a micro ecosystem of crop plant rhizosphere soil, so as to inhibit growth and reproduction of other harmful microorganisms, and even generating antagonism towards partial harmful pathogenic bacteria to reduce harm possibility of the harmful microorganisms. Various high-tension products secreted in the micro ecosystem of the crop plant rhizosphere soil in the process of growth and reproduction of the functional microorganisms in fertilizers can stimulate growth of crop plants and improve bad environment resistance capability of the crop plants. The crop plants which are applied with the product of the invention grow robustly, and have good plant type, reasonable plant group and stronger disease resistance.

The invention discloses a breeding method of the japonica rice variety Zhendao No.12 characterized in good quality, good taste, rice blast resistance and high production. The rice bred by the method has the advantages of good quality, good taste, high commodity value; excellent disease resistance, good yield stability and fertility, coordinated grain structure, auto-regulation; good plant type and maturation appearance. The breeding method of the invention includes the following steps: (A) selecting the self-selected Zhenxiang 24 of the late-maturing middle japonica rice characterized in early mature period, high production, rice blast resistance and good taste as the female parent, crossbreeding with the strain Zhen 511 characterized in rice blast resistance, good quality and appearance and good taste and obtaining 60 grains of F0 seeds; (B) fry soaking, sprouting and seed sowing of the F0 seeds, multiple crossing with the Wuyujing No. 8 variety with high production but bad quality, self-breeding for eight generations and obtaining the japonica rice characterized in extra quality, good taste, rice blast resistance and high production, that is the Zhendao No.12.

The invention discloses a three-dimensional planting method for olea europaea. The three-dimensional planting method includes the following steps of suitable sloping field selecting, wherein a plurality of bench terraces distributed in a step shape are prepared on the surface of a sloping field, and surface soil of the bench terraces is deeply turned; planting pit digging, wherein planting pits for planting olea europaea are dug in the surfaces of the prepared bench terraces, a row of planting pits are dug in each step, and water draining devices are embedded at the bottoms of the planting pits; backfilling, wherein the planting pits are filled with soil for planting olea europaea, and soil is 10 cm to 20 cm higher than the ground of the bench terraces; planting; managing; picking. By means of the three-dimensional planting method, the reasonable planting density can be achieved in olea europaea orchards, the ventilation and light transmittance in orchards can be improved accordingly, and ideal plant types are kept; irrigation, water draining and concentrated fertilization are facilitated, growth of root systems of olea europaea is facilitated, olea europaea plants are robust, the planting density is increased by 27.2% compared with the planting density of common orchards, and the yield is increased by 25% or above.

An application of the homogenetic tetrapolid hybrid vigor of paddy rice in preparing seeds includes such steps ad artificial reduplication of excellent diploid rice to obtain homogenetic tetraploid, improving by several generations, selectively culturing the trilinear homogenetic tetraploid male sterility line, further improving, culturing the homogenetic tetraploid hybrid combination and the newvariety with ultra-strong dominance, sterile reproduction between homogenetic tetraploid metal sterility line and maintenance line, and preparing the homogenetic tetraploid hybrid rice seeds with homogenetic tetraplid male sterility line and recovery line. The resultant seed features, ultra-strong hybrid vigor, high resistance and adaptability, and high protential to increase yield.

The invention discloses a method for breeding and producing of hybrid seeds for hybrid rice II super 42. The breeding method comprises the following steps: A. taking Zhenhui129 as female parent and Zhenhui084 as male parent to get seed F0; B. auto-copulating for 5-7 generations and choosing at the same time plants with strong restoration and disease-resistant ability, short leaf, compact plant type and good appearance, cultivating out restoration system Zhenhui42 of new plant type, disease-resistant and high productivity; C. crossing sterile system II-32A with said restoration system Zhenhui42 and getting new rice breed II super 42 with high leaf blight-resistant property, short leaf and compact plant type. The rice got in this invention is characterized by the good comprehensive productivity, moderate maturity time; sound property; outstanding disease-resistant property and stable productivity.

