Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

WRKY transcription factor for regulating and controlling aging of leaf blades and multiple stress tolerance and application thereof

A technology of transcription factors and coding genes, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., and can solve problems such as ignorance of genetic and molecular mechanisms of retention and regulation

Inactive Publication Date: 2013-03-13
FUDAN UNIV
View PDF2 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are few studies on AtWRKY6, which mainly stay at the phenomenon observation stage; there are also few studies on the regulation of plant leaf senescence and multiple stress resistance by the WRKY family, especially the genetic and molecular mechanisms of its regulation are still almost unknown

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • WRKY transcription factor for regulating and controlling aging of leaf blades and multiple stress tolerance and application thereof
  • WRKY transcription factor for regulating and controlling aging of leaf blades and multiple stress tolerance and application thereof
  • WRKY transcription factor for regulating and controlling aging of leaf blades and multiple stress tolerance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Acquisition of Arabidopsis transcription factor AtWRKY6 insertion mutant material.

[0023] 1.1 Arabidopsis material preparation

[0024] Leaves of Arabidopsis Col-0 cultured at 23°C for 30 days under 16h L / 8h D light were taken.

[0025] 1.2 RNA extraction

[0026] Take about 0.1 g of Arabidopsis material. After the liquid nitrogen is fully ground, transfer to a 1.5ml centrifuge tube, add 1ml TRIzol (Invitrogen Company), mix well, let stand at room temperature for 15 minutes, add 0.2ml chloroform:isoamyl alcohol (24:1), shake vigorously for 15 seconds, and then room temperature Leave for 5 minutes, centrifuge at 13000rpm, 4°C for 15 minutes. Take the supernatant and add an equal volume of isopropanol, mix carefully, place at room temperature for 15 minutes, centrifuge at 13000 rpm, 4°C for 15 minutes. The precipitate was washed with 70% ethanol and dried at room temperature for 15 minutes. Dissolve in an appropriate amount of ddH treated with 0.1% DEPC ...

Embodiment 2

[0031] Example 2: Functional analysis of Arabidopsis transcription factor AtWRKY6.

[0032] 2.1 Sequence comparison analysis

[0033] The homology between AtWRKY6 and other Arabidopsis WRKY family proteins was analyzed by Genedoc software. The analysis results showed that AtWRKY6 had a typical conserved WRKY domain ( figure 1 ). The phylogenetic tree of AtWRKY6 and other Arabidopsis WRKY family proteins was established using MEGA software. The results showed that AtWRKY6 belonged to the WRKY-IIa subfamily and had the closest relationship with AtWRKY31, AtWRKY42 and AtWRKY47. There are also proteins with high homology to AtWRKY6 in plants such as rice, corn, rapeseed, and soybean. figure 2 Show the phylogenetic relationship of WRKY6 proteins in plants.

[0034] 2.2. Analysis of leaf senescence indicators of mutants

[0035] 2.2.1 Substrate culture of Arabidopsis thaliana:

[0036] Matrix components: vermiculite: black soil: perlite 9 : 3 : 0.5

[0037] Nutrient solutio...

Embodiment 3

[0041] Example 3: Application of transcription factor WRKY6

[0042] of the present invention WRKY6 The gene can be constructed into the existing plant expression vector by existing methods, and can be added before its transcription start nucleotide including constitutive promoter, enhanced promoter, inducible promoter, tissue-specific promoter, developmental Any promoter, including stage-specific promoters. For easy transfer WRKY6 Genetic plant cells or plants are identified and screened, and the vectors used can be processed, such as adding plant selectable markers ( BAR Gene, GUS gene, luciferase gene, etc.) or antibiotic markers for resistance (kanamycin, streptomycin, hygromycin, etc.). The transformed plant host can be either a monocot or a dicotyledon, such as rice, corn, soybean, poplar, lawn grass and the like. carrying the present invention WRKY6 The expression vector of the gene can transform plant cells or tissues by conventional biological methods such ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of plant gene engineering and specifically relates to a WRKY transcription factor for regulating and controlling aging of leaf blades and multiple stress tolerance and an application thereof. A plant WRKY6 protein provided by the invention is derived from arabidopsis, rape and other species, but is not limited to the species, and the plant WRKY6 protein comprises an analogous protein, wherein the homeology of the analogous protein with AtWRKY6 (the amino acid sequence is as shown in SEQ ID NO: 2) in the arabidopsis is higher than 30%. An encoding gene of the arabidopsis WRKY6 protein is one of the following nucleotide sequences: (1) SEQ ID NO: 1 in a sequence table; and (2) polynucleotide with the amino acid sequence as shown in the SEQ ID NO: 2 in a coded sequence table. The invention can provide a protein resource and a method in the aspects of deferring the aging of the leaf blades and improving the yield, quality and multiple stress tolerance of crops.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to a WRKY transcription factor for regulating leaf senescence and multiple stress resistance and its application. Background technique [0002] Plant senescence is a process of a series of programmed gradual declines in individual plants, eventually leading to death. Leaf senescence is an integral part of plant growth and development, which constitutes the last stage of plant leaf development. It is considered to be a type of programmed cell death (PCD), which proceeds in a leaf-age-dependent manner and is induced by many environmental factors. Interaction of complex internal and external factors during growth and development. Therefore, leaf senescence is not a passive process, but an actively regulated process. During leaf senescence, cell structure, physiological metabolism, and gene expression undergo a series of orderly changes. In addition, due ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10A01H5/00
Inventor 蒯本科高炯朱政梁宁菁
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com