171 results about "Salt Tolerant Plants" patented technology

The invention discloses a saline-alkali soil improving method. The method includes the steps of leveling the terrain of selected saline-alkali soil, laying salt disc elimination harge closed underground pipes, installing a bitter water desalting system to desalt crude water and collected salty water of the saline-alkali soil to provide fresh water for irrigation salt content restraining and irrigation, primarily conducting primary salt elimination on the saline-alkali soil through irrigation salt content restraining, adjusting the soil structure through a saline-alkali soil modifier, providing trace elements required by plants, planting salt-tolerant plants for further reducing salt content, conducting underground salt elimination through the combination of drip irrigation-micro jet devices and the salt-tolerant plants, and executing information maintenance management on the area of the saline-alkali soil. The method integrates biology, physics, chemistry and water conservancy methods, and is small in water resource consumption, rapid in effect, not prone to salt returning and more beneficial to plant growth.

The invention relates a treatment and utilization technology of saline soil resources, in particular to a treatment and utilization method of muddy seashore saline soil. The method comprises the following steps of: establishing a water supply system consisting of a fresh water well, a water pump and a water supply pipe; excavating a salt leaching drainage ditch so as to establish a drainage salt leaching system; applying a cow dung and crop stalk fertilizer and a phosphogypsum soil and fertility improving fertilizer; with a small raised field as unit, irrigating fresh water to wash and leach salt; and planting and maintaining vegetation through cultivating the land by machine, sunning the upturned soil, planting the vegetation and irrigating the vegetation, wherein a planting technology with gradient increasing salt tolerance is adopted in planting the vegetation. According to the invention, for the salinity and structural characteristics of the muddy seashore saline soil, the muddy seashore strongly salined soil in the coastal zone is treated and utilized in combination with engineering measures, physical measures and chemical measures on the basis of salt tolerant plant appraisal; and The treatment and utilization method of the muddy seashore saline soil provided by the invention has the advantages of simplicity in operation, resource conservation, soil treatment and utilization cost of less than 166.21 yuan / m2 and plant survival rate of more than 90%.

The invention discloses a soybean HKT-like protein and the coding gene and application thereof, which belongs to the technical field of biology. The soybean HKT-like protein is named as GmSKC1 protein which has an ammonia sequence shown by the SEQ ID NO.2 in a sequence list. The coding gene of the protein is a DNA sequence shown by the SEQ ID NO.1 of the GmSKC1 gene. The soybean HKT-like protein and the coding gene thereof can be used for cultivating salt tolerant plant species, in particular salt tolerant soybean species. The expression of GmSKC1 is inducted by high salt. Under the stress of 150mM NaCl, GmSKC1 is strongly inducted in the soybean tissues such as root and leaves; and the expression of GmSKC1 in the soybean stem has a basically invariable trend. Compared with the non-transgene tobacco, the tobacco with the excessive expression of GmSKC1 remarkably improves the salt tolerance, thereby demonstrating that the GmSKC1 gene has an important action on improving the salt tolerance of plants.

The invention relates to a method for improving coast salty soil by utilizing cotton straw, belonging to the soil improvement technical field. The invention is characterized in that: soil block to be improved is divided on coast salty soil; salty soil more than 20cm higher than ground water level is removed; cotton straws doped with straws are arranged in sequence, flatted and tied up into straw layers with the thickness of 10cm; the straw layers are spread and covered on the salty soil in the same direction and in parallel, broken stones with the grain diameter of 1-2cm are uniformly and additionally covered on the straw layer, the thickness thereof is 5cm, thus forming an isolating layer; so that two isolating layer are formed; and punning is carried out. Ambient salty soil is uniformly covered on the isolating layer; salt tolerant plants are planted in the saline soil; and straw with the thickness of 3cm is covered on the saline soil. By implementing the invention, soil salt content is obviously reduced; and soil organic matter is improved. Isolating effect is obvious, the cotton straw is isolated and also adds soil nutrient, thus being beneficial to plant growth. Operation is simple and convenient, cost is low, and the method can be popularized and applied in large scale; and the cotton straw is recycled.

The present invention belongs to the field of ecological engineering technology. The biomass of the strand mallow is large, its body interior contains a great quantity of biomass energy. Said invention is characterized by adopting various measures to develop and utilize biomass energy of every portion of strand mallow and converte it into salt-tolerant plant with higher economic benefits.