The invention provides application of a gene which can improve the agricultural production traits of rice and improve the bacterial blight disease resistance and includes application of rice miR156 and a target gene of rice miR156 in improving rice resistance to the bacterial leaf blight. The invention also provides a method for cultivating anti-bacterial leaf blight transgenic rice, which comprises the following steps of: constructing an over-expression vector containing OsSPL7 or OsSPL14, transforming the constructed over-expression vector into rice, and up-regulating the expression of riceOsSPL7 or IPA1 / OsSPL14 to obtain the bacterial blight resistant transgenic rice; or down-regulating the expression of miR156 in rice crops so as to up-regulate miR156 target genes OsSPL7 and OsSPL14 (IPA1) to obtain the bacterial blight resistant transgenic rice. The method provided by the invention, on the condition of not affecting growth and development and improving the agronomic traits such as rice plant type and panicle type, takes microRNA as a starting point, provides a gene resource that affects rice plant type and panicle type, and also enhances resistance of rice to bacterial blight.

The invention relates to a cultivation method of a high-grain weight excellent-quality ternary hybrid rice variety, belonging to the technical field of plant breeding. The cultivation method comprisesthe following steps: using strong excellent-quality restorer line Zhenhui 084 as a male parent and Zhenhui 129 as a female parent for hybridization with groups; after inbred selection, adopting a wide-narrow-row high-fertilizer sparse planting culture mode from an F3 generation to carry out bacterial blight inoculation identification and screen high-grain weight and appearance quality by generations; measuring and combining the F3 generation with II-32A to identify a restoration degree; measuring and combining an F5 generation with an excellent-quality male sterile line Tianfeng A; culturinga strong excellent-quality restorer line 1126 with excellent plant types, disease resistance, high grain weight, high restoration degree and excellent rice quality; and producing hybrid seeds by the excellent-quality male sterile line Tianfeng A and Yanhui 1126 to obtain a new ternary hybrid rice variety 1126 with compact plant types, strong disease resistance, stable high production, high grain weight and high rice quality reaching an international excellent-quality rice level.

The invention discloses application of a rice auxin transport protein gene OsPIN9 in gene engineering, belonging to the field of gene engineering. The nucleotide sequence of the rice auxin transport protein gene OsPIN9 and the amino acid sequence for expressing OsPIN9 protein have an accession number of AK059229 in an NCBI website (www.ncbi.nlm.nih.gov / ). The application of the rice auxin transport protein gene OsPIN9 in gene engineering is reported for the first time in rice; the rice auxin transport protein gene OsPIN9 is involved in the transport of auxin in both aspects of heterogenous frog eggs and rice, thus increasing rice tillers, biomass, nitrogen utilization rate and final yield.

The invention relates to a salt-tolerant growth-promoting bacterium strain D5-2. The salt-tolerant growth-promoting bacterium strain D5-2 is preserved in the China General Microbiological Culture Collection Center on the preservation date of 5 May 2019 with the preservation number being CGMCC NO.17706, and the 16S rDNA nucleotide sequence of the salt-tolerant growth-promoting bacterium strain D5-2is as shown in SEQID NO.1. When the strain D5-2 is inoculated, under salt stress, the salt-tolerant growth promoting capacity of solanum lycopersicum can be notably improved. The strain is prepared into a microbial inoculum, and then the microbial inoculum is injected into rhizosphere soil of solanum lycopersicum seedlings, so that the plant type, the plant height and the stem thickness of the solanum lycopersicum can be obviously enlarged, the dry matter accumulation of the solanum lycopersicum seedlings can be notably promoted, the microbial inoculum has promoting effects on the root length, the surface area, the root volume and the number of root tips of the solanum lycopersicum seedlings, a salt injury phenomenon is alleviated, the growth of the solanum lycopersicum seedlings is promoted, and increase of salt tolerance of the solanum lycopersicum is facilitated.

