62results about How to "Great application advantage" patented technology

The invention discloses a coating size composition for a thermosensitive discoloring and energy storing textile and a coating preparation method. The coating size composition comprises the following components in parts by weight: 100 parts of waterborne polyurethane or polyacrylate adhesive and associative polyurethane thickener or polyacrylic acid synthetic thickener for adjusting viscosity, and 5 to 50 parts of thermosensitive discoloring and energy storing microcapsule powder. The coating preparation method comprises that the thermosensitive discoloring and energy storing microcapsule powder is used as a raw material, the waterborne polyurethane or polyacrylate adhesive is used as a coating finishing agent, and the thicker is adopted to adjust the viscosity, so that the coating size can be prepared; and then the coating size is uniformly coated on the surface of the textile in a way of direct blade coating, and the textile is subject to pre-baking and baking processes so as to obtain the thermosensitive discoloring and energy storing textile. The prepared textile has dual functions of thermosensitive discoloring and energy storing, thus having relatively wide application prospect.

The invention discloses recombinant bacillus subtilis producing micromolecular hyaluronic acid and belongs to the technical field of bioengineering. Hyaluronic synthase hasA originated from streptococcus zooepidemicus is integrated into bacillus subtilis to realize production of hyaluronic acid and meanwhile, overexpression on a synthetic route key gene of HA is carried out to realize high yield of HA in bacillus subtilis. On the basis, hyaluronidase originated from leeches is integrated into a bacillus subtilis genome for secretory expression subjected to a promoter Pgrac under induction control of IPTG (isopropyl-beta-d-thiogalactoside). Different micromolecular hyaluronic acid products with different molecular weights are prepared by controlling the secretory expression amount of hyaluronidase and induction starting time. The prepared products are different in ranges of molecular weights and the recombinant bacillus subtilis has important guiding reference meaning for direct production of micromolecular hyaluronic acid in a special particular range by microorganisms. The recombinant bacillus subtilis disclosed by the invention lays a certain foundation for efficiently preparing the micromolecular hyaluronic acid and is suitable for industrial production and application.

The invention discloses a method for preparing small-molecule oligomeric hyaluronic acid through an enzyme method, belonging to the technical field of bioengineering. According to the invention, by using hirudo-derived hyaluronidase, through hydrolysis of high-molecule hyaluronic acid, oligomeric hyaluronic acid having specific small molecular weights of tetrasaccharide, hexasaccharide, octasaccharide, decasaccharide and the like can be efficiently prepared. The method disclosed by the invention lays a certain foundation for efficient preparation of specific small-molecule oligomeric hyaluronic acid, and is applicable to industrial production.

The invention discloses recombinant Bacillus subtilis for producing chondroitinase and application thereof and belongs to the technical field of bioengineering. Chondroitinase of common Proteus origin is heterologously expressed in Bacillus subtilis, signal peptide amyX is selected, 3 strong constitutive promoters and strong RBS (ribosome binding site) of pP43NMK vector itself are utilized, histidine tags are added to facilitate subsequent purification, and expressive enzyme activity of chondroitinase in Bacillus subtilis is improved. Certain basis is laid for efficiently producing chondroitinase from food-grade microbes, and the recombinant Bacillus subtilis is suitable for industrial application.

The invention discloses a coal property measuring method based on wavelet transform. The method comprises the following steps: firstly, detecting a coal calibration sample with known coal property by using an LIBS (Laser Induced Breakdown Spectroscopy) system, then performing wavelet de-noising processing on an LIBS spectrum obtained by detecting and obtaining a processed wavelet coefficient, and establishing a partial least square calibration model by taking the processed wavelet coefficient as an independent variable and taking a coal property value of the calibration sample as a dependent variable; and performing the wavelet de-noising processing on the LIBS spectrum of the sample to be measured, substituting the wavelet coefficient into the partial least square calibration model and calculating a coal property result of the sample to be measured. Through the method, environment noises and background noises are separated from original signals by using a wavelet tool, so that the interference of the LIBS analysis caused due to the noise is reduced, a signal to noise ratio of a characteristic spectral line is increased, and the measurement precision of the calibration model is also improved.