The present invention discloses a protein related to salt tolerance, a coding gene thereof and an application thereof. The protein provided refers to the following protein (a) or the protein (b). The protein (a) consists of amino acid sequences shown in sequence 1 of a sequence table. The protein (b) related to plant salt tolerance is derived from the sequence 1 in which the amino acid sequences of the sequence 1 are replaced by one or several amino acid residues, and / or deleted, and / or added. The present invention also provides a coding gene of the protein. A transgenic plant of improved salt tolerance can be obtained by leading the coding gene of the protein into the plant, and novel plants of improved salt tolerance can be obtained by grafting the transgenic plant to other plants. The present invention has great significance in the cultivation of salt tolerant plants.

The invention provides a high-efficiency, energy-saving and emission-reduction land-based sea water circulation culture method based on an ecosystem level. According to the method, an integral sea water circulating system isolated from the ocean is established on the land and is divided into a high-order culture region, a filter-feeding culture region, a salt-tolerant plant wetland and an ecological purification region; sea water is injected into the integral sea water circulating system, then sequentially flows through the high-order culture region, the filter-feeding culture region, the salt-tolerant plant wetland and the ecological purification region and finally returns to the high-order culture region; the high-order culture region is used for carrying out culture production operation on energy-input-type ocean economical organisms; the filter-feeding culture region is used for carrying out microalgae culture operation and carrying out culture production operation on ocean economical organisms capable of filter-feeding the microalgae; the salt-tolerant plant wetland is used for carrying out planting operation on salt-tolerant plants capable of absorbing nutritive salts; and the ecological purification region is used for carrying out water quality ecological purification operation.

The invention discloses a fiber sticking strongly saline-alkali soil engineering improvement and ecological establishment method. A concealed conduit salt elimination system is laid underground fibersticking strongly saline-alkali soil, an open ditch drainage system is dug according to the pipeline running layout of the concealed conduit salt elimination system, a fiber sticking layer is broken by deep scarification, gypsum is applied, irrigation salt-leaching is performed after rotary tillage blending, suitable salt-tolerant plant types are determined according to irrigation water quality, and plants are planted by ridging. Concealed conduit drainage is combined with open ditch salt elimination, underground water level is controlled, soil salt elimination is accelerated, soil is improvedby the gypsum, salt-leaching efficiency is improved, and rapid engineering improvement of the fiber sticking strongly saline-alkali soil is realized.

The invention provides a method and a system for desalting soil of coastal saline and alkaline land. The method for desalting the soil of the coastal saline and alkaline land includes the following steps of performing soil excavating and blocking up to the coastal saline and alkaline land to be desalted to form a platform field and bottom land; constructing a microalgae cultivating device in the bottom land and planting salt-tolerant plants on the platform field; subjecting the platform field to salt leaching by using underground salt water or natural rainfall to obtain salt-leached drained water with the salt content of 0.3-10 wt.%; and leading the salt-leached drained water in the microalgae cultivating device for microalgae cultivation. By means of the method and the system, the soil of the coastal saline and alkaline land can be effectively desalted, and simultaneously, the salt-leached drained water can be effectively used for cultivating microalgae.

The invention provides a method for using artificial wetlands for processing the waste water in circular seaculture, which comprises the following steps: the waste water is first filtered by a microstrainer and then led to an artificial wetland for treatment; the artificial wetland is in the form of a horizontal undercurrent packed bed and is divided into a front anaerobic zone and a back aerobic zone, the surface area ratio of which is 1-2:4-3, no plants are in the filler of the anaerobic zone while salt tolerant plants are planted in the filler of the aerobic zone; the effluent from the microstrainer sequentially flows through the anaerobic zone and the aerobic zone to be discharged, and the water flowing to the anaerobic zone contains the water flowing out of the aerobic zone. The artificial wetland provided by the invention is capable of seawater processing, denitrification, denitrogenation and dephosphorization, and can effectively process the suspended matters, organic matters, nitrogen, phosphorus and the like in the sewage flowing through the artificial wetland in multiple ways and cause the effluent quality to reach the water quality standard requirements of fishery industry of China, thus realizing the aim of recycling the effluent. Compared with the prior art, the invention has the advantages of good treatment effect, low cost and environment protection but no pollution.

The invention relates to the technical field of plant genetic engineering, and particularly discloses an application of a corn CIPK42 protein and a coding gene of the corn CIPK42 protein in regulationand control of salt stress tolerance of plants. The corn ZmCIPK42 gene is found to be able to positively regulate and control the salt tolerance of the plants; and the salt tolerance of the plants can be effectively improved by increasing of the expression quantity of the ZmCIPK42 gene. According to the invention, transgenic corn and arabidopsis thaliana plants with ZmCIPK42 overexpression are constructed; and compared with a non-transgenic wild type, the transgenic corn and arabidopsis thaliana plants are significantly improved in salt tolerance and growth. Discovery of the salt-tolerant function of the ZmCIPK42 gene provides a novel gene target and resource for cultivation of salt-tolerant plant varieties, is of great significance to research of a salt-tolerant molecular mechanism of the plants, and lays a certain theoretical foundation for research of a salt stress response mechanism of the plants and a molecular mechanism of resisting adverse environments.

