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Gold nanoparticle growth based method for detection of glucose

A technology of gold nanoparticles and glucose, which is applied in the direction of analyzing materials through chemical reactions and observing the influence of chemical indicators on materials, etc., which can solve the problem of expensive peroxidase, high cost, and environmental influence on activity Large and other problems, to achieve the effect of easy implementation and lower detection cost

Active Publication Date: 2015-10-21
天津南开大学资产经营有限责任公司
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Problems solved by technology

A disadvantage of this method is that peroxidase is very expensive compared with glucose oxidase, and its activity is greatly affected by the environment, which is inconvenient and expensive to use

Method used

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  • Gold nanoparticle growth based method for detection of glucose
  • Gold nanoparticle growth based method for detection of glucose
  • Gold nanoparticle growth based method for detection of glucose

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Embodiment

[0031] A method for detecting glucose based on gold nano growth, comprising the steps of:

[0032] 1) In a phosphate buffer solution with a concentration of 0.01M and a pH of 7.4, mix a group of glucose solutions of a specific concentration with glucose oxidase (GOx) respectively, and incubate at room temperature for 10 minutes to obtain a group of incubation solution a, a specific concentration of The concentration of glucose solution is 0, 0.1, 0.3, 0.5, 0.7, 0.9, 1.0mM respectively;

[0033] 2) Add 5nm gold nanoparticles and chloroauric acid to the above incubation solutions respectively, the concentration of 5nm gold nanoparticles in the incubation solution b is 8.3nM, the concentration of chloroauric acid is 0.16mM, and incubate at room temperature for 30min to obtain a set of For incubation solution b, take photos of incubation solution b and read their corresponding absorbance values ​​at 530nm with a Synergy 2 microplate reader, and fit the standard curve according to ...

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Abstract

The invention relates to a gold nanoparticle growth based method for detection of glucose. The steps include: (1) adding glucose oxidase and a glucose solution with a concentration of 0-1.0mM into a phosphate buffer solution respectively, conducting incubation at room temperature for 10min to obtain an incubation solution; and 2) adding 5nm gold nanoparticles and chloroauric acid into the incubation solution, carrying out room temperature incubation for 30min, taking photos, and using a Synergy 2 micropore plate reader to read the absorbance value at the place of 530nm correspondingly. The method employs glucose oxidase to catalyze production of gluconic acid from glucose, generates hydrogen peroxide and reduces chloroauric acid into elemental gold, promotes the growth of 5nm gold nanoparticles, also causes color change so as to conduct glucose content determination. The whole detection process only needs one enzyme, i.e. glucose oxidase, has no need of expensive instrument, not only reduces the cost, but also is fast, convenient and is easy to implement.

Description

technical field [0001] The invention relates to a method for quantitatively detecting glucose content, in particular to a method for detecting glucose based on the growth of gold nanometers. Background technique [0002] With the improvement of people's living standards, the change of diet structure and the reduction of physical labor, the incidence of diabetes is increasing day by day. At present, there are more than 200 million diabetic patients in the world, which has become one of the major diseases that threaten human health. In addition, diabetes can also cause a series of complications, such as high blood pressure, renal failure, blindness, stroke, heart disease, etc. Therefore, The diagnosis and treatment of diabetes has always been a major issue facing the world medicine. Blood sugar concentration is an important clinical indicator of diabetes. Early diagnosis of diabetes can be realized by detecting blood sugar. In order to better control this disease, diabetic pa...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 刘定斌熊艳梅
Owner 天津南开大学资产经营有限责任公司
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