Chemiluminescence assay kit for detecting C-peptide content in serum and detection method

Abstract

The invention belongs to the field of medical detection and discloses a chemiluminescence assay kit for detecting C-peptide content in serum and a detection method. The assay kit comprises: an M reagent, an R reagent, a C-P calibrator, a pH adjuster, a reaction enhancer, and a chemiluminescent substrate. The M reagent contains a magnetic particle coated with a C peptide-biotinylated antibody at aconcentration of 2[mu]g / mL, and a Tris buffer solution at a concentration of 0.2mol / L. The R reagent contains a C-P-AP labeled antibody at a concentration of 1[mu]g / mL, and a Tris buffer solution at aconcentration of 0.1mol / L. The C-P calibrator comprises two different concentrations of C-peptide samples and a 0.1mol / L concentration of Tris buffer solution. The assay kit for detecting C-peptide has advantages of strong specificity and good stability, thereby improving the sensitivity and specificity of the detection; has a good stability in the whole system; is simple in operation; and is suitable for use with a clinical full automatic chemiluminescence assay instrument.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a chemiluminescence assay kit and a detection method for C-peptide content in serum. Background technique [0002] Human C-peptide (C-P) is proinsulin produced simultaneously with insulin through enzymatic cleavage in pancreatic beta cells, and has no biological activity, but it is necessary to ensure the normal structure of insulin. Although C-peptide and insulin are produced isomolecularly, C-peptide concentrations are 5-10 times higher than insulin after fasting due to the longer half-life of C-peptide. C-peptide is mainly degraded in the kidney, and partially excreted in the urine in the original form. Since the metabolism of C-peptide in the liver does not exceed 10%, the concentration of C-peptide can better reflect the function of pancreatic β-cells compared with the concentration of insulin in peripheral blood. At present, the laboratory detection methods of C...

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Problems solved by technology

[0004] Aiming at the shortcomings of unsatisfactory sensitivity and stability in the detection of C-peptide in the prior art, the present invention provides a C-peptide detection method with high sensitivity, good stability, strong specificity, wide dynamic range and suitable for automatic operation. Chemiluminescence determination kit and detection method for C-peptide content in serum

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