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Green tangerine orange peel reference extract and application thereof

A technology of extract and Qingpi, which is applied in the field of comparison extract of Qingpi to achieve the effect of ensuring consistency, stable properties and ease of use

Active Publication Date: 2017-06-13
广州浩意万医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because of the above-mentioned specific requirements for the reference extract of traditional Chinese medicine, there is no or immature green bark reference extract product prepared by using the green bark medicinal material as a raw material in the current prior art

Method used

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  • Green tangerine orange peel reference extract and application thereof
  • Green tangerine orange peel reference extract and application thereof
  • Green tangerine orange peel reference extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] Embodiment 1: the preparation of Qingpi reference extract

[0113] The present embodiment provides the preparation method of Qingpi reference extract of the present invention, and its method flow chart is as follows Figure 21 shown.

[0114] 1: Screening of raw materials and medicinal materials

[0115] In this step, the combination of thin layer chromatography and high performance liquid chromatography is used to screen the raw medicinal materials.

[0116] 1 Thin-layer chromatography screening of raw materials

[0117] 1.1 Preparation of the test product

[0118] Preparation of chemical reference solution: take hesperidin, nobietin, and tangeretin reference substances, accurately weigh them, and prepare 0.1 mg / ml solutions with methanol respectively.

[0119] The preparation of medicinal material need testing solution: get green peel A, green peel B, green peel C, green peel D, green peel E, green peel F, green peel G, green peel H, green peel I, green peel J med...

Embodiment 2

[0242] The character analysis of embodiment 2 Qingpi contrast extract

[0243] 1. Appearance: The Qingpi control extracts QPERS-1 and QPERS-2 obtained in Example 1 are both yellow-green dry powders.

[0244] 2. Moisture determination: Carry out according to Appendix IX G of the Chinese Pharmacopoeia of the 2010 edition (reduced pressure drying method). The test result shows that the water content of QPERS1 is 2.70%, and the water content of QPERS2 is 2.30%.

[0245] 3. Consistency test: prepare 10 batches of Sihua Qingpi ERS (QPERS-1) (6 batches) and a Qingpi ERS (QPERS-2) (4 batches) according to the method of Example 1, and each batch is measured The TLC detection results for flavonoid glycosides and flavonoid aglycone components showed little difference, and the fluorescent spots of hesperidin, tangeretin and nobiletin were all clearly displayed, and the distribution was very consistent; The content of hesperidin was detected by liquid chromatography within the standard r...

Embodiment 3

[0253] This example provides the application of the reference extract of Pseudomonas chinensis in Example 1.

[0254] Commercial medicinal materials were analyzed by thin-layer chromatography and high-performance liquid chromatography, respectively, with chemical reference substance and Qingpi reference extract as reference substances.

[0255] 1 TLC

[0256] 1.1 Sample preparation

[0257] Chemical reference solution: Accurately weigh 2.5mg each of hesperidin, nobiletin, tangeretin, neohesperidin, and naringin, put them in a 25ml volumetric flask, add 20ml of methanol, and process with ultrasonic (500w) for 15 minutes to Dissolve completely, let cool, make up to 25ml mark and pass through 0.22μm filter membrane.

[0258] Qingpi reference extract solution: Accurately weigh 20 mg each of Qingpi reference extracts QPERS-1 and QPERS-2 prepared in Example 1, put them in a 2ml measuring bottle, add 1.5ml of methanol respectively, ultrasonically (500w) for 15 minutes, put Cool, a...

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Abstract

The invention provides a green tangerine orange peel reference extract which is prepared by the following steps: carrying out multiple extraction on different sources or batches of green tangerine orange peel drug powders to obtain different sources or batches of green tangerine orange peel extracts, and blending the different sources or batches of green tangerine orange peel extracts to obtain the green tangerine orange peel reference extract. The green tangerine orange peel reference extract is blended from different sources or batches of extracts, thereby ensuring the consistency of different batches of green tangerine orange peel reference extracts. By selecting the representative drug as the raw material, the obtained product is representative, and can reflect the quality of the detected drug more visually when being used as the reference extract. The green tangerine orange peel reference extract has stable and uniform properties, and is convenient to use. The green tangerine orange peel reference extract can be used as the reference in the quality control of the green tangerine orange peel and drug preparations containing green tangerine orange peel, can be used for qualitative identification on the drug or traditional Chinese medicine preparation, and can also be used for semiquantitative and even quantitative analysis.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a reference extract of Qingpi and its application. Background technique [0002] Qingpi is the pericarp of dried young fruit or immature fruit of Rutaceae plant orange and its cultivars. From May to June, the young fruit that falls from the sun is collected and dried, which is commonly called "Geqingpi"; from July to August, the immature fruit is harvested, cut into four petals on the pericarp to the base, remove all the petals, and dry in the sun , Xi called "Sihua Qingpi". [0003] Qingpi has a long history of medicinal use, and is commonly used as a medicine for soothing the liver and breaking qi, eliminating accumulation and stagnation. In the 2015 edition of the Chinese Pharmacopoeia Quality Standard issued by the National Pharmacopoeia Committee, the content of hesperidin, one of the active ingredients of green peel, is stipulated:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/90
CPCG01N30/02G01N30/90
Inventor 郭隆钢许舜军孙帅许艺镌巫少娟赵岳锐谢培山许铮弟
Owner 广州浩意万医药科技有限公司
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