82 results about "Plate reader" patented technology

An instrument is disclosed for fluorescence assays which is capable of reading many independent samples at the same time. This instrument provides enhanced throughput relative to single-sample instruments, and is well-suited to use in general fluorescence, time-resolved fluorescence, multi-band fluorescence, fluorescence resonance energy transfer (FRET), and fluorescence polarization. This invention is beneficial in applications such as high-throughput drug screening, and automated clinical testing. Also disclosed are means and methods for a fluorescence polarization measurement which is highly sensitive, inherently self-calibrated, and unaffected by lamp flicker or photobleaching. This fluorescence polarization invention can be practiced on a variety of fluorescence instruments, including prior-art equipment such as microscopes and multi-well plate readers.

A multi-modality fluorescence reference plate comprising wells coated with a fluorogenic compound is described, together with a method of producing such a plate. The plate has utility for calibrating fluorescent plate readers and imaging systems for measuring steady-state fluorescence, time-resolved fluorescence, fluorescence lifetime and / or fluorescence polarisation.

This invention discloses a system and method useful in moving labware between laboratory devices, such as liquid handlers, readers, washers, dispensers, sealers, incubators, microarrayers and labeling devices, such a bar code labelers, to aid in automation of laboratory tasks and assay procedures. Work cells addressing particular laboratory tasks comprise modular components such as stack links, track links, arm links and laboratory devices, such as washers or plate readers. Based upon the required flow of labware movement, the individual modular components are selected and interconnected to create a high-speed work cell. The work cells are easy to configure and setup and permit configuration on available bench top space or within other small spaces, such as laboratory fume hoods. All mechanical and electrical connections between the module components of the work cell are self-contained within the system. A mechanically robust and simple design of the work cell configuration permits reliable walk away automation.

An instrument is disclosed for fluorescence assays which is capable of reading many independent samples at the same time. This instrument provides enhanced throughput relative to single-sample instruments, and is well-suited to use in general fluorescence, time-resolved fluorescence, multi-band fluorescence, fluorescence resonance energy transfer (FRET), and fluorescence polarization. This invention is beneficial in applications such as high-throughput drug screening, and automated clinical testing. Also disclosed are means and methods for a fluorescence polarization measurement which is highly sensitive, inherently self-calibrated, and unaffected by lamp flicker or photobleaching. This fluorescence polarization invention can be practiced on a variety of fluorescence instruments, including prior-art equipment such as microscopes and multi-well plate readers.

Methods for concurrently processing multiple biological chip assays by providing a biological chip plate comprising a plurality of test wells, each test well having a biological chip having a molecular probe array; introducing samples into the test wells; subjecting the biological chip plate to manipulation by a fluid handling device that automatically performs steps to carry out reactions between target molecules in the samples and probes; and subjecting the biological chip plate to a biological chip plate reader that interrogates the probe arrays to detect any reactions between target molecules and probes.

An intelligent parking management system for residential communities is disclosed that includes a license plate reader; and a server communicatively coupled to the license plate reader over a network. The server includes a memory storing a parking policy and registered license places for one or more residential communities registered with the at least one server; and at least one processor. The processor is operably configured to receive a license plate number, over the network, from the license plate reader; compare the license plate number to a plurality of registered license plate numbers stored in the memory; and communicate, over the network, a parking violation message as a result of determining that the license plate number does not match any one of the plurality of registered license plate numbers to a user such as a resident, a towing company, or administrator.

Provided is a ultra-high-sensitivity assay in which the assay can be made on a commonly used assay apparatus such as an absorptiometer and a plate reader or with naked eyes. The high-sensitivity assay in which the assay can be made on a commonly used assay apparatus or with naked eyes can be provided by combining an enzyme cycling method using thio-NAD(P) as a coenzyme, a labeling enzyme and a substrate for the labeling enzyme optimally, and by amplifying thio-NAD(P)H, which is a signaling substance, exponentially and then quantifying the thio-NAD(P)H colorimetrically.

Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers.

A primary set of sensors is situated along a highway approaching a weighing / inspection station, and a secondary set of sensors is situated along a ramp that is traversed by vehicles exiting the highway into a weighing / inspection station. The secondary sensors use vehicle identification based upon transponders and / or alternate means (such as license plate readers) that are suitable for lower speeds and for vehicles not equipped with transponders. The sensors are coupled to a processing system that accesses a database storing data correlated to vehicles using the highway. Upon the sensing of certain parameters by the primary sensors, the vehicles are directed to exit the highway to the weigh station entry ramp. The secondary set of sensors along the ramp provide an ability to identify transponder and non-transponder equipped vehicles and more accurate sensing capability due, among other things, to the slower speed of the vehicle while passing sensors.

