Identification and application of tomato SolyWRKY54 transcription factor in regulating and controlling tomato yellow leaf curl virus

Abstract

The invention discloses identification and an application of a tomato SolyWRKY54 transcription factor in regulating and controlling tomato yellow leaf curl virus. According to the invention, a WRKY transcription factor gene SolyWRKY54, which responds to the tomato yellow leaf curl virus, is identified from tomatoes; protein coded by the gene belongs to III sub-family in a WRKY transcription factorfamily. A semiquantitative PCR result shows that a susceptible variety, namely 'Jinpeng No.1', is higher than a disease-resistant variety, namely 'Zheza 301', in virus accumulation. Based upon fluorescent quantitative PCR analysis, it is indicated that the expression amount of the SolyWRKY54 gene is reduced in the disease-resistant tomato variety, namely 'Zheza 301'. Through a virus-induced genesilencing experiment, it further provides a negative regulation function of the SolyWRKY54 gene in a process of responding to the tomato yellow leaf curl virus. The SolyWRKY54 transcription factor gene, which is identified by the invention, is beneficial to analyzing and researching a resistance mechanism of tomatoes responding to the tomato yellow leaf curl virus; and the SolyWRKY41 transcriptionfactor is applicable to tobacco disease-resistance breeding.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering and relates to the functional research and application of a WRKY III transcription factor SolyWRKY54 in tomato. The pattern of SolyWRKY54 gene responding to tomato yellow leaf curl virus in different tomato varieties provides a basis for further research on the response of WRKY transcription factors to tomato yellow leaf curl virus. Background technique [0002] Tomato (Solanum lycopersicum L.), also known as tomato, originated in western South America and is an annual herb. Tomatoes are widely used in various scientific researches, such as breeding, fruit development and ripening, stress resistance regulation, etc. (Cuartero et al., J Exp Bot 2006, 57: 1045-1058; Kennedy et al., Annu RevEntomology 2003, 48: 51-72; Klee et al., Annu Rev Genet 2011, 45:41-59; Schauer et al., Plant Cell 2008, 20:509-523). [0003] Tomato yellow leafcurly virus (TYLCV) is a single-stranded circular DNA viru...

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Problems solved by technology

The symptoms of plant infection with TYLCV are obvious, which will cause yellowing, shrinkage, and wrinkling of the entire leaf, and prolong the ripening period of the fruit, seriously affecting the yield and quality (Glick et al., Plant Protect Sci2009, 45: 81-97)

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