49 results about "Gene interaction" patented technology

The present invention provides protein fragment complementation assays for drug discovery, in particular to identify compounds that activate or inhibit cellular pathways. Based on the selection of an interacting protein pair combined with an appropriate PCA reporter, the assays may be run in high-throughput or high-content mode and may be used in automated screening of libraries of compounds. The interacting pair may be selected by cDNA library screening; by gene-by-gene interaction mapping; or by prior knowledge of a pathway. Fluorescent and luminescent assays can be constructed using the methods provided herein. The selection of suitable PCA reporters for high-throughput or high-content (high-context) assay formats is described for a diversity of reporters, with particular detail provided for examples of monomeric enzymes and fluorescent proteins. Methods are described for constructing such assays for one or more steps in a biochemical pathway; testing the effects of compounds from combinatorial, natural product, peptide, antibody, nucleic acid or other diverse libraries on the protein or pathway(s) of interest; and using the results of the screening to identify specific compounds that activate or inhibit the protein or pathway(s) of interest. Single-color and multi-color assays are disclosed. Further disclosed are universal expression vectors with cassettes that allow the rapid construction of assays for a large and diverse number of gene / reporter combinations. The development of such assays is shown to be straightforward, providing for a broad, flexible and biologically relevant platform for drug discovery.

The invention provides a cloning and transient expression method of persimmon deastringency related genes. The method comprises: first acquiring gene 3'-terminal sequences, then employing 3' RACE (rapid amplication of cDNA ends) technology to acquire 3'-terminal sequences of ADH and PDC gene family members in persimmon fruits; adopting Q-PCR (quantitative polymerase chain reaction) technology to analyze the genetic expression of ADH and PDC gene family members in a persimmon fruit deastringency treatment; and conducting separation to obtain full-length sequences of ADH and PDC gene family members, then establishing a transient expression system of persimmon leaves. The method of the invention clones the persimmon deastringency related gene family members, analyze the expression patterns of the family members in the persimmon deastringency treatment, and finally verifies functions of the target genes through transient expression of the persimmon leaves. The invention clarifies the regulation mechanism for postharvest persimmon deastringency from the molecular mechanism aspective, and can be used for further functional verification (transgenosis, gene interaction and the like) study of the postharvest persimmon deastringency mechanism.

A method of introducing a molecule of interest into a plant cell having a cell wall includes interacting a gamma-zein peptide with a molecule of interest to form a gamma-zein linked structure. The gamma-zein linked structure is then placed in contact with the plant cell having a cell wall, and allowing uptake of the gamma-zein linked structure into the plant cell. Alternatively, a gene of interest can be expressed in a plant cell having an intact cell wall by interacting a gamma-zein peptide with the gene of interest to form a gamma-zein linked gene structure, allowing uptake of the gamma-zein linked gene structure into the plant cell, and expressing the gene of interest in the plant cell and its progeny.

The invention provides a cloning and transient expression method of persimmon deastringency related genes. The method comprises: first acquiring gene 3'-terminal sequences, then employing 3' RACE (rapid amplication of cDNA ends) technology to acquire 3'-terminal sequences of ADH and PDC gene family members in persimmon fruits; adopting Q-PCR (quantitative polymerase chain reaction) technology to analyze the genetic expression of ADH and PDC gene family members in a persimmon fruit deastringency treatment; and conducting separation to obtain full-length sequences of ADH and PDC gene family members, then establishing a transient expression system of persimmon leaves. The method of the invention clones the persimmon deastringency related gene family members, analyze the expression patterns of the family members in the persimmon deastringency treatment, and finally verifies functions of the target genes through transient expression of the persimmon leaves. The invention clarifies the regulation mechanism for postharvest persimmon deastringency from the molecular mechanism aspective, and can be used for further functional verification (transgenosis, gene interaction and the like) study of the postharvest persimmon deastringency mechanism.

The invention discloses the application of rice tRNA isopentenyl transferase gene OsIPT9 in rice resistance to brown planthopper. It belongs to the field of plant genetic engineering technology. OsIPT9 It has the amino acid sequence shown in SEQ ID No.1, the nucleotide sequence shown in SEQ ID No.2, and the ORF sequence shown in SEQ ID No.3. Through Agrobacterium-mediated genetic transformation, the OsIPT9 Transplanted into Nipponbare, which is susceptible to N. lugens, the overexpressed plants have enhanced resistance to N. lugens; at the same time, the resistant material NIP‑Bph6‑NIL was knocked out by CRISPR / Cas9 technology OsIPT9 , resulting in a significant down-regulation of plant resistance to N. lugens. The gene of the invention provides a good theoretical basis for studying the gene interaction between rice and brown planthopper, and has reference significance for studying gene molecular function and breeding.