548 results about "Cell metabolism" patented technology

The present invention includes 1) a novel formulation for the treatment of hair loss comprising oleanolic acid (a 5α-reductase inhibitor), apigenin (a vasodilator), and biotinyl-GHK (a cell metabolism stimulant), 2) a novel additive for the treatment of hair loss comprising oleanolic acid, apigenin, biotinyl-GHK and a delivery agent, 3) a personal care, cosmetic, and / or dermopharmaceutical composition comprising oleanolic acid, apigenin, biotinyl-GHK, and at least one additional ingredient, and 4) a method for treating hair loss comprising the administration of oleanolic acid, apigenin, and biotinyl-GHK.

Disclosed and claimed are: a composition including at least one glycosaminoglycan, e.g., CIS, at least one perfluorinated substance and at least one alginate, e.g., sodium alginate, wherein:the at least one glycosaminoglycan and / or the perfluorinated substance and / or the alginate are cross-linked or polymerized, e.g., the alginate is cross-linked or polymerized, for instance by addition of an inorganic salt, such as a calcium salt; orthe at least one glycosaminoglycan, the perfluorinated substance and the alginate are covalently bound, e.g., by means of a coupling reaction involving a linker molecule such as DVS; orthe at least one glycosaminoglycan and / or the perfluorinated substance and / or the alginate are cross-linked or polymerized, e.g., the alginate is cross-linked or polymerized, for instance by addition of an inorganic salt, such as a calcium salt, and the at least one glycosaminoglycan and the alginate are covalently bound, e.g., by means of a coupling reaction involving a linker molecule such as DVS, and the covalent binding can have been performed prior to cross-linking or polymerizing or vice versa; and,gels comprising the composition; mixtures of such gels or of at least one such gel and at least one such composition; and,methods for making and using such compositions and gels, including products therefrom such as "paints", sprays, matrices, beads, microcapsules.

Treatment of human hepatocytes with farnesoid X receptor (FXR) agonists resulted in increased expression of FGF-19. Methods of using FXR agonists to alter cell metabolism, and in pharmaceutical weight loss methods, are described.

A skin care composition is useful for augmenting cellular metabolism in skin cells and thereby enhancing the regulation of intracellular signaling. The composition comprises a primer for skin cell mitochondrial function, such as a Krebs cycle intermediate, a precursor of a Krebs cycle intermediate, salts or esters thereof, or combinations thereof. The composition may also include antioxidants for free radical regulation and a pharmaceutically acceptable topical vehicle, such as an emollient base for skin health. A method for stimulating the mitochondrial activity of skin cells comprises administering to the skin of a person in need thereof a composition as described herein.

A method of diagnosing Alzheimer's disease (AD) and Mild Cognitive Impairment (MCI) is disclosed which was identified by the fact that an increased level of iron regulating protein-2 (IRP-2) was identified in Alzheimer's patients. Further, diseases of increased cellular metabolism such as cancer showed high levels of both IRP-2 and transferrin receptor. This suggests that, in diseases of increased cellular metabolism, both aspects of iron accumulation are highly expressed, while in Alzheimer's disease only IRP-2 is highly expressed. From these results, a non-invasive test for Alzheimer's disease may be produced using patient samples containing peripheral blood cells and identifying the level of expression of IRP-2 and Transferrin receptor. Those patients which: 1. over-express IRP-2 as compared with normal controls, and 2. have comparable levels of Transferrin receptor expression to normal controls, can be identified as having or prone to AD and MCI. Further, it can be envisioned that this may be used for further diagnosis and staging of cancers of the blood.

The invention discloses an artificial flexible-posture preserved ornamental plant and a processing method thereof. The artificial flexible-posture preserved ornamental plant is prepared from a naturally grown living herbaceous plant or woody plant which is harvested, kept natural posture, subjected to disinfection, fresh-keeping and coloring treatment, and then used as an ikebana material. The processing method comprises that: after pretreated, a living flowering plant material is soaked by a flexible-posture plant treating agent prepared from carbohydrate, a nourishment regulator, an antioxidant, a humectant, a preservative, edible pigment and water in a soaking container at an environmental temperature of between 15 and 22 DEG C and a relative humidity of between 70 and 80 percent untilleaves on the top of the plant are evenly dyed; and the soaked plant is aired, dried, graded and shaped into the artificial flexible-posture preserved flower material. The method utilizes the naturalmechanism of cell metabolism of flower plants, performs cellular fluid displacement and cell enclosing treatment through the flexible-posture plant treating agent, and makes the plants keep natural postures, texture and flexibleness, not influenced by spacial environment, and preserved as long as 1 to 2 years. The artificial flexible-posture preserved ornamental plant taken as a bonsai flower material does not need water dressing or daily maintenance, has stronger enjoyment property than a dry flower, and prolongs the fresh-keeping time by hundreds of times than fresh cutting plants and flowers.