The invention discloses a BIM-based design calculation construction full life cycle application method. The method comprises the following steps of performing three-dimensional design by applying BIMsoftware revit; after the construction drawing model is completed, directly applying the design model to carry out BIM calculation; performing engineering setting, floor setting, mapping setting and engineering feature setting by applying BIM calculation amount software; performing three-dimensional model mapping on the Revit model, and determining and modifying a mapping result; setting an engineering quantity output form, a deduction rule, an engineering quantity priority output sequence and the like according to a corresponding quota rule in the calculation rule; The part which is not completely drawn of the model can be intelligently arranged for the second time; performing summary calculation; Exporting a three-dimensional calculation result into a calculation detail table and a calculation summary table; Uploading the Revit model to a construction management platform, and previewing the basic model on the platform. According to the method, the Revit model is used for carrying outengineering quantity statistics, the calculated quantity result meets the actual construction requirement, the personnel cost and the time cost are saved, and rapid and accurate quantity output can be achieved.

The invention discloses a guiding method of a robot bird, which adopts a proper micro-stimulation signal to timely stimulate an abdominal dorsal median nucleus and a robustus archistriatalis of a thalamencephalon of the bird to lead an active escaping behavior of the bird; thereby guiding the flying behavior of the bird. The invention utilizes the active escaping behavior of the bird resulted from using a micro-electricity to simulate a specific nerve nuclei of the bird, namely using a principle of using the micro-electricity to simulate the nerve nuclei to image 'hurt' to guide the behavior of the bird. The method used for guiding the robot bird of the invention has the advantages that as the principle utilizes the appetence of the bird to avoid hurt, the invention has a powerful guiding function; thereby needing not to train the bird in advance; the robot bird has a huge using value; compared with the ground animal, the bird has a larger active space and fast moving speed, thus having a larger application advantage obviously; the realistic and potential application is very wide.

The invention discloses a recombinant Pichia pastoris for producing small-molecular hyaluronic acids and a construction method thereof, belonging to the technical field of bioengineering. Hyaluronic acid synthase hasA derived from Streptococcus zooepidemicus and UDP-glucose dehydrogenase tuaD derived from Bacillus subtilis are expressed in recombinant Pichia pastoris GS115 host to implement production of hyaluronic acids. Meanwhile, hyaluronidase derived from hirudo is integrated to the Pichia pastoris genome and subjected to secretion expression by constitutive promoters with different intensities; the secretion expression level of the hyaluronidase is controlled to prepare small-molecular hyaluronic acid products with different molecular weights; and the prepared products have different ranges of molecular weight, and have instruction and reference meanings for directly producing small-molecular hyaluronic acids within a specified range from microbes. The recombinant Pichia pastoris lays certain foundation for high-efficiency preparation of small-molecular hyaluronic acids, and is suitable for industrial production.

The invention relates to a quasi-planar high-isolation multi-path power divider. An input radio-frequency signal is subjected to multi-path power allocating and input impedance matching through an upper-level power dividing network. Meanwhile, the insulating characteristics and the output VSWR at all output ports are ensured to be excellent through a lower-level isolating network and an isolation resistor. The upper-level power dividing network and the lower-level isolating network are connected via metallized through holes. According to the technical scheme of the invention, the advantages of a quasi-planar structure, including multiple ports, high isolation, low insertion loss, good input / output standing wave ratio, wide band, small volume, good consistency of signal amplitudes / phases at all output ports, and the like, are realized. Meanwhile, the quasi-planar high-isolation multi-path power divider is convenient to design and the infinite multi-path power distribution can be realized theoretically. The quasi-planar high-isolation multi-path power divider is mainly applied to microwave and millimeter wave power synthesis and amplification systems, phased array antenna feed networks and the like. The quasi-planar high-isolation multi-path power divider has a wide application prospect in the fields of microwave and millimeter wave systems for communication, radar, measurement, control and the like.

The invention provides a new application of a coordination compound of flavone and trace elements as animal feedstuff additives. The coordination compound of flavone and trace elements can be used as a growth promoting agent, an anti-diarrhoea agent, an antioxidant and an immunity promoting agent to effectively supplement the trace elements for animals, promote animal healthy growth and improve feedstuff utilization ratio, and has the effects of growth promotion, anti-diarrhoea, anti-oxidation, immunity improvement and the like. Moreover, drug residues left in animal by-products caused by antibiotics are avoided. Because the dosage of the coordination compound is low, the pollution of animal excrement to the environment is reduced. The coordination compound of the flavone and the trace elements has the advantages of promoting growth, improving feedstuff efficiency, replacing antibiotics as the growth promoting agent, eliminating drug residues of antibiotics in products for animals, and reducing environmental pollution, and can be used for pollution-free animal product production.

