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Preparation method of chondroitin sulfate D tetrasaccharide

A chondroitin sulfate, oligosaccharide technology, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problem of inability to achieve large-scale production, small yield, separation and extraction of chondroitin sulfate specific structure and molecular weight oligosaccharides Methods are not reported, etc.

Inactive Publication Date: 2018-05-25
BINZHOU MEDICAL COLLEGE
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The yield of this method is small, and limited by the yield of preparative electrophoresis, this process cannot be scaled up
[0005] There is no report on the separation and extraction method of chondroitin sulfate specific structure and molecular weight oligosaccharides

Method used

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  • Preparation method of chondroitin sulfate D tetrasaccharide
  • Preparation method of chondroitin sulfate D tetrasaccharide
  • Preparation method of chondroitin sulfate D tetrasaccharide

Examples

Experimental program
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Embodiment 1

[0029] (1) Shark chondroitin sulfate 5g, dissolved in pH8.0 buffer solution at a concentration of 5% (W / V), added 50IU