The invention relates to a novel measurement method and the device thereof, which combines the time-resolved self-fluorescence lifetime imaging technology and a laser scanning confocal ophthalmoscopeand realizes the early diagnosis of the ocular fundus diseases by obtaining and analyzing the self-fluorescence intensity and the service life information of the ocular fundus. The light which is emitted by a pulse laser is sent to the scanning head of the laser scanning confocal ophthalmoscope to excite the ocular fundus inner-source flgorgen, and the fluorescence which is emitted from the ocularfundus is collected by a time-correlated single photon counter or a high-repetition-rate synchronous scanning camera, so as to realize the service life measurement and the imaging of the self-fluorescence. As the service life of the self-fluorescence of the ocular fundus is very sensitive to the micro-environment and the cell metabolism state of flgorgen molecule and is not affected by the difference of the intensity of the flgorgen of the ocular fundus, the measurement of the fluorescence service life provides a brand new route for the detection of ocular fundus diseases and the obtainment of the function information of ocular fundus. The invention can become the solution and the basis of the early diagnosis of various ocular fundus diseases, which plays an important role in pathogeny research, clinical diagnosis, staging and typing, prognosis judgement, real-time observation and the analysis of the development of ocular fundus diseases, therefore the invention can be widely appliedin the field of medical research and clinical medical diagnosis.

The invention discloses preparation and application of human-stem-cell-secreted bioactive factors and a lysis solution. The process comprises the following steps of: extracting stem cells of the umbilical cord or the placenta tissue of a health human person; performing in-vitro culture and amplification; and preparing high-purity and high-activity stem-cell-secreted bioactive factors and bioactive substances in the stem cell (namely, high-purity stem cell lysis solution) by the modern biological technologies of low-temperature separation, purification and the like. According to the invention, the bioactive factors or the substances do not contain any animal component, and can be developed into a product with a cell biological activity; after acting on the human body, the product can activate the stem cell of the organism and repair or replace damaged, lesion and old cells; and the product has the functions of regulating the cell metabolism function of the organism, enhancing the immunity of the organism, delaying the aging and the like. The human bioactive factors or the substances have great application prospect in the fields of medical science, healthcare and beauty.

The embodiments herein disclose a method of fabricating composite scaffolds for skin tissue regeneration. The methacrylated hyaluronic acid (HAMA) and methacrylated gelatin (GelMA) are synthesized. The poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are synthesized. The hydrogel is synthesized. The composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds is fabricated. A plurality of physico-chemical characteristics of the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are analysed. The physico-chemical characteristics comprises mechanical properties, swelling ratio and enzymatic degradation and scanning electron microscope imaging. The fibroblast cells are encapsulated within the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds and hydrogels. The fibroblast cells are seeded on composite scaffold and PGS-PCL scaffold. The fibroblast cell viability, fibroblast cell attachment, fibroblast cell spreading, fibroblast cell proliferation and fibroblast cell metabolism are analysed in composite scaffolds, PGS-PCL scaffolds and hydrogels.

The invention relates to an anti-acne facial cream which comprises Chinese traditional medicine composition and substrate formulation; the Chinese traditional medicine composition at least contains five of the following ingredients, smilax glabra, coix seed, kudzu root, astragalus, chrysanthemum, dwarf lilyturf tuber, lucid ganoderma and giant knotweed rhizome; the Chinese traditional medicine composition contains at least five of the following ingredients by weight: 5-20% of smilax glabra, 5-40% of coix seed, 3-20% of kudzu root, 5-30% of astragalus, 3-35% of chrysanthemum, 3-40% of dwarf lilyturf tuber, 3-20% of lucid ganoderma and 3-20% of giant knotweed rhizome. The anti-acne facial cream has the advantages of preventing and treating the acne, quick effect, obvious effect, no pockmark, uneasy recurrence, being used for cosmetic whitening, having the functions of skin mildness, moisture retention and sunscreen, reducing the oil secretion and promoting the cell metabolism.

