43 results about "Luminescent Assays" patented technology

An apparatus for performing a binding assay for an analyte of interest in a sample based upon measurement of clectrochemiluminescence at an electrode surface comprising: (a) a cell defining a sample; (b) an electrode adjacent a portion of the sample containing volume; (c) a voltage control device for impressing electrochemical energy upon the electrode sufficient to generate luminescence; (d) means for magnetically collecting particles along the electrode surface; and (e) a light detection device for measuring the luminescence.

The present invention provides stabilized chemiluminescent formulations for use in in vitro diagnostics, including competitive as well as sandwich-type immunological assays. The stabilized assay system may be composed of two components, where the first component may contain a chemiluminescent organic compound, an enhancer, a homogenizing agent, and a suitable buffer with formulations having a pH range from about 7.2 to about 12, and optionally a solubilizing agent. The chemiluminescent system of the present invention is useful in immunoenzymatic analytical procedures, such as immunometric, competitive binding and sandwich type assays. In such immunoassays employing the chemiluminescent system of the present invention, the detectable light signal shows a proportional decay with time in the test samples and standards, so that the decay of the light emitted does not effect the concentration of the analyte measured over the entire analyte measurement range of the immunoassay. This allows accurate measurement of analyte concentrations in a test sample over extended periods of time.

Assay kits for increasing the sensitivity of luminescent assays are provided that include an organic compound that reduces luminescence that is not dependent on the presence of an analyte by at least about 10 fold, and that reduces, maintains, or increases the luminescence that is dependent on the presence of an analyte.

A method to detect the presence or amount of at least one molecule for an enzyme-mediated reaction in a multiplex luminogenic / nonluminogenic assay is provided.

The present invention provides protein fragment complementation assays for drug discovery, in particular to identify compounds that activate or inhibit cellular pathways. Based on the selection of an interacting protein pair combined with an appropriate PCA reporter, the assays may be run in high-throughput or high-content mode and may be used in automated screening of libraries of compounds. The interacting pair may be selected by cDNA library screening; by gene-by-gene interaction mapping; or by prior knowledge of a pathway. Fluorescent and luminescent assays can be constructed using the methods provided herein. The selection of suitable PCA reporters for high-throughput or high-content (high-context) assay formats is described for a diversity of reporters, with particular detail provided for examples of monomeric enzymes and fluorescent proteins. Methods are described for constructing such assays for one or more steps in a biochemical pathway; testing the effects of compounds from combinatorial, natural product, peptide, antibody, nucleic acid or other diverse libraries on the protein or pathway(s) of interest; and using the results of the screening to identify specific compounds that activate or inhibit the protein or pathway(s) of interest. Single-color and multi-color assays are disclosed. Further disclosed are universal expression vectors with cassettes that allow the rapid construction of assays for a large and diverse number of gene / reporter combinations. The development of such assays is shown to be straightforward, providing for a broad, flexible and biologically relevant platform for drug discovery.

What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and / or the assay-performance-substance; incubating the composition to form a complex which includes a particle and the labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.

A chemiluminescence immunoassay method for detecting zearalenone toxin in the technical field of biological detection engineering comprises the following steps of: linking ZEN semiantigen with OVA to obtain ZEN-OVA; extracting a sample to be measured to obtain an extract; coating ZEN-OVA on a 96-well white plate at the optimum coating concentration, followed by sealing, respectively adding a known solution of the ZEN pure product and anti-zearalenone monoclonal antibody and a mixed solution of the extract of the sample to be measured and anti-zearalenone monoclonal antibody at different concentrations, followed by incubation; adding HRP-labeled secondary antibody after incubation, and adding a luminescence substrate for luminescent determination to obtain a result; making a standard curve; and acquiring the ZEN concentration in the extract of the sample to be measured through the standard curve. The method provided by the invention can be used to rapidly detect the amount of ZEN in the sample to be measured; in the meanwhile, the method has strong pertinency of detection object and results in high accuracy.

