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Tomato-resistant meloidogyne incognita related gene and application thereof

A technology of root-knot nematode and genes, applied in the field of molecular biology and biology, which can solve the problems of just starting and lagging research on tomato WRKY transcription factors

Inactive Publication Date: 2019-09-13
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The completion of tomato genome sequencing has laid a good foundation for in-depth research on WRKY transcription factors. Hang et al. identified 81 WRKY transcription factors from the tomato genome database, but the research on their respective functions and molecular mechanisms has only just started. The reports mainly focus on viruses and fungi
The research on tomato WRKY transcription factors related to nematodes is relatively lagging behind, and only a few genes encoding WRKY transcription factors have been cloned so far

Method used

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  • Tomato-resistant meloidogyne incognita related gene and application thereof
  • Tomato-resistant meloidogyne incognita related gene and application thereof
  • Tomato-resistant meloidogyne incognita related gene and application thereof

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Embodiment Construction

[0024] The present invention will be further described below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0025] The nucleotide sequence SEQ ID No.1 is as follows:

[0026] agctgcaccg ccaccacaac cgccggcgac atcagtttta ccaacttcca accaagaaca 60

[0027] acagatgatg ataataacag taacaataac tctgtatttt gctttgaaga tctggttctc 120

[0028] aaacagagcc tagtattgaa tagaaataaa attagtgatg atttcgaaga attgcatcaa 180

[0029] ctttgcaagc catttattaa tcaaccatcc gaaaaattaa cggtcatttc atcacaatca 240

[0030] ccgtcgcaac agcttactga tacgaaatca attcaaccca acagaacttt atcatgtaat 300

[0031] ttacatgctc aaagtcaaac aatcaaaaga aggaagaacc atttaaagaa agtatgtcaa 360

[0032] gtagctgctg atggtttatc ttctgatatg tggtcttgga gaaaatatgg acaaaaacct 420

[0033] attaaaggtt ccccataaccc aaggggatat tacaaatgta gcacttcaaa aggttgtttg 480

[0034] gcccgaaaac aagtggagcg gaatagatcc gacccgaata tgtttattgt cacttataca 540

[0035] gctgagcaca a...

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Abstract

The invention discloses a tomato-resistant meloidogyne incognita related gene and application thereof. The invention provides a tomato-resistant meloidogyne incognita related gene of which the nucleotide sequence is as shown in SEQ ID NO.1, simultaneously provides protein encoded by the gene, and provides application thereof in enhancing the resistance of plants to meloidogyne incognita. A WRKY transcription factor encoding gene SlWRKY1 is separated from tomatoes, and the gene can be introduced into the tomatoes as a target gene to enhance the resistance of the tomatoes to meloidogyne incognita, thereby improving tomato varieties.

Description

technical field [0001] The invention relates to the fields of molecular biology and biotechnology, in particular to a gene related to tomato resistance to root-knot nematode incognita and its application. Background technique [0002] root-knot nematodes ( Meloidogyne spp.) The tomato root-knot nematode disease caused by tomato ( Solanum lycopersicum ) is one of the important diseases, which generally reduces tomato yield by 10-20%, and severe plots can reach more than 75%. Breeding of disease-resistant varieties is one of the most economical, effective and safe methods to prevent and control the disease. Although the molecular breeding research of tomato disease resistance has made some progress, most of them focus on the excavation and application of a single disease resistance gene, and the most widely used disease resistance gene is currently Mi The limitations of thermal instability and the existence of its resistant races in nature limit its wide application. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/82
CPCC07K14/415C12N15/8285
Inventor 陆秀红黄金玲张禹刘志明黄丽英黄琦
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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