A biosensor for detecting a bioeffecting substance in a test sample includes a cell network of at least one electrically excitable cell provided on a substrate in a predetermined geometry with a predefined axonal / dendritic polarity. The at least one cell is capable of producing a signal in response to the presence of the bioeffecting substance. At least one signal transducer is provided with the substrate in a predetermined geometry and is capable of detecting the signal produced by the cell. A culture medium capable of supporting metabolism of the cell is also provided.

The invention discloses a facial mask with repairing and anti-allergic functions and a preparation method thereof, comprising component A and component B, component A comprising raw materials: propylene glycol, carbomer, glycerin, jojoba wax PEG‑120 esters, Transparent Xanthan Gum, Amino Acid Moisturizer, Sodium Hyaluronate, Hydrolyzed Hyaluronic Acid, Antioxidant and Deionized Water; Component B Contains Raw Materials: PH Regulator, PPG‑10 Methyl Glucose Ether, Glabridin, Citrus Peel Extract, EGF, Nano Gold, Soothing Repair Extract, Yeast Extract, Snail Secretion Filtrate Extract, Gentiana Extract, Carboxymethyl Dextran C90, 1,2‑Hexanediol, and Glacier Water. The invention improves cell metabolism speed and promotes the full-effect absorption of nutrition in skin care products. Nano gold has the functions of antibacterial, anti-inflammatory and anti-oxidation, helps skin firming, anti-wrinkle, anti-aging, can improve the activity of skin collagen, and makes the skin appear natural luster , Enhance skin anti-aging ability.

The invention discloses an artificial soft posture preserved ornamental plant treating agent. The treating agent comprises carbohydrates, a nutrition regulator, an antioxidant, a humectant, an antiseptic and food color, and is prepared from 30 to 45g of carbohydrates, 30 to 45g of nutrition regulator, 20 to 35g of antioxidant, 20 to 35g of humectant, and 12 to 25g of food color which are dissolved in 1 liter of water and uniformly mixed. The treating agent can perform cell sap replacement and cell sealing treatment by utilizing the natural mechanism of the cell metabolism of flowers and plants, so that the plants can keep natural postures, texture and softness, are not affected by space and environment, and can be kept fresh as long as 1 to 2 years; the plants, treated by the treating agent and used as bonsai flowers, are unnecessary to water and maintained daily; the ornamental characteristics of the plants are greatly enhanced compared with dry flowers; and the preservation period is prolonged by hundreds of times compared with that of fresh cut plants and flowers.

The invention relates to a microbial feed additive for promoting lactation and improving the quality of dairy products and a production method thereof, and is characterized in that raw materials of lactobacillus plantarum, streptococcus lacticus, lactobacillus casei, saccharomyces cereviseae, nutritive medium, sterile water, etc., are first treated with anaerobic fermentation; fermentation liquor are then mixed after centrifugation and blended with nutrition carriers evenly to be placed in a closed fermentation chamber for anaerobic fermentation and finally crushed into power after dried so as to obtain the finished products. The invention comprises a great deal of effective microorganisms, antioxidants of coenzyme, SOD and vitamins, etc., and physiological metabolins of amino acid and active peptides, etc. which can have effects of nutrifying and repairing cell and improving cell metabolism after entering the body of a cow so as to form sound ecological environment in the cow body, promote transformation and absorption of nutrient matters and immunity of the organism and even prevent garget of the cow and improve the ability of anti-heat stress and the quality of dairy products while improving the milk yield of cows.

The invention discloses fluorochrome for cytolysosome positioning and a preparation method and application thereof. The preparation method of the fluorochrome compound includes the steps that a benzophenone derivative containing methyl substituents is reacted under the catalysis of Zn / TiC14, an obtained product and N-bromosuccinimide are subjected to a bromination reaction, then a product is reacted with morpholine, and the tetraphenyl ethylene derivative stain containing morpholine groups is obtained. The preparation method is easy to implement, raw materials are easy to get, and reaction conditions are mild. The obtained fluorochrome contains tetraphenyl ethylene fluorescence groups capable of gathering the activity of inducing fluorescence emission and can be used for performing specific staining on all kinds of cytolysosome. The fluorochrome has the advantages of being small in cytotoxicity, stable in cell metabolism resistance, good in photobleaching resisting effect and the like and hardly affects normal physiological activities of cells, and a new method is provided for observing the morphologic changes of cytolysosome for a long time. The fluorochrome can be widely applied to monitoring the morphology of cytolysosome, observing the apoptosis process and the like.

