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Mutation and screening method for salt-tolerant buckwheat mutants

A technology for mutagenesis screening and mutants, applied in the directions of botanical equipment and methods, applications, plant gene improvement, etc., can solve the problem of no mutagenesis screening of buckwheat salt-tolerant mutants, and achieve high screening efficiency and high mutagenesis frequency. , targeted effect

Inactive Publication Date: 2015-04-29
QINGDAO AGRI UNIV
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Problems solved by technology

However, there is still no report on mutagenesis screening of buckwheat salt-tolerant mutants

Method used

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  • Mutation and screening method for salt-tolerant buckwheat mutants
  • Mutation and screening method for salt-tolerant buckwheat mutants

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Embodiment 1

[0018] (1) Pretreatment: Select buckwheat seeds with full grains, wash them with tap water first, then shake and wash them with deionized water for 3 hours, and blot them dry with absorbent paper; then soak them in 0.4% EMS solution for 10 hours, and finally wash them with 100mmol / L PBS (pH 7.5) rinse 2 times, deionized water rinse 3 times;

[0019] (2) Screening of buckwheat mutants: screening of buckwheat mutants insensitive to ETH on MS solid medium containing 20 μmol / L ACC, that is, mutants without "triple reaction"; experiments showed that on the medium added with ACC The wild-type buckwheat seedlings were short, with stubby stems; while the mutant buckwheat seedlings were taller, with slender stems, and were insensitive to ETH.

[0020] (3) Screen buckwheat salt-tolerant mutants: Buckwheat mutants are cultivated in saline-alkali soil with a salinity of 0.6%, and salt-tolerant plants are screened and seeds are harvested; the harvested buckwheat seeds are replanted in sali...

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Abstract

The invention discloses a mutation and screening method for salt-tolerant buckwheat mutants. The method comprises the following steps: treating and mutating buckwheat seeds by adopting EMS (Ethyl Methyl Sulfone), screening buckwheat mutants insensitive to ethylene by virtue of a direct precursor ACC (1-aminocyclopropane-1-carboxylic acid) obtained through ethylene synthesis, cultivating the buckwheat mutants into saline-alkali soil with the salinity of 0.4 to 0.6 percent, screening salt-tolerant plants, planting harvested buckwheat seeds into the saline-alkali soil with the salinity of 0.4 to 0.6 percent, and further screening the salt-tolerant buckwheat mutants with stable characters. According to the mutation and screening method for the salt-tolerant buckwheat mutants, the mutants are easily obtained by mutation with the EMS, DNAs are less damaged by point mutation, and meanwhile, screening with the direct precursor ACC in combination with ETH is combined to screen the salt-tolerant buckwheat mutants, so that the mutation frequency is high, the target is clear, the screening efficiency is high, and can reach 10 to 20 percent, the operation cost is low, and the obtained mutants have stable characters.

Description

technical field [0001] The invention relates to a mutagenesis screening method for salt-tolerant mutants of crops, in particular to a mutagenesis screening method for buckwheat salt-tolerant mutants. Background technique [0002] Buckwheat is a short-season Polygonaceae crop suitable for growing in cold climates. It has short growth period, cold resistance, barren tolerance, wide adaptability and rich nutrition. The cultivated species of buckwheat mainly include Common Buckwheat and Tartary Buckwheat. (Tartary Buckwheat) Two species. Buckwheat is mainly planted in Northwest China and North China, where there are many saline-alkali lands, and the arid soil is seriously salt-returned, which brings great difficulties to the production of buckwheat. Therefore, it is of great significance and application value to study and develop the mutagenesis screening method of buckwheat salt-tolerant mutants, thereby expanding the polymorphism of buckwheat, and further breeding and develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
Inventor 杨洪兵董春海裴海霞
Owner QINGDAO AGRI UNIV
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