Alfin1 cDNA encodes a putative transcription factor associated with salt tolerance in alfalfa (Medicago sativa L). The recombinant protein binds DNA in a sequence specific manner, including promoter fragments of the salt inducible gene MsPRP2. Alfin1 function was tested in transgenic alfalfa under the control of the 35S promoter in the sense and antisense orientations with the endogenous MsPRP2 as a reporter gene. Calli overexpressing Alfin1 were more resistant to growth inhibition by 171 mM NaCl than vector transformed controls, while calli expressing Alfin1 antisense were more sensitive to salt inhibition. Transgenic plants overexpressing Alfin1 in the sense orientation grew well. In contrast, the antisense transgenic plants grew poorly in soil, demonstrating that Alfin1 expression is essential for normal plant development. Transgenic calli and plant roots overexpressing Alfin1 showed enhanced levels of endogenous MsPRP2 mRNA accumalution. However, MsPRP2 mRNA accumulation was also regulated in a tissue specific manner as shown in leaves of transgenics overexpressing Alfin1. These results suggest that Alfin1 acts as a transcriptional regulator in plants and MsPRP2 expression in alfalfa. Alfin1 overexpressing transgenics showed salinity tolerance comparable to one of our salt-tolerant plants, indicating that Alfin1 also functions in gene regulation in salt tolerance.

The invention describes the preparation of nutrient-rich salt from high salt-accumulating and edible oil-bearing salt tolerant plants in a way that allows simultaneous recovery of both salt and oil. The plants are routinely irrigated with seawater and occasionally with seawater enriched with salt bitterns and / or other types of wastes / by-products containing essential nutrients to raise the level of such nutrients in the plant.

The invention relates to a topsoil salt-control method for planting a salt-tolerant plant in mudflat kaline soil, which comprises the step of determining the freshwater salt-sprinkling watering amountaccording to the salinity content background of soil, a salt-tolerant threshold value of a planted plant, a slat-control target value, actual measured data of salinity dynamic state, actual measureddata of the water content of the soil, a predicted value of rainfall and evaporation within the past few days and soil permeability. The method is characterized in that a four-electrode salinity sensor and a digital soil water potential monitor are adopted to monitor the water-salt dynamic state of the topsoil in real time, one monitoring site is arranged per 1-10 mu, and monitoring signals collected by each monitoring site are intensively processed, thereby realizing the overall real-time monitoring of an irrigation process and a salt leaching effect.

The invention discloses application of a glycosyltransferase gene UGT85A5 of Arabidopsis thaliana to the improvement of salt tolerance of plants. A nucleotide sequence of the glycosyltransferase gene UGT85A5 is shown as SEQ ID No.1; and the glycosyltransferase gene UGT85A5 is cloned from the Arabidopsis thaliana by a reverse transcription-polymerase chain reaction (RT-PCR) technology. The gene UGT85A5 is utilized to construct a plant overexpression vector and perform transgenic operation of the plants to obtain transgenic plants. The detection shows that the salt tolerance of the transgenic plants is remarkably improved, so that a novel salt-tolerant plant can be created after the invention is implemented; and the gene can be used for subsequent crop variety improvement, and has a great significance for the agricultural production in China.

The subject invention includes non-naturally occurring salt tolerant plants that accumulate sodium in their tissues substantially in the vacuole. The present invention also includes methods of making such non-naturally occurring plants. One preferred method is generating a transgenic plant that has an ectopically expressed NHX related gene product.

The invention discloses an improvement method of soil ecological depth in a moderate saline-alkali land. The improvement method comprises the steps that one, area surface sundries and weeds of the saline-alkali land to be improved are cleared; two, composite biological ameliorant is spread on the surface of the saline-alkali land to be improved and mixed with sand, carbide slag and potassium humate, a large quantity of soil beneficial microorganisms are bred in the short term, and soil activity is rapidly activated; three, the saline-alkali land to be improved is subjected to ploughing, leveling, irrigation, and film covering, then heat preservation and humidification for 10-15 days, acid-base balance is regulated while the soil salinity and alkali content is reduced, so that the microorganisms are over propagated and suffocated to death, and a sterile soil environment is created; four, membrane is removed and soil is air-dried, fermented organic fertilizer is applied, then salt-tolerant plants are sown to improve the biological depth of soil; five, when the growth of the sown salt-tolerant plants reaches the maximum, the salt-tolerant plants are removed and moderate saline-alkalisoil improvement is completed. The method is low in improvement cost, good in effect, and ecological and environmentally friendly.