The invention discloses a common sow thistle fermentation preparation method, belongs to the field of vegetable deep-processing and particularly relates to a method for producing fermented common sow thistle by using lactic acid bacteria powder. The method solves the technical problem of the quick fermentation production of the common sow thistle. The invention mainly comprises: (1), a step of bacterium addition and mixing, in which the common sow thistle is washed, cut and then placed into a container, a lactic acid bacteria agent is added in an amount of 0.04 to 0.3 percent based on the weight of the common sow thistle, edible salt in an amount of 1 to 3 percent and sugar in an amount of 1 to 2 percent and water are also added into the container and then the container is sealed; (2) a step of fermentation, in which the temperature is kept between 20 and 36 DEG C to perform fermentation for 30 to 50 hours; and (3) a step of bacterium addition, seasoning, packaging, storage in refrigerators and direct sale or sale after pasteurization. The method is applicable to common sow thistle fermentation production of the different scales and can obviously shorten production time and ensures that the product has rich nutrients and a pure taste.

The invention discloses a hyaluronan synthase mutant and application thereof and belongs to the technical field of bioengineering. The mutant with positive effect in both yield and molecular weight is acquired by subjecting hyaluronan synthase of Streptococcus zooepidemicus origin to intracellular domain mutation in Bacillus subtilis; meanwhile, genes of UDP-GlcUA and UDP-GlcNAc synthetic routes in Bacillus subtilis are subjected to modular assembly expression analysis, and high yield of hyaluronan with wide molecular weight range from Bacillus subtilis is implemented by controlling different UDP-GlcA and UDP-GlcNAc concentrations and ratios. Certain basis is laid for the efficient preparation of hyaluronan from food-grade microorganisms, and this mutant is suitable for industrial production and application.

The invention discloses a broadband Doherty power amplifier based on a single parallel resonance block. The broadband Doherty power amplifier comprises a broadband input power divider; a carrier amplification circuit, which is mainly formed by sequentially cascading a phase shift network, a first input matching network, a carrier amplifier T1 and a first output matching network; a peak value amplifying circuit, which is mainly formed by sequentially cascading a second input matching network, a peak value amplifier T2 and a second output matching network; and a combined resonance circuit, whichcomprises a parallel resonance block and is mainly formed by connecting a transmission line inductor L1 and a lumped capacitor C1 in parallel, wherein the constraint condition M selected for the inductance value of the transmission line inductor L1 is that when the two ends of the parallel resonance block are connected with terminating loads with the impedance of Ropt, the S11 amplitude of the parallel resonance block is not larger than -15 dB, and the absolute value of the S21 phase is not larger than 10 degrees. The broadband Doherty power amplifier is low in insertion loss, compact in structure, smaller in overall size, beneficial to miniaturization design and capable of saving cost.

The invention discloses a preparation method for a large-specification manganese-containing high-entropy alloy cast ingot. The preparation method for the large-specification manganese-containing high-entropy alloy cast ingot comprises the following steps: weighing the weights of Fe, Cr, Ni and Mn according to the content of 25at.% of Fe, Cr, Ni and Mn elements in a Fe-Cr-Ni-Mn high-entropy alloy,and adding Fe, Cr, Ni and Mn into a crucible; then carrying out primary argon-filled vacuum induction smelting to obtain a primary cast ingot of the high-entropy alloy, then carrying out argon-filledconsumable electro-arc smelting twice, and finally carrying out vacuum heat treatment to obtain the large-specification manganese-containing high-entropy alloy cast ingot. In the aspect of preparationfor the high-entropy alloy, the alloy cast ingot is obtained by carrying out the primary argon-filled vacuum induction smelting, then carrying out the argon-filled consumable electro-arc smelting twice, and then carrying out the vacuum heat treatment; and the method is simple and practicable in a process for preparing a high-entropy material, high in efficiency, low in requirement on equipment, and low in cost. The high-entropy alloy cast ingot prepared by the preparation method is accurate in component and high in uniformity, and provides an excellent blank for the subsequent hot-processingprocedure.

The invention belongs to the technical field of novel materials, in particular to a preparation method of silver nanobelts. In the method, silver nitrate is taken as a metal salt precursor, water is taken as the solvent and aminoanisole is taken as the reducing agent to react in a hydrothermal reactor for 1-24 hours at 100-240 DEG C to obtain the silver nanobelts which have thickness of 20-100 nanometers, width of 100-1000 nanometers and length of 1-100 micrometers and are protected by poly-aminoanisole. The silver nanobelts prepared by the invention have controllable topography and adjustable size besides high specific surface, the characteristic of common nano materials, and are expected to be widely applied in such fields as conducting materials, electromagnetic shielding, static resisting, sterilization, surface enhanced Raman light, electricity and the like and novel composite materials.

