118 results about "Tetrasaccharide" patented technology

The invention discloses an enzymolysis-HPLC method for detecting enoxaparin. The method comprises steps of: a. complete enzymolysis of enoxaparin: adding a mixed enzyme solution with enzyme I, enzyme II and enzyme III in a ratio of 8:1:2 to an enoxaparin solution with a concentration of 10-200mg/ml and carrying out an enzymolysis at room temperature for 48 h. b. HPLC analysis: carrying out a SAX-HPLC analysis on the degradation products; c. calculation of variety and content of disaccharide and tetrose units: determining variety and content of disaccharide and tetrose units according to a wash out time of prior standard disaccharide and tetrose and calculating percentage content of each of the eight disaccharides and one tetrasaccharide.

A liquid pharmaceutical composition is contemplated that comprises a pharmaceutically effective amount of a drug dissolved or dispersed in an aqueous medium. The aqueous medium consists essentially of water, about 3% to about 10% w/v polyvinylpyrrolidone, about 60% to about 75% w/v of C₃-C₆ polyol that includes more than 55% w/v of a non-reducing disaccharide, trisaccharide or tetrasaccharide such as sucrose, optionally about 0.01% to about 0.5% w/v of a glycyrrhetic acid, glycyrrhizinate derivative or salt thereof, and one or more flavorants, and preferably includes one or more preservatives. The liquid composition is room temperature stable, and may have a pleasant taste.

The invention discloses a method for separating hyaluronate tetrasaccharide from hyaluronate hexasaccharide, and belongs to the technical field of bioengineering. The method comprises the following steps: adopting Q Sepharose fast flow as an ion exchanger, Tris-HCl with the pH value of 8-9 as an equilibrium liquid, and 0.125 M NaCl with the pH value of 8, performing linear gradient elution to separate hyaluronate tetrasaccharide from hyaluronate hexasaccharide, and desalting to obtain pure products of hyaluronate tetrasaccharide and hyaluronate hexasaccharide.

The object of the present invention is to provide a lipid-regulating agent or a composition for regulating the amount of lipids comprising the agent. The present invention solves the above object by providing a lipid-regulating agent comprising a cyclic tetrasaccharide and/or its saccharide-derivative(s) and a composition for regulating the amount of lipids comprising the lipid-regulating agent.

The invention discloses a preparation method of galacto-mannan-oligosaccharides and application of the galacto-mannan-oligosaccharides. The preparation method comprises the following steps: (1) performing segmented composite enzymatic hydrolysis on galacto-mannan-oligosaccharides such as guar gum, sesbania gum, locust bean gum, fenugreek gum and Tara gum, wherein the enzyme is a complex enzyme ofacid mannase and cellulase; and (2) by segmented ultrafiltration classification, obtaining degraded galacto-mannan-oligosaccharides which are a mixture of a disaccharide, a trisaccharide, a tetrasccharide and a pentosaccharide. The preparation process is simple and easy, and is environmentally friendly. The conversion rate of the galacto-mannan-oligosaccharides is high, and the galacto-mannan-oligosaccharides are easy to separate. The prepared galacto-mannan-oligosaccharides can be used as prebiotics to be applied to food and animal feed, the proliferation of a butyric acid-production probiotic is promoted, and the intestinal environment is improved.

The invention discloses a method for preparing kestose and nystose through yacon. Method of water extraction by alcohol sedimentation is adopted and supernate thereof is taken, concentrated, frozen and dried to obtain faint yellow yacon oligosaccharide. The yacon oligosaccharide is analyzed through high pressure liquid chromatography with a chromatography condition as follows: a chromatographic column is APS-2HYPERSIL, a column temperature is 30 DEG C, a moving phase is acetonitrile-water (75 to 25, V/V), a flow velocity is 0.8ml/min, a sample size is 20 Mul and a differential detector is arranged. The yacon oligosaccharide comprises fructose, glucose, saccharose, kestose, nystose and 1F-Fructofuranosyl nystose with contents respectively as 38,30 percent, 16.44 percent, 14.58 percent, 12.29 percent, 12.17 percent and 6.2 percent. Silicagel column chromatography is implemented on a prepared oligosaccharide sample; glacial acetic acid, chloroform, absolute ethyl alcohol and water with a proportion of 3 to 11 to 11 to 1 are used as an eluant for elution; the eluant is collected in sequence and then is concentrated, frozen and dried to obtain the kestose and nystose. The purity of the kestose and nystose prepared through high pressure liquid chromatography can be more than 90 percent.