The invention provides a model for generating predicted action potentials of an electrically active cell. The disclosed model includes three operatively coupled submodels. A first submodel contains Hodgkin-Huxley elements generating action potentials based on electrical equivalent circuits. A second submodel is based on reaction kinetics of cell metabolism and is operatively coupled with the first submodel. A third submodel is based on Boolean dynamics representing signaling and associated cellular processes and is operatively coupled with the first and second submodels. The invention includes storing a library of calculated action potentials and associated cellular parameters generated by the model, applying a stimulus to the electrically active cell in vitro so that the cell generates an action potential; and comparing the cell-generated action potential with those stored in the library, wherein a match is predictive of the cellular point of action of the applied stimulus according to the parameters stored.

The invention discloses a fibroblast liquid for beauty treatment and a preparation method thereof. The fibroblast liquid contains a cell culture solution, collagens, cell growth factors, amino acids and vitamins, wherein the cell growth factors comprise fibroblast growth factors, epidermal cell growth factors, hepatocyte growth factors and keratinocyte growth factors. The preparation method of the fibroblast liquid comprises the following steps: acquiring umbilical cord tissue hypodermal cells, carrying out primary culture of the umbilical cord tissue hypodermal cells, subcultring the umbilical cord tissue hypodermal cells, collecting the obtained cell culture liquid, and adding glycerin and cytokines to prepare the fibroblast liquid. The fibroblast liquid has the efficacies of epidermal cell metabolism promotion, skin elasticity enhancement, injured epidermal cell restoration, color spot removal and the like; and the preparation method is simple, is easy to operate, and is suitable for the large-scale growth preparation of stem cell liquids.

The invention relates to a whitening and freckle-removing skin care product capable of blocking, inhibiting and reducing melanin and a preparation method of the skin care product, in particular to a whitening and freckle-removing formula capable of blocking, inhibiting and reducing melanin. The formula comprises niacinamide, betaine, beta-glucan, a cynanchum atratum extract, a morus alba bark extract, coated saccharomyces fermentation product filtrate, a spirulina platensis extract, a chlorella vulgaris extract, an oryza sativa germ extract, a hordeum vulgare germ extract, a triticum vulgare germ extract and the like. The skin care product can block, inhibit and reduce melanin, accelerate cell metabolism, accelerate falling of melanin keratinocyte, eliminate skin aging, inhibit, delay andreduce wrinkles and have skin conditioning functions of promoting skin regeneration. The skin care product is not limited by product dosage forms, can be added to aqueous solutions, emulsions, cream,essence and the like, can have multiple purposes and makes up the obvious defect that commercial products are single in function.

The invention provides cell preservation liquid. The cell preservation liquid is prepared from the following components: 30 to 120mM of an osmotic pressure stabilizer, 2 to 6mM of a saccharide substance, 0 to 10mM of a reducing agent, 0 to 120mM of an electrolyte solution and 25 to 60mM of an amino acid; the osmotic pressure of the cell preservation liquid is 310 to 350mOsm / L. The cell preservation liquid provided by the invention can be used for effectively reducing cell metabolism and reducing low-temperature injuries under a low-temperature condition; living cells are convenient to preservein a transportation process, and functional active cells are also conveniently stored for a short period by an organization.

A mitochondria targeted gold nanoparticle (T-3-BP-AuNP) decorated with 3-bromopyruvate (3-BP) and delocalized lipophilic triphenylphosphonium (TPP) cations to target the mitochondrial membrane potential (Δψm) was developed for delivery of 3-BP to cancer cell mitochondria by taking advantage of higher Δψm in cancer cell compared to normal cells. This construct showed remarkable anticancer activity in prostate cancer cells compared to non-targeted construct NT-3-BP-AuNP and free 3-BP. Anticancer activity of T-3-BP-AuNP was further enhanced upon laser irradiation by exciting the surface plasmon resonance band of AuNP and thereby utilizing a combination of 3-BP chemotherapeutic and AuNP photothermal effects. T-3-BP-AuNPs showed markedly enhanced ability to alter cancer cell metabolism by inhibiting glycolysis and demolishing mitochondrial oxidative phosphorylation in prostate cancer cells. Our findings demonstrated that mitochondria targeted and concerted chemo-photothermal treatment of glycolytic cancer cells with a single NP may have promise as a new anticancer therapy.