The invention provides a preparation method and application of a bacillus cereus monoclonal antibody. The preparation method comprises the following steps of: 1, preparing bacterial antigen through fixation of bacillus cereus by incubation treatment of paraform; 2, injecting the bacterial antigen so as to avoid emulsification; 3, performing short spacing impulse injection so as to quickly promote immunization; 4, planting solid tumors so as to rejuvenate tumor cells; 5, performing chemical induction and fusion; 6, performing semifluid selection so as to obtain monoclonal hybridoma; 7, performing cooperation of negative screening and positive screening so as to obtain a specific antibody; and 8, using a polyclonal antibody as a capture antibody and using a monoclonal antibody as a detection antibody to establish dual-antibody sandwich ELISA.

The invention discloses a method for constructing recombinant bacillus subtilis with high-yield hyaluronic acid oligosaccharide, and belongs to the technical field of bioengineering. The method includes selecting yoyJ-yoyA, lytH, lytD, lytG and lytC as integration loci, and integrating pathway genes glmM, glmS, glum and tuaD and a leech-derived hyaluronan hydrolase gene H6LHyal into a bacillus subtilis genome, which not only improves the yield of hyaluronic acid oligosaccharide but also increases the biomass and facilitates separation and purification of the hyaluronic acid oligosaccharide inthe later stage. The yield of the hyaluronic acid oligosaccharide reaches 36.04g / L when fermented in a 3L fermentation tank for 40h. The hyaluronic acid at the 3L fermentation tank level has the average molecular weight HA Mw of about 3.3*104 Da. The method lays a foundation for high-efficiency fermentation of a microbial system to prepare the hyaluronic acid oligosaccharide, and is suitable for industrial production application.

The invention discloses wide-spectrum anti-reflective ultraviolet shielding cover plate glass for a double-glass component solar cell. The wide-spectrum anti-reflective ultraviolet shielding cover plate glass comprises a rolled glass substrate; a mixed dielectric film layer and an acidic SiO2 film layer are sequentially laminated on a top surface of the rolled glass substrate; the mixed dielectricfilm layer is prepared by mixing a TiO2 sol and a SiO2 sol and coating a film; the molar ratio of TiO2 sol to SiO2 sol is 0.3:1-6:1; the acidic SiO2 film is prepared by coating the SiO2 sol; the pH of SiO2 sol is 2.0-5.0; the SiO2 solid content is 0.1-0.3%; the pH value of the TiO2 sol system is 2.0-5.0; the TiO2 solid content is 0.1-0.5%; a composite film base layer is formed by TiO2 and SiO2. The precise control of the refractive index of the film can be implemented through control of the component ratio; the acidic SiO2 on the upper layer guarantees the hardness and bonding force of the film; the transmittance in the wide spectrum range is effectively increased, meanwhile the transmittance of ultraviolet light can be effectively reduced and the ultraviolet shielding is implemented.

The invention discloses a heparin precursor synthase and application thereof and belongs to the technical field of bioengineering. Heparin precursor synthases KfiC and KfiA from escherichia coli K5 are modified in three ways in bacillus subtilis, i.e., the two synthases are directly fused; the two synthases are connected through a flexible linker; and key areas in the KfiC and KfiA replace a key area of a PmHS synthase from pasteurella multocida to obtain three novel heparin precursor synthases, and the results that the yield of heparin precursors is improved and the molecular weight of the heparin precursors is reduced are realized. The invention lays a foundation for efficiently producing and preparing the heparin precursors from food grade microorganisms, and the heparin precursor synthase is suitable for industrial production and application.

The invention discloses a recombinant Pichia pastoris producing small molecule hyaluronic acid and a construction method thereof, belonging to the technical field of bioengineering. The present invention adopts hyaluronic acid synthase hasA derived from Streptococcus zooepidemicus and UDP-glucose dehydrogenase tuaD derived from Bacillus subtilis, expressed in the recombinant Pichia pastoris GS115 host to realize the production of hyaluronic acid; Hyaluronidase is integrated into the Pichia pastoris genome, secreted and expressed under constitutive promoters of different strengths, and small molecule hyaluronic acid products with different molecular weights are prepared by controlling the secretion and expression of hyaluronidase. There are differences in the molecular weight ranges of the products, which have important guiding significance for the direct production of specific ranges of small molecule hyaluronic acid by microorganisms. The invention lays a certain foundation for efficient preparation of small molecule hyaluronic acid and is suitable for industrial production and application.