The invention discloses a method for synthesis of chondroitin sulfate tetrasaccharide. The method comprises that orderly through enzymatic hydrolysis, protecting group protection, protecting group conversion, selective deprotection, hydroxyl oxidation and selective reduction, an intermediate compound shown in the formula O is prepared from hyaluronate as a starting material, and chondroitin sulfate tetrasaccharide is prepared from the intermediate compound shown in the formula O, wherein substituents in the formula O are specifically defined in the specification. The invention provides the intermediate compound for synthesis of chondroitin sulfate tetrasaccharide. Compared with the existing synthesis method, the method provided by the invention has the advantages of simple synthesis route, no glycosylation reaction, high reaction yield and good application prospect.

The invention discloses an amino acid sequence and a nucleotide sequence of a heat-resisting alkali-resisting and salt stable inulase exonuclease, and construction and application of an inulase exonuclease recombinant expression vector. Results of enzymatic property research show that the optimum reaction conditions of the inulase exonuclease are as below: pH 7.0, 50 DEG C and 10% NaCl. The enzyme can catalyze the hydrolysis of inulase, levan, sucrose, raffinose, kestose, nystose and Kestopentaose. In a catalysis process using inulase as a substrate, only generation of fructose is detected rather than oligosaccharide or other carbohydrates. The inulase exonuclease provided by the invention can be applied to the field of food industry and biological energy source as a novel biocatalyst for fructose production.

The invention discloses a human blood group antigen P1 pentasaccharide synthesis method. The method includes steps: adopting a one-pot multienzyme system for coupling galactose to trisaccharide as shown in a formula (III) through a beta1-4 glucosidic bond to synthesize tetrasaccharide as shown in a formula (IV); adopting the one-pot multienzyme system for coupling galactose to the tetrasaccharide as shown in the formula (IV) through an alpha1-4 glucosidic bond to synthesize pentasaccharide as shown in a formula (I), wherein in the formula (I), the formula (III) and the formula (IV), R1 refers to hydroxyl, azide substituted alkyl, alkynyl substituted alkyl, sulfydryl substituted alkyl, alpha- or beta- configuration substituted alkyl, alpha- or beta- configuration serine residue and alpha- or beta- configuration threonine residue. By integration of high regioselectivity and high efficiency of enzymatic synthesis, P1 antigen pentasaccharide is synthesized for the first time. Glycosyltransferase, glucose nucleoside generating enzymes and glucokinse adopted in the synthesis method are all derived from prokaryotes, and high protein expression quantity, high substrate adaptability and high catalytic efficiency are realized.

The present invention related to a saccharide-based cholera toxin detection sensor for detection of Vibrio cholerae and its use. More specifically, the present invention relates to a carbohydrate chip for detection of Vibrio cholerae, a method for detecting Vibrio cholerae using the same, and a method for preparing the same, where the carbohydrate chip comprises GM1 pentasaccharide, GM2 tetrasaccharide, asialo GM1 tetrasaccharide, GM3 trisaccharide, galactose-β 1,3-N-acetylgalactosamine, lactose, and sialic acid that are immobilized on the surface of a solid substrate.

The invention relates to a method for detecting target breast milk oligosaccharide in formula food. The target breast milk oligosaccharide may include or be one or more of 2 '-fucosyllactose, 3-fucosyllactose, 3'-sialyllactose, 6 '-sialyllactose, lactose-difucosyltetraose, lactose-N-neotetraose, and lactose-N-tetraose. The method comprises the following steps: removing fat and protein from a sample, reducing by adopting a reducing agent such as NaBH4, detecting by adopting liquid chromatography-mass spectrometry, and quantifying by adopting an external standard method. According to the method, the target breast milk oligosaccharide (HMO) can be simply, conveniently, quickly, accurately, qualitatively and quantitatively detected.

The invention discloses a production method of enoxaparin sodium, which comprises the following steps: carrying out enzymolysis on heparin sodium, carrying out HPLC (high performance liquid chromatography) analysis, calculating the contents of various peaks by a peak area normalization process, determining the varieties of disaccharides and tetrasaccharides of the heparin sodium subjected to enzymolysis according to a standard spectrum of disaccharides and tetrasaccharides after heparin sodium enzymolysis, comparing with the contents of disaccharides and tetrasaccharides of the standard spectrum, and selecting the satisfactory heparin sodium raw material to produce the enoxaparin sodium. The method is simple and effective, can ensure the safety and effectiveness of the product, controls the quality of the enoxaparin sodium from the source, can avoid the waste of the active pharmaceutical ingredient heparin sodium, greatly saves the cost and enhances the production efficiency.