The invention discloses the use of a composition comprising human growth hormone and human epidermal growth factor in cosmetics and provides a production process thereof. The invention uses human growth hormone in cosmetics together with human epidermal growth factor by using biotechnology. The human growth hormone and the human epidermal growth factor has synergistic effects in supplementing bioactive factors for promoting epidermal cell metabolism and proliferation, thereby basically improving the physiological status of skin, promoting repairing and metabolism of epidermal tissues, promoting extracellular synthesis of biomacromolecules such as hyaluronic acid and glycoprotein, promoting skin tendering and rejuvenation, relieving color spots, reducing wrinkles, and resisting aging. The inventive composition has remarkably cosmetic effect and has the advantages of good safety and reliability, and no adverse side effects.

A device and system that allows an inverted microscope with a movable x-y stage to also function as a time-resolved plate reader. The device, which is sized to fit into the condenser holder of an inverted microscope, not only provides plate-reading function at very low cost compared to that of purchasing a plate reader, but it also allows the correlation of plate reader measurements with images of a specimen. The device and system is also able to measure other parameters associated with cell metabolism, such as pH changes.

The invention discloses an astaxanthin mask having an oxidation-resistant effect. The astaxanthin mask is characterized by being prepared from, by weight, 5-15 parts of glycerol, 5-10 parts of honey, 5-15 parts of aloe vera extracting liquid, 2-10 parts of lemon oil, 5-10 parts of arbutin, 2-5 parts of astaxanthin, 4-10 parts of hydroxypropyl-beta-cyclodextrin, 2-5 parts of ethyl alcohol, 0.01-0.1 part of methylparaben and 30-45 parts of distilled water. The astaxanthin is entrapped in the hydroxypropyl-beta-cyclodextrin, so that the solubility of the astaxanthin in mask essence is improved. The astaxanthin mask has the very good oxidation-resistant effect, can effectively remove facial oxygen free radicals and promote cell metabolism, and makes the skin rejuvenated. In addition, the astaxanthin mask also has the effects of whitening, moisturizing, minimizing pores and the like, and keeps the skin exquisite, moisturized and smooth.

The invention discloses a double-layer skin caring emulsion and a preparing method thereof, and aims at providing an emulsion which can promote cell metabolism and is good in moisturizing effect. The emulsion is mainly prepared from disodium EDTA, polyglutamic acid, glycerol, polyethylene glycol-400, dipropylene glycol, PEG-40 hydrogenated castor oil, sodium myristoyl glutamate, PPG-25-laureth-25, cyclopentasiloxane, hydrogenated polydecene, tocopheryl acetate, caprylic / capric triglyceride, phytosteryl / octyldodecyl laupoyl glutamate, hydrogenated polyisobutene, butyrospermum fruit grease, radix scutellariae root extract, yellowish sophora root extract, glycyrrhiza inflata batal extract, verbena extract, lactobacillus / eriodictyon californicum fermentation product extract, phenoxyethanol / ethylhexylglycerin, essence and the balance deionized water. The double-layer skin caring emulsion and the preparing method belong to the technical field of cosmetics.

The invention discloses a skin base liquid having a micro-ecological balance self-repairing function. The skin base liquid comprises, by weight, 0.05-0.1% of sodium hyaluronate, 0.05-0.2% of carbomer, 0.05-0.2% of triethanolamine, 0.05-0.1% of disodium ethylenediaminetetraacetate, 1.5-2.5% of lubrajel oil, 0.05-0.1% of methyl p-hydroxybenzoate, 2-3% of apple fruit cell culture extract, 0.5-1% of glycerol glucoside, 2-3% of ganoderma lucidum extract, 2-5% of palmitoyl tripeptide-5, 2-5% of alpha-arbutin, 0.5-1% of dendrobium officinale extract, 0.5-1% of caprylhydroxamic acid and 0.5-2% of azone. The skin base liquid can effectively repair cell damage, promote cell metabolism, make dark and uneven color skin bright and moist and make skin fine, soft and youthful.