The present inventions relate to compositions and methods for providing stress tolerant transgenic plants comprising a RING domain zinc-finger motif transcription factor protein. More particularly, the invention relates to compositions and methods comprising a RING-H2 domain transcription factor protein for providing drought and salt tolerant plants, in particular comprising a recombinant XERICO gene and protein.

The invention discloses a salt tolerant gene of wheat, namely CHP zinc finger protein gene TaCHP of wheat and a plant expression vector containing the gene TaCHP. The invention also discloses an application of the gene TaCHP to culturing a salt tolerant plant, in particular culturing the wheat. Experiments prove that the salt tolerant ability of the transgenic plants of the invention is improved obviously.

The invention belongs to the technical field of genetic engineering application, and in particular relates to an application of an arabidopsis At4g09820 gene in an aspect of breeding a new variety of salt resistant plants. The arabidopsis At4g09820 gene, which is called TT8 gene as well, is 1557bp in CDS length and capable of coding protein of 518 amino acids, belongs to a bHLH (basichelix-loop-helix) transcription factor and used for regulating biosynthesis of procyanidine and anthocyanin in a flavonoid synthetic route. The inventor, through research of the At4g09820 gene in a high-salinity adverse situation, discovers that the gene can specifically regulate and control an ion toxic reaction caused by NaCl and show an excellent salt resistance property; therefore, the At4g09820 gene has an important application in the plant salt resistance aspect, and an excellent salt resistant plant variety can be bred through further research and transformation on the gene.

The invention describes the preparation of nutrient-rich salt from high salt-accumulating and edible oil-bearing salt tolerant plants in a way that allows simultaneous recovery of both salt and oil, the plants are routinely irrigated with seawater and occasionally with seawater enriched with salt bitterns and / or other types of wastes / by-products containing essential nutrients to raise the level of such nutrients in the plant.

The invention relates to a breeding method for culturing salt-tolerant plants. The method comprises the steps of selecting quality full seeds; determining the salt tolerance range of the plants selected; preparing a culture medium of which the total salt content is the same as the upper limit of the salt tolerance range of the plants selected; planting the plants in the culture medium within the upper limit of the salt tolerance range; observing the development and growth conditions of the seeds after at least 14 days of planting; timely removing dead seedlings and maintaining well-growing seedlings; naturalizing the salt tolerance; gradually improving the total salt content of the culture medium according to the method in step (4); naturalizing to obtain new variety which can normally grow in the culture medium with high total salt content. With the adoption of the method, the salt-tolerant variety can be sieved within a short period; the method is small in cost, simple and convenient to operate, and high in breeding efficiency. The formulas are shown in the specification.

The present invention discloses ecological slope protection precast blocks and a preparation method and application thereof. The ecological slope protection precast blocks comprise the following raw materials: riverway sludge, a curing agent, plant source fibers and microorganism capsules, wherein the curing agent is a mixture of quick lime, fly ash, magnesium oxide and calcium sulfate; the plantsource fibers are plant straws; the microorganism capsules are formed by embedding functional microorganisms in urea formaldehyde resin; and the functional microorganisms are trichoderma reesei T131 and bacillus subtilis D9. The sludge, the plant source fibers and the microorganism capsules are cured by the curing agent to prepare the ecological slope protection precast blocks with the effects ofintercepting water body pollutants, performing field planting of plants and protecting slopes. Salt-resistant plant seeds are pre-attached in the ecological slope protection precast blocks or the salt-resistant plant seeds are sprayed to the surfaces of the precast blocks after the ecological slope protection precast blocks are paved. When the ecological slope protection precast blocks are used for tidal marsh silty bank slopes which are liquefied by meeting water and are collapsed by rainfall, the sewage nitrogen interception rate reaches 15-55%, the field planting success rate of the plantsis improved by 10-30 times, and the plant biomass is improved by 5-10 times.

The invention discloses a wheat salt-tolerant gene pyruvic acid transporter gene, and particularly relates to an allene oxide reductase gene TaBASS2 and a plant expression vector containing the gene TaBASS2. The invention further discloses an application of the gene TaBASS2 in cultivation of salt-tolerant plants, especially wheat. An experiment proves that the salt tolerance of a transgenic plant prepared by transgene transformation disclosed by the invention is obviously improved; and a foundation is laid for wide application of the gene in cultivation of a new variety of salt-tolerant crops.