The invention provides chemiluminescent assays that incorporate a film including at least one chemiluminescent precursor immobilized therewith which produces a triggerable chemiluminescent compound, the film being free of compounds which generate singlet oxygen and being adapted for use with a sensitizer-labeled agent or agent probative of the analyte.

The invention provides chemiluminescent assays that incorporate a film including at least one chemiluminescent precursor immobilized therewith which produces a triggerable chemiluminescent compound, the film being free of compounds which generate singlet oxygen and being adapted for use with a sensitizer-labeled agent or agent probative of the analyte.

We describe a detection module used in an apparatus (and a system of the apparatus and the detection module) for conducting luminescence assays in multi-well plates, wherein the detection module includes a housing comprising a light detector and a control board. The apparatus and detection module each include engagement elements that align and engage the detection module with the apparatus in the system.

Provided is a technique capable of easily performing a microbial test with high accuracy. A microbial test kit of the present disclosure includes: a first syringe capable of collecting a specimen and having a first syringe needle; a second syringe containing an ATP extraction reagent and having a second syringe needle; a sealed reaction container having a first opening, a first sealing member fitted to the first opening, a nozzle, a nozzle cap covering the nozzle, and a filter disposed so as to partition the first opening and the nozzle; a waste liquid container including a second opening and a second sealing member fitted to the second opening, and sealed under reduced pressure; and a luminescence measurement container that includes a third opening and a third sealing member fitted to the third opening, stores a luminescent reagent that emits light in presence of ATP, and is sealed under reduced pressure.

The invention relates to a stress luminescence measurement device and a stress luminescence measurement method. The stress luminescence measurement device according to a first aspect is provided with a load application mechanism configured to deform a sample (1) by applying a load to the sample (1), a light source (31) configured to emit excitation light to a stress luminescent object (2) arranged on a surface of the sample (1), a camera (40) configured to image luminescence of the stress luminescent object (2), and a controller (50) configured to control the load application mechanism, the light source (31), and the camera (40). The controller (50) acquires a deformation state of the sample (1) at the imaging timing by the camera (40) and stores the acquired deformation state of the sample (1) in association with the image captured by the camera (40) in a memory (502).

The invention belongs to the technical field of chemiluminescence measurement, and provides a full-automatic chemiluminescence measuring instrument. An incubating component is installed on the base of a transmission rack, and a shell breaking sampling mechanism, a magnetic separation mechanism and a testing mechanism are arranged above the base. , and set a plurality of tip slots for placing multiple tips on the incubation component, so that the incubation component is kept at a constant temperature and the limit is equipped with multiple reagent strips with multiple storage pools. The multiple storage pools seal different samples, and After the shell breaking sampling mechanism is plugged and removed, the gun head is controlled to puncture the capsule of the reagent strip and sampled, and the gun head is controlled to take samples to obtain magnetic beads and solutions for the magnetic separation mechanism to separate and leave the magnetic beads to put into multiple containers. The test pool in the object pool, and then the test mechanism is socketed with the test pool to form a light-shielding environment to read the light intensity of the solution to be tested in the test pool, so that it can automatically break the shell of multiple reagent strips and pick and place multiple tips. , Sampling, magnetic separation and testing operations, not only simple in structure, but also high in efficiency and low in cost.

The invention relates to a nanometer magnetic particle chemiluminescence detection kit for triiodothyronine as well as a preparation method and a detecting method of the nanometer magnetic particle chemiluminescence detection kit. The kit comprises a first reagent, a second reagent and a magnetic separating agent, wherein the first reagent is a solution containing a fluorescein labeled triiodothyronine antibody; the second agent is a solution containing an alkaline phosphatase labeled triiodothyronine antigen; and the magnetic separating agent is a suspension containing magnetic particles coated by a fluorescein antibody. According to the invention, the triiodothyronine can be quantitatively detected with lower cost, higher accuracy and higher precision.