The invention discloses a wearable physiotherapy device with functions of emotion and sleep adjustment and skin care. The wearable physiotherapy device comprises a transverse beam and two side beams,and the two ends of the transverse beam are connected with the two side beams through corner connecting parts; the transverse beam is provided with an air fresher chamber, the tail ends of the two side beams are provided with hanging lugs and micro audio playing modules respectively, and the two corner connecting parts are provided with upper covers and light-emitting panels; the upper covers, thelight-emitting panels and the corner connecting parts define a light-emitting part mounting space, and the light-emitting part mounting space is internally provided with an LED light set, a first-stage light reflecting panel and a second-stage light reflecting panel. According to the wearable physiotherapy device, the personalized sleep adjustment can be achieved by utilizing sound, light rays and odor, and therefore the life quality of a user is significantly improved; the light rays adopted for phototherapy can be set to have specific wave bands, so that the device has the function of promoting blood circulation and cell metabolism and proliferation to achieve the effect of skin care and beautifying; meanwhile, through the combination of the emotion adjustment function of the phototherapy, the product has higher use value.

The invention relates to acne-removing refine whitening cream, comprising traditional Chinese medicine composition composed of the following components by weight percent: 5-15% of rhizoma smilacis glabrae, 5-40% of semen coicis, 3-15% of kudzu-vine root, 5-20% of astragalus root, 3-25% of chrysanthemum, 3-40% of radix ophiopogonis, 3-10% of lucid ganoderma and 3-10% of giant knot weed; the extracts of the traditional Chinese medicine components are matched with appropriate ointment, thus obtaining the finished product. The invention is used for preventing and treating whelk, has fast action, obvious effect, no acne trace after healing and difficult recrudescence, is used for whitening skin and has multifunction of tendering skin, moisturizing and explosion proofing, reducing oil secretion and promoting cell metabolism.

A night maintenance mask solution is made through dipping a liquid in mask paper, and 1000 parts of the liquid are prepared from, by weight, 0.5-3 parts of micro-molecular hyaluronic acid, 1-5 parts of an aloe extract product, 0.7-9 parts of propylene glycol, 5-50 parts of 1,2-butylene glycol, 5-30 parts of glycerin, 20-50 parts of a ginseng extract liquid, 10-50 parts of a licorice root extract liquid, 5-20 parts of rose hydrolat, 1-10 parts of ethyl ascorbic acid, 0.5-5 parts of silk peptides, 0.5-5 parts of argireline peptides, 0.5-5 parts of apple stem cells, and the balance of water. The night maintenance mask solutionis aqueous, can rapidly enter skins, can remove wrinkles, has water supplementation, water retention, restoration, nutrition supplementation, anti-inflammation, bacterium prevention, itch relief and anti-allergy effects in skins, participates in and promotes cell metabolism, relieves skin wrinkles, enhances the skin elasticity, also has the advantages of no irritation to skins and no abnormal sensation, long action time and substantial effect, and is suitable for all skin types.

A method for preparing a three-dimensional cell scaffold for in-vitro toxicological evaluation of tobacco products is characterized in that an interconnected and porous material provided with a polymer is obtained through high-internal-phase emulsion polymerization in a cell culture dish, the material has large holes of the size of cells for in-vitro toxicological evaluation of tobacco products, the holes are communicated with one another, thereby facilitating input of oxygen and nutrients required for cell growth and output of cell metabolism waste, and the material surface is modified, so that the material has good cell affinity. Compared with existing panel type monolayer cell culture methods commonly adopted for toxicological evaluation, the method has the advantages that cells can realize three-dimensional growth on the scaffold and have more closer three-dimensional shape and biochemical and functional properties as compared with that originally in vivo. Therefore, when the cellscultured by use of the three-dimensional cell scaffold are used for in-vitro toxicological evaluation of the tobacco products, the evaluation result is more objective and real. The cell scaffold is prepared in the culture dish and is more convenient for following experiments.

The invention discloses a moisturizing cosmetic composition and application thereof. The moisturizing cosmetic composition comprises barley seed extract, almond extract, aloe extract C14-22 alcohol / C12-20 alkyl glucoside, glyceryl stearate / PEG-100 stearate, cetearyl alcohol, squalane, polydimethylsiloxane, caprylic / capric triglyceride, avocado tree fruit grease and sodium hyaluronate. Natural plants are matched according to a specific proportion, and various functional ingredients are mutually coordinated and synergistic, so that the moisturizing cosmetic composition can effectively promote cell metabolism, soften cuticle, moisten skin, inhibit tryosinase, brighten skin color and nourish nutrition, has comprehensive nursing effect and has more remarkable moisturizing effect especially.