The invention provides a portable apparatus for reading a license plate, sensing speed and recognizing a face, which comprises a camera placed on the front right and front left and both sides of the system which enables the system to capture images and recognize faces, LED lighting located around the cameras which are directed towards a visual direction and which enable capturing of legible images even at night time, police lights placed at the front and rear faces of the base which can flash on and off when necessary, an alarm control device, Ethernet connection forming the network, cooling device which eliminates the heat formed inside the apparatus, a control card which operates the police lights, modem which continuously provide wireless communication and a top cover which encloses the entire system.

A micro-plate reader for use with a portable electronic device having a camera includes an opto-mechanical attachment configured to attach / detach to the portable electronic device and includes an array of illumination sources. A slot in the opto-mechanical attachment is dimensioned to receive an optically transparent plate containing an array of wells. Optical fibers are located in the opto-mechanical attachment and transmit light from each well to a reduced size header having, wherein the fiber array in the header has a cross-sectional area that is ≤10× the cross-sectional area of the wells in the plate. A lens located in the opto-mechanical attachment transmits light from the header fibers to the camera. Software executed on the portable electronic device or other computer is used to process the images to generate qualitative clinical determinations and / or quantitative index values for the separate wells.

A license plate reader uses a neural network to determine a plurality of predicted license plate regions within an image. The plurality of predicted license plate regions is transferred to an optical character recognition unit that performs optical character recognition on the plurality of predicted license plate regions to output a plurality of predicted character sequences. The license plate reader receives the output of the optical character recognition unit that contains the plurality of predicted character sequences and analyzes the plurality of predicted character sequences to determine a best estimate for a character sequence in the image.

Methods for concurrently processing multiple biological chip assays by providing a biological chip plate comprising a plurality of test wells, each test well having a biological chip having a molecular probe array; introducing samples into the test wells; subjecting the biological chip plate to manipulation by a fluid handling device that automatically performs steps to carry out reactions between target molecules in the samples and probes; and subjecting the biological chip plate to a biological chip plate reader that interrogates the probe arrays to detect any reactions between target molecules and probes.

A method and system is provided for improving and organizing workflow of a computed radiography system comprising of one or more storage phosphor readers, and multiple workstations which control and receive image data from the readers. In one embodiment, the method comprises temporarily associating an imaging cassette, containing a storage phosphor imaging plate, with a specific workstation. The imaging cassette is transported to the reader and inserted into the reader. The image plate reader is temporarily associated with the specific workstation. This temporary association allows the workstation to control the reader; and allow for sending of an X-ray image data from the reader only to the specific workstation. In other embodiments, this workflow may be adapted for use with networks having a plurality of image plate readers and a plurality of workstations.

A wafer alignment system is provided for performing a unidirectional scan-exposure. The wafer alignment system includes a plurality of wafer stages successively moving from a first position to a second position of a base cyclically. The wafer alignment method also includes an encoder plate having a first opening and a second opening. Further, the wafer alignment system includes a plurality of encoder plate readers and a plurality of wafer stage fiducials on the wafer stages. Further, the wafer alignment system also includes an alignment detection unit above the first opening of the encoder plate.

The invention provides a portable apparatus for reading a license plate, sensing speed and recognizing a face, which comprises a camera placed on the front right and front left and both sides of the system which enables the system to capture images and recognize faces, LED lighting located around the cameras which are directed towards a visual direction and which enable capturing of legible images even at night time, police lights placed at the front and rear faces of the base which can flash on and off when necessary, an alarm control device, Ethernet connection forming the network, cooling device which eliminates the heat formed inside the apparatus, a control card which operates the police lights, modem which continuously provide wireless communication and a top cover which encloses the entire system.

This invention provides a high throughput survival assay, using uptake of a marker dye (e.g. a fluorescent dye) as a marker of death of a nematode. The assay permits high throughput screening of thousands of compounds possible. By the application of automated worm handling technology we are able to accurately dispense nematodes into 384 well microtitre plates, at rates many thousand of Limes faster than previously possible. In addition, we have automated the analysis of survival by the use of a fluorometric plate reader that quantitates the degree of fluorescence within each well.