The invention discloses a rice leaf rolling-associated protein OsMYB103L as well as an encoding gene and application thereof. The protein provided by the invention is (a) or (b): (a) the protein is composed of the amino acid sequence shown in the sequence 1 in a sequence table; (b) the protein is associated with the plant leaf rolling character and derived from the sequence 1 through carrying out the substitution and / or deletion and / or addition of one or several amino acid residues on the amino acid sequence of the sequence 1. When the rice leaf rolling-associated gene is over-expressed, the rice leaves roll inwards, but the characters such as fertility and the like of the transgenic rice are not significantly influenced and no other undesirable agronomic characters are observed, so that a significant potential function is provided to further improve the rice yield. The gene disclosed by the invention and the encoded protein thereof play significant roles in cultivating the plant varieties with properly rolled leaves and improving the plant types.

The invention provides an early ripening maize high-yield cultivation technology, belongs to the field of agriculture, and especially relates to a maize cultivation technology. The invention provides the early ripening maize high-yield cultivation technology which is fast in grouting and full in particles. The early ripening maize high-yield cultivation technology is characterized by comprising the following steps: 1, processing seeds and selecting seeds, pelleting seeds, using a seed coating to coat, to prevent and control pest and disease damages; 2, sowing and conserving seedlings: (1) ensuring soil moisture and sowing in proper moisture in soil, appropriately sowing early, on 5-10 cm land, when ground temperature being constant at 8 DEG C, sowing, to prevent sowing too late; 3, applying fertilizer and irrigating, according to target yield and foil fertility, determining fertilizer and water safeguard measures, to strictly guard against drought, especially paying attention to moisture supply in a critical period; 4, controlling pests and diseases later, in a belltop stage, using 10 ml chlorantraniliprole.thiamethoxam, 15 ml Ainong, and 30 kg water in each mu, spraying on stem leaves, to prevent European corn borers, oriental armyworms, aphids, and leaf spot diseases, rust disease, and gall smut, etc.

The invention discloses a histone demethylase gene JMJ703 for regulating a rice stem height, and applications thereof. According to the present invention, one of histone demethylases and gene JMJ703 thereof are selected through searching in a chromatin protein database, wherein the full-length cDNA accession number is AK121381, a gene ID number is 4338043, a prediction coding region comprises 3717 bp, and 1238 amino acids are coded; a RNA interference vector of the gene is transformed into rice through a RNA interference method, and results show that a stem height of the transgenic plant is shorter than a stem height of the wild type while a length of the leaf has no apparent change so as to improve a column shape of the rice; tobacco heterogenesis in vivo test results show that JMJ703 protein is demethylase of histone H3K4me1 / H3K4me2 / H3K4me3; and the JMJ703 is functional histone demethylase gene and can be provided for regulating a stem elongation process of the rice, wherein the stem of the rice expressed after gene inhibition becomes short and a characteristic of lodging resistance is provided while developments of leaves and other organs are normal so as to provide a selective plant type for breeding.

The invention belongs to the field of plant gene engineering and particularly relates to an application of a paddy rice miR160b gene in regulation and control on tillering angle. Through over-expression of the miR160b gene, a normal paddy rice plant has a phenotypic change of increased tillering angle, and through inhibition of the miR160b gene, the mutant phenotype of the over-expressed miR160b plant is recovered. The miR160b gene has the function of regulating and controlling plant type of paddy rice, wherein a stem-loop structure DNA sequence of the gene is represented as the SEQ ID No.1, a stem-loop structure precursor RNA sequence formed after transcription is represented as the SEQ ID No.2, and the sequence of a core region in mature miR160b formed after transcription and cutting is represented as the SEQ ID No.3. The invention discloses an expression mode of changing the paddy rice miR160b gene or the genes in other members of the family through gene engineering technology, so that the tillering angle of the paddy rice is regulated and controlled to form an ideal plant type, thereby increasing yield.