The invention provides a preparation method of bacillus cereus polyclonal antibodies, which comprises the following steps: 1, enrichment: coating activated bacillus cereus on a flat plate for enlarge cultivation; 2, fixation: fixing surface compositions of the bacillus cereus by using a modified boiling water bath method, so that antigenic determinants are fully exposed; 3, emulsification: preparing a uniform emulsified liquid from an adjuvant-bacillus mixture by using a self-assembled emulsifier; 4, immunization: carrying out animal immunization in a mode of being based on multiple loca on the back and supplemented by peripheral loca; 5, antiserum titer: evaluating the antiserum titer by using an ELISA method, and carrying out enhanced immunization; 6, taking whole blood: taking whole blood from cervical arteries under the condition of anesthesia by using chloral hydrate; 7, preparation of antiserum: standing the rabbit whole blood at optimum temperature so as to carry out natural chromatography, and carrying out centrifugal separation on the antiserum; and 8, purification: purifying the antiserum by using an n-caprylic acid-ammonium sulfate method, so that a polyclonal antibody is prepared. The polyclonal antibody prepared by using the preparation method disclosed by the invention is high in titer, good in specificity and strong in sensitivity, and has a great application advantage.

The invention relates to a dual laser type robot joint angle measuring method and angle measuring device. The measuring method comprises the steps: building a laser-plane angle measurement model through employing a ground plane as a reference plane, and obtaining the rotation angle of a robot joint through the measurement of a dual laser distance measurement change value at the robot joint; the measuring device comprises a dual laser range finder which is installed on the robot, and the central axis of the dual laser range finder is parallel to the arm axis of the robot; and a computer is connected with the dual laser range finder, collects left and right laser range finding values of the dual laser range finder in real time in the movement process of the robot joint, and obtains the rotation angle of the robot joint based on the laser-plane angle measurement model. Compared with the methods and the devices in the prior art, the method and the device have the advantages of high measurement accuracy, simplicity and the like.

The invention discloses constitutive-inductive type yeast engineering bacteria and construction method thereof. The method includes steps of: connecting a constitutive expression cassette and an inductive expression cassette, which simultaneously include protein genes requiring expression, in series and leading the expression cassettes into a yeast host cell; performing antibiotics screening justfor one time to obtain the yeast engineering bacteria simultaneously having constitutive and inductive expression. For an example of expression of beta-mannase, compared with engineering bacteria in single constitutive or inductive expression for expression of the beta-mannase, the constitutive-inductive type yeast engineering bacteria is significantly improved in fermentation activity of the beta-mannase. The method is simple in operation and can be used for increasing expression level of other heterologous proteins in yeast engineering bacteria.

The invention belongs to the technical field of novel materials, in particular to a preparation method of silver nanobelts. In the method, silver nitrate is taken as a metal salt precursor, water is taken as the solvent and aminoanisole is taken as the reducing agent to react in a hydrothermal reactor for 1-24 hours at 100-240 DEG C to obtain the silver nanobelts which have thickness of 20-100 nanometers, width of 100-1000 nanometers and length of 1-100 micrometers and are protected by poly-aminoanisole. The silver nanobelts prepared by the invention have controllable topography and adjustable size besides high specific surface, the characteristic of common nano materials, and are expected to be widely applied in such fields as conducting materials, electromagnetic shielding, static resisting, sterilization, surface enhanced Raman light, electricity and the like and novel composite materials.

The invention provides a chloramphenicol efficient degrading bacterium, an efficient degrading bacterium agent and application thereof, and belongs to the technical field of biodegradation. The classification name of the chloramphenicol efficient degrading bacterium is Shewanella algae, the code is SA-cd1, and the chloramphenicol efficient degrading bacterium has been preserved in the China General Microbiological Culture Collection Center with the preservation number being CGMCC No.21690. The strain is separated and screened from paphia undulata, and can be prepared into the bacterium agent to be applied to chloramphenicol degradation. The degrading bacterium is extremely high in chloramphenicol degradation efficiency and wide in degradation condition, metabolites of the degrading bacterium can be independently and rapidly degraded, growth and metabolism of thalli do not need to be depended on, and the application advantages and value are large.