The present invention is directed to a rapid, quantitative screening procedure of γ-aminobutyric acid (GABA)-mediated halide transport in cells with the use of a conventional 384-well fluorescence plate reader (FLIPR). The halide sensor is a novel yellow fluorescent protein (YFP) with mutations at positions 46 / 148 / 152 that exhibits bright fluorescence at 37° C.

The invention relates to a fluorescent staining kit for rapid detection on biological cell viability, and application of the same. The kit comprises an anthocyanin dye and a propidium iodide dye. The detection comprises the following steps of: adding the anthocyanin dye and the propidium iodide in the kit are added in cell suspension, observing and counting the cells by a fluorescence microscope or detecting the cells by a microporous fluorescent plate reader under wavelength of emitted light, and reflecting the viability of the cells in biologic sample liquid according to the strength of fluorescence values of different colors. The kit can be used for rapidly observing or detecting the amount of living cells and the amount of dead cells of various cells in the sample in real time, is visual and handy, eliminates the inference of other substances and the defect of long culture cycle, and is not only suitable for animal cells, but also suitable for most gram negative bacteria and gram positive bacteria.

A device and system that allows an inverted microscope with a movable x-y stage to also function as a time-resolved plate reader. The device, which is sized to fit into the condenser holder of an inverted microscope, not only provides plate-reading function at very low cost compared to that of purchasing a plate reader, but it also allows the correlation of plate reader measurements with images of a specimen. The device and system is also able to measure other parameters associated with cell metabolism, such as pH changes.

Disclosed are devices or apparatus and methods for membrane-, bead- or cell-based biomolecule analysis, where a detectable signal can be measured after affinity binding and / or enzymatic reaction occurs on the membranes, beads or cells. The apparatus or device can rapidly separate the beads or cells from the liquid solution without using centrifugation, vacuum, or magnetic force. The apparatus or device includes a sample well for receiving a sample containing cells or beads, as well as other aqueous solutions, a porous filter membrane at the bottom of the well which is capable of retaining the cells or beads on its upper surface by size exclusion, and an absorbing plug in touch with the filter membrane for removing the liquid solution. Multiple devices can be arranged in a multi-well array in a plate so that signals from multiple assays can be directly analyzed in a plate reader or an imaging instrument.

The invention relates to a gold nanoparticle growth based method for detection of glucose. The steps include: (1) adding glucose oxidase and a glucose solution with a concentration of 0-1.0mM into a phosphate buffer solution respectively, conducting incubation at room temperature for 10min to obtain an incubation solution; and 2) adding 5nm gold nanoparticles and chloroauric acid into the incubation solution, carrying out room temperature incubation for 30min, taking photos, and using a Synergy 2 micropore plate reader to read the absorbance value at the place of 530nm correspondingly. The method employs glucose oxidase to catalyze production of gluconic acid from glucose, generates hydrogen peroxide and reduces chloroauric acid into elemental gold, promotes the growth of 5nm gold nanoparticles, also causes color change so as to conduct glucose content determination. The whole detection process only needs one enzyme, i.e. glucose oxidase, has no need of expensive instrument, not only reduces the cost, but also is fast, convenient and is easy to implement.

The invention provides methods and systems for identifying objects transitioning boundaries defined around geographic areas using sensors. Objects that are vehicles are detected by license plate reader sensors. Boundaries defining geographic zones and sub-zones are pre-defined graphically by reference to a map or computed based on locations of sensors located within the geographic region. When sensor coverage is incomplete, soft zones are computed and defined. Soft zones are computed using probabilistic models, and are developed to minimize the number of escape paths from any sub-zone around sensor locations. Using these probabilistic models, additional sensors can be optimally placed for further enhancement of the detection system.

Molecular beacon for detecting an infection and / or expression or a mutation of a disease marker for diagnostics and pharmacogenomics. The molecular beacon is capable of hybridizing a disease-related RNA or DNA of a disease marker in a specimen obtained from a living subject, thereby emitting a signal detectable without a need for signal amplification. The disease marker includes a genetic sequence specific to a pathogen including a flu virus, a cancer cell marker, and a drug resistance-related genetic mutation marker for a drug resistant cancer and infectious pathogen. To detect a disease cell, a specimen containing one or more cells is obtained from a living subject, and fixed by an organic solvent. A molecular beacon is then added to the specimen, followed by staining nuclei of the cells in the specimen. The signal is detectable with a microscope, FACS scan, ELISA plate reader, Scanner, or any combinations thereof.