The invention discloses a method for constructing a pepper mutant library from ethylmethane sulfonate. The method is characterized in that a Zunla 1 is used as a mutagenesis object; on the premise ofpointing out the influence of treating fluid dose and excluding space of each seed on germination percentage, semi-lethal dose is determined by comparing the germination percentage of pepper seeds with EMS treating fluid with different concentrations at different mutagenesis time; the pepper seeds are treated by mutagenesis of the semi-lethal dose; mutation frequency and mutation types of M2 generation are investigated, a mutant capable of stably inheriting of M4 generation is identified and a mutant library is constructed; the mutation types of leaves, stems, fruits, growth period, flower organs, fertility and the like are obtained and create abundant materials for functional genomics reach of pepper; and meanwhile, partial beneficial mutation can be directly applied to breeding practice.

The invention belongs to the technical field of paddy rice breeding and discloses a paddy rice breeding method. According to the paddy rice breeding method, an imported foreign material is hybridizedwith a local variety; after different generations of separation and selection are carried out, properties are listed as follows: a combination (1): IR71121-35-1-1-1-2 / V13 hybridized F4 has the plant height of 80 cm, the quantity of total grains per ear is 246.7 and the fruiting rate is 73.4 percent; a combination (2): IR64446-2 / Dianjingyou No. 5 hybridized F5 has the plant height of 90 cm, the quantity of total grains per ear is 189.0 and the fruiting rate is 93.5 percent; a combination (3): Taebongbyeo / Undong 31 / / Yujing No. 8 hybridized F3 has the plant height of 90 cm, the quantity of totalgrains per ear is 203.0 and the fruiting rate is 91.0 percent. The paddy rice breeding method provides abundant paddy rice breeding resources for scientific researches and variety improvement of riceculture; the intra-regional and inter-regional genetic diversity is further expanded and a new leap of the production of paddy rice is realized.

The present invention discloses a breeding method of Zhendao 13 by step polymerization improvement technique, the rice bred by the method has the advantages of excellent quality, good taste, good rice marketability, outstanding disease resistance, good productivity and yield stability, harmonious ear-granule structure, self-adjusting ability, nice plant type and good maturing phase. The method described by the present invention comprises the following steps: A) selecting Japanese variety Guandong 135 as maternal plant to hybridize with Zhendao 2, obtaining Zhendao 72 after 4 generations inbred; B) selecting Japanese variety tsukinohikai as maternal plant to hybridize with Wuxiangjing 1, obtaining Zhendao 5088 after 4 generations inbred; C) selecting Zhendao 5088 as maternal plant to hybridize with Zhendao 72, obtaining 70 F0 seeds; D)seeding the F0 after seed soaking and pregermination, then selecting Wuyunjing 8 as maternal plant to perform multiple crossing, obtaining new rice variety namely Zhendao 13 having the advantages of high yield, good quality, stripe rust resistance and rice blast resistance.

Provided is the use of rice aerenchyma formation key gene OsLSD2 (Oryza sativa lysine-specific demethylase-2). The gene, with the accession number being AK111759,can be used in improving height, nitrogen use efficiency and / or production of plants. Specifically, prepared is rice with OsLSD2 gene overexpressed in roots and leaves by genetic method, with the root aerenchyma of rice plant changed. Compared with wild-type, the transgenic plants are higher,and the nitrogen use efficiency and single plant production increase by at least 30%.

The invention relates to a method for plant breeding and belongs to the field of genetic breeding. With the improvement of the living standards of people, adjustment of the planting structure and fierce competition in international markets, the problem of how to breed high-yield conventional japonica rice varieties which are high in disease resistance performance and high in taste quality is urgent to be solved. By the method for breeding new rice varieties through irradiation, defects in the prior art can be overcome, and the new rice varieties which are high in quality (high in both exterior quality and taste quality), high in yield and high in disease resistance can be bred in a short time.

The invention discloses a relative gene and coded protein and application of rice and strain type, wherein the cDNA sequence of gene comprises the following sequences: 1) DNA sequence in the SEQ ID No:1; 2) DNA sequence in the SEQ ID No:2; 3) DNA sequence with 90% isogenesis corresponding to SEQ ID No:1 with modified plant strain nucleotide sequence; 4) crossing nucleotide sequence to limit DNA sequence in the SEQ ID No:1 under high strict condition.