The invention provides a bait feeding method for improving the industrialized larva breeding survival rate of freshwater shrimps. In a freshwater shrimp larva production process, artificial baits and biological baits are mixed according to a certain ratio and fed, and the nutrient is more comprehensive than that of single feeding of frozen and fresh biological baits. Furthermore, compared with a manner of completely feeding the artificial baits, the pollution to a breeding water body is extremely effectively reduced, the occurrence probability of sessilinasis and filamentous bacteria is reduced, and the survival rate of larva breeding is improved and can reach 47.2 percent at maximum; in addition, the cost is saved by 40 percent or more when being compared with the manner of completely feeding the artificial baits. The method provided by the invention is a method capable of improving the larva breeding survival rate of the freshwater shrimps and reducing the production cost; especially, the bait feeding method is suitable for freshwater shrimp larva production of the Northern inland region in China and has relatively high popularization value.

The invention belongs to the technical field of remote sensing, and particularly relates to an MTF-based remote sensing image quality evaluation method. The method comprises the following steps: step1, selecting a calculation area; step 2, carrying out straight line fitting on the step edge of the image; 3, fitting an edge spread function; 4, performing deriving to obtain a line spread function;and step 5, performing Fourier transform to obtain an MTF. The method solves the problem of objective evaluation of the remote sensing image. According to the method, the remote sensing image can be objectively evaluated, and the problems that a subjective evaluation method depends on testers to manually interpret the remote sensing image, time and labor are wasted, and automatic interpretation ofmassive remote sensing data is not facilitated are solved. In the actual application of remote sensing image processing, a reference image corresponding to a distorted image cannot be obtained, and areference evaluation method is not suitable, so that the reference-free evaluation method based on MTF has greater application advantages and wider application range.

The invention discloses an enzymatic method for preparing small molecular oligomeric hyaluronic acid, which belongs to the technical field of bioengineering. The present invention uses hyaluronidase derived from leeches to efficiently prepare oligomeric hyaluronic acid with specific small molecular weights such as tetrasaccharides, hexasaccharides, octasaccharides and decasaccharides by hydrolyzing high-molecular hyaluronic acid. The invention lays a certain foundation for efficient preparation of specific small molecule oligomeric hyaluronic acid, and is suitable for industrial production and application.

The invention discloses a common sow thistle color-protected fermentation preparation method, belongs to the field of vegetable deep-processing and in particular relates to a method for producing fermented common sow thistle by using lactic acid bacteria powder. A protective agent consists of the following components in weight percentage: 60 to 80 percent of calcium carbonate, 10 to 25 percent of lemon and 10 to 16 percent of ginger. The method mainly comprises: (1), a step of bacterium addition and mixing, in which the common sow thistle is washed, cut and then placed into a container, a lactic acid bacteria agent is added in an amount of 0.04 to 0.3 percent based on the weight of the common sow thistle, edible salt in an amount of 1 to 3 percent and sugar in an amount of 1 to 2 percent and water are also added into the container and then the container is sealed; (2) a step of fermentation, in which the temperature is kept between 20 and 36 DEG C to perform fermentation for 30 to 50 hours; and (3) a step of bacterium addition, seasoning, packaging, storage in refrigerators and direct sale or sale after pasteurization. The method is applicable to common sow thistle fermentation production of the different scales and can obviously shorten production time and ensures that the product has rich nutrients and a pure taste.

The invention discloses a method for producing and preparing ultra-high molecular weight hyaluronic acid through a microbial fermentation method, and belongs to the technical field of bioengineering. According to the invention, a hyaluronic acid synthase encoding gene pmhasA is expressed in corynebacterium glutamicum in a free manner, and a synthetic route of hyaluronic acid is constructed; the expression of synthase pmHAS is weakened by adopting a method for weakening the strength of RBS; the hyaluronic acid synthetase encoding gene pmhasA is modified by adopting a strategy of site-specific mutagenesis; in the corynebacterium glutamicum, a synthetic pathway enzyme encoding gene of precursor UDP-glucuronic acid and UDP-N-acetylglucosamine is expressed in an enhanced manner through a combined regulation strategy; a fermentation process is optimized, and a low-temperature fermentation strategy is adopted; and the molecular weight of the hyaluronic acid produced by the recombinant corynebacterium glutamicum reaches 5-9 MDa. The recombinant corynebacterium glutamicum constructed by the invention is suitable for industrial production and application.