The invention discloses a parking system based on a two-dimensional code. The parking system of the two-dimensional code comprises a software part and a hardware part. The software part comprises recognition software used for vehicle management, analysis and processing software used for data analysis, navigation software, a two-dimensional code generation module used for generating a two-dimensional code image, and a two-dimensional code recognition module used for recognizing the two-dimensional code image. The hardware part comprises a weighing sensor, a vehicle license plate reader which is used for recognizing a vehicle license plate corresponding to an indoor parking space, an outdoor display and a two-dimensional code reader. According to the invention, the information code is uniquified through the navigation software, the recognition software and the data analysis and processing software, and the two-dimensional code including the parking space information, a path and the like is generated; each area parking space code is unique and is very clear; reverse query of the parking space information is easily realized; information indication and guidance service of a parking lot are realized through additional facilities; the utilization of the parking lot can be improved; and the parking system is convenient for a user.

A road traffic congestion degree estimation system includes smart plate readers that detect vehicles driven on a general road externally leading to a resort. The numbers of local vehicles and strange vehicles that currently exist in the resort are calculated, based on the number of vehicles approaching the resort, the number of vehicles receding from the resort, and information of smart plates of the detected vehicles. Furthermore, a prospective degree of traffic congestion on an expressway, which introduces the strange vehicles receding from the resort via the general road into areas where the strange vehicles are based, is estimated based on the above calculated numbers of local vehicles and strange vehicles. In this estimation, the number of strange vehicles contributes more greatly to an increase in the degree of traffic congestion than the number of local vehicles does.

A fluorescence microtitre plate reader comprises a plate reader body (2) having a base (3), top (5) and sidewalls (4), the top of the plate reader comprising at least one seat (6) dimensioned for receipt of a microtitre plate (7), the at least one seat defining an aperture (8) in the top of the plate that substantially corresponds to a base of the plate. The plate reader also includes means for illuminating wells of a microtitre plate with light from beneath the plate having an excitation wavelength, and a camera (10) and lens (11) assembly for capturing light emitted by the wells of the microtitre plate and generating a digital image, in which regions of light intensity of the image correspond to wells of the microtitre plate. An optical system is disposed within the plate reader body for expanding the distance to image plane of light, the optical system comprising a first mirror (15) disposed to receive light from the wells of the microtitre plate and reflect the light to second mirror (16) which is disposed to reflect incident light to the camera lens. The means for illumining the wells of the microtitre plate comprises a ring light (12) that embraces the camera and lens assembly.

Molecular beacon for detecting an infection and / or expression or a mutation of a disease marker for diagnostics and pharmacogenomics. The molecular beacon is capable of hybridizing a disease-related RNA or DNA of a disease marker in a specimen obtained from a living subject, thereby emitting a signal detectable without a need for signal amplification. The disease marker includes a genetic sequence specific to a pathogen including a flu virus, a cancer cell marker, and a drug resistance-related genetic mutation marker for a drug resistant cancer and infectious pathogen. To detect a disease cell, a specimen containing one or more cells is obtained from a living subject, and fixed by an organic solvent. A molecular beacon is then added to the specimen, followed by staining nuclei of the cells in the specimen. The signal is detectable with a microscope, FACS scan, ELISA plate reader, Scanner, or any combinations thereof.

The invention discloses an important vehicle pass system based on an intelligent license plate. The important vehicle pass system comprises an intelligent license plate system, a backlist database, an analysis alarming system, and a monitoring system. The intelligent license plate system comprises an intelligent license plate, an intelligent license plate reader, and a control center. The control center comprises a database, and the intelligent license plate is disposed on the eye-catching position of the important vehicle. The intelligent license plate comprises the pass information of the important vehicle, and the intelligent license plate reader is disposed on the traffic intersection or the check point, and is used to identify the intelligent license plate on the important vehicle entering the traffic intersection or the check point, and is used to transmit the intelligent license plate information back to the control center. After comparing the intelligent license plate information with the information of the database, the control center is used to determine whether the vehicle can pass. The important vehicle pass system has advantages of high intelligentization degree, no artificial intervention, high efficiency, and ability of realizing free stream.