The invention discloses identification and an application of a tomato SolyWRKY54
transcription factor in regulating and controlling
tomato yellow leaf curl virus. According to the invention, a
WRKY transcription factor gene SolyWRKY54, which responds to the
tomato yellow leaf curl virus, is identified from tomatoes;
protein coded by the
gene belongs to III sub-family in a WRKY transcription factorfamily. A semiquantitative PCR result shows that a susceptible variety, namely 'Jinpeng No.1', is higher than a
disease-resistant variety, namely 'Zheza 301', in
virus accumulation. Based upon fluorescent
quantitative PCR analysis, it is indicated that the expression amount of the SolyWRKY54
gene is reduced in the
disease-resistant tomato variety, namely 'Zheza 301'. Through a
virus-induced genesilencing experiment, it further provides a negative regulation function of the SolyWRKY54 gene in a process of responding to the
tomato yellow leaf curl virus. The SolyWRKY54
transcription factor gene, which is identified by the invention, is beneficial to analyzing and researching a resistance mechanism of tomatoes responding to the tomato yellow
leaf curl virus; and the SolyWRKY41 transcriptionfactor is applicable to tobacco
disease-resistance breeding.