146 results about "Functional validation" patented technology

Expressed Sequence Tags (ESTs) isolated from soybean are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

The invention relates to the technical field of rice gene engineering. Application of miR164 small RNA of rice for controlling development and fertility of a root system of a plant to genetic improvement of the rice is obtained through separation, cloning and functional verification, and the gene is one of the following nucleotide sequences: a DNA sequence shown in a sequence table SEQ NO:1 and an RNA sequence shown in SEQ NO:2. After connecting with an exogenous promoter, a nucleotide sequence containing miR164 precursor is transferred into the rice, the root system of the transgenic rice is developed and sterile, and the fertility can be recovered by externally applying hormone.

Expressed Sequence Tags (ESTs) isolated from maize are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

Expressed Sequence Tags (ESTs) isolated from cotton are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

The invention provides a cloning and transient expression method of persimmon deastringency related genes. The method comprises: first acquiring gene 3'-terminal sequences, then employing 3' RACE (rapid amplication of cDNA ends) technology to acquire 3'-terminal sequences of ADH and PDC gene family members in persimmon fruits; adopting Q-PCR (quantitative polymerase chain reaction) technology to analyze the genetic expression of ADH and PDC gene family members in a persimmon fruit deastringency treatment; and conducting separation to obtain full-length sequences of ADH and PDC gene family members, then establishing a transient expression system of persimmon leaves. The method of the invention clones the persimmon deastringency related gene family members, analyze the expression patterns of the family members in the persimmon deastringency treatment, and finally verifies functions of the target genes through transient expression of the persimmon leaves. The invention clarifies the regulation mechanism for postharvest persimmon deastringency from the molecular mechanism aspective, and can be used for further functional verification (transgenosis, gene interaction and the like) study of the postharvest persimmon deastringency mechanism.

The disclosed method discloses embedded type software application architecture (ETSAA) oriented to instrument equipment in multimedia category. The method is established based on a set of embedded type software application component library oriented to digitalized instrument equipment in multimedia category, especially a multimedia dedicated component library. The invention realizes service function flow of multimedia application system, and provides audio and video codec with powerful functions. In the method, a platform installation of embedded system of instrument equipment in multimedia category provides tools and environment support for fast developing. Since the said ETSAA oriented to instrument equipment in multimedia category is passed the functional validation, the invention shortens development cycle greatly, and reaches standardization, modularization, and reusability of system software.

Expressed Sequence Tags (ESTs) isolated from cotton are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

The invention belongs to the technical field of rice genetic engineering, and in particular relates to gene OsHSF08 capable of remarkably increasing drought resistance during seeding stage and booting stage, which is obtained through screening, separating, cloning and functional verification. The gene OsHSF08 capable of controlling rice drought resistance character is obtained through cloning by screening insertion of T-DNA into rice mutant library, and has nucleotide sequence shown in SEQ ID No: 1; and the coded protein has nucleotide sequence shown in SEQ ID No: 2. Coseparation detection shows that the mutant is tightly linked with drought sensitivity phenotype, and the over-expression of OsHSF08 gene in rice can remarkably increase the drought resistance of the genetically modified rice, so that new function and new application approach of the gene are proven.

A system, apparatus, computer program product and method of performing functional validation testing in a system are provided. Generally, functional validation testing includes data acquisition and data validation testing. During the functional validation testing two devices may be exchanging data. The exchange of data by the two devices may be referred to as data acquisition. The data from one device and the data from the other device may be compared to each other. This may be referred to as data validation. When data is exchanged during data acquisition, it is also stored in appropriate locations in a pool of buffers in memory. During the data acquisition, checks are made to determine if the system is entering an idle cycle. If so, the data validation test is performed by using the data in the pool of buffers in memory.

Expressed Sequence Tags (ESTs) isolated from soybean are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.