The invention discloses a mutation and screening method for salt-tolerant buckwheat mutants. The method comprises the following steps: treating and mutating buckwheat seeds by adopting EMS (Ethyl Methyl Sulfone), screening buckwheat mutants insensitive to ethylene by virtue of a direct precursor ACC (1-aminocyclopropane-1-carboxylic acid) obtained through ethylene synthesis, cultivating the buckwheat mutants into saline-alkali soil with the salinity of 0.4 to 0.6 percent, screening salt-tolerant plants, planting harvested buckwheat seeds into the saline-alkali soil with the salinity of 0.4 to 0.6 percent, and further screening the salt-tolerant buckwheat mutants with stable characters. According to the mutation and screening method for the salt-tolerant buckwheat mutants, the mutants are easily obtained by mutation with the EMS, DNAs are less damaged by point mutation, and meanwhile, screening with the direct precursor ACC in combination with ETH is combined to screen the salt-tolerant buckwheat mutants, so that the mutation frequency is high, the target is clear, the screening efficiency is high, and can reach 10 to 20 percent, the operation cost is low, and the obtained mutants have stable characters.

The invention belongs to the technical field of sewage treatment, and discloses a sewage purification device applied to the eutrophic river estuary. The device includes a floating bed frame and a power mechanism. An aerator and an air guide pipe connected with the aerator are arranged on the floating bed frame. The inner side of the floating bed frame is connected with a plurality of parallel PVCpipes. The PVC pipes are connected with the air guide pipe, aquaculture grids are arranged between the PVC pipes at intervals, and the salt-tolerant plants are planted on the aquaculture grids. Aeration pipes are arranged on the lower portions of the aquaculture grids at intervals, one end of each aeration pipe is communicated with the corresponding PVC pipe, and a counterweight ball is tied to the other end of the each aeration pipe; four corners of the floating bed frame are provided with support columns, the support columns are connected with ropes, and the free ends of the ropes are fixedon the river bank. The eutrophication problem of a water body of the river estuary is mainly solved by the bioremediation and aeration technology, the device is simple in structure, light and good inanti-corrosion performance, a frame body can change along with the fluctuation of tide and rotate and move by itself, solar energy and rainwater are used for generating electricity, and energy conservation and environment protection are achieve.

The invention belongs to the field of gene engineering and discloses a helianthus tuberosus fructan-exohydrolases (Ht-FEH) gene and application of the Ht-FEH gene. The gene Ht 1-FEH has a nucleotide sequence shown in the SEQ ID NO.1 and is used for coding Ht 1-FEH with an amino acid sequence shown in the SEQ ID NO.2. The gene Ht 1-FEH can be applied to production of fructose substances through hydrolysis of inulin or cultivation of transgenic salt-tolerant plants.

The invention discloses a salt-tolerant gene for wheat, a plant expression vector and particular application of the gene TaAOC1. The salt-tolerant gene is allene oxide reduction enzyme gene TaAOC1 and is applied to cultivating salt-tolerant plants, in particular to cultivating wheat, and the plant expression vector comprises the gene TaAOC1. The salt-tolerant gene TaAOC1, the plant expression vector and the application have the advantages that as proved by experiments, the salt tolerance of transgenic plants which are subjected to transgenosis by the aid of the salt-tolerant gene is obviously improved, and a foundation is laid for widely applying the gene to cultivating new species of salt-tolerant crop.

The invention relates to the field of improvement technologies and ecological remediation of saline-alkaline land, in particular to a method for ecological re-greening of coastal saline-alkaline sandby using salt-tolerant plants. A saline-alkaline isolation layer consists of broken brick residues or miscellaneous fill and coarse sea sand, loose large-porous structures formed by the miscellaneousfill and the broken brick residues in constructional engineering can effectively block salt in lower layers from returning to the surface layer of soil along with water under the capillarity action, infiltration discharge of salt on the upper layer can be dredged, construction waste can be used, and the construction cost can be saved; a salt reverse osmosis layer can effectively block root systemsof the plants from penetrating the isolation layer, prevent the root systems from being affected by salt damage and prevent reverse osmosis of salt of the isolation layer; the arrangement principle of a saline-alkaline sand improvement layer lies in that physical and chemical properties of the saline-alkaline sand are improved, and effects of soil looseness and breathability, water retention, sufficient fertility and proper pH can be realized by cooperation with mushroom soil. The technical scheme has active and significant effects on ecological re-greening of the coastal saline-alkaline sandand is suitable for promotion on a large scale.