The invention discloses novel marine bacterium derived thermal-stable and salt-tolerant
organic solvent SGNH family hydrolytic
enzyme AlinE4 as well as a coding
gene and an application of the hydrolytic
enzyme. The hydrolytic
enzyme aline4 provided by the invention comes from Altererythrobacter indicus DSM18604, and a
nucleotide sequence of the hydrolytic enzyme aline4 is shown as SEQ ID NO.1. Thehydrolytic
enzyme gene provided by the invention, after undergoing heterogeneous expression, has
esterase activity, and the maximum catalytic activity, namely 25.8U / mg, is achieved when p-nitrophenolbutyrate (C4) is taken as a substrate. The optimum
catalytic hydrolysis pH and temperature of the AlinE4 are 7.5 and 40 DEG C; the AlinE4, when bred at 40 DEG C, 50 DEG C and 60 DEG C for 60min, canstill keep the activity above 50%; and with the presence of an
organic solvent and
metal ions, relatively high enzymatic activity is kept. The hydrolytic enzyme has the characteristics of being thermal-stable and alkaline; and the hydrolytic enzyme is applicable industrial production in such fields as detergents,
wastewater treatment,
fine chemical engineering,
pharmacy, environmental remediationand the like under the conditions of high temperature and containing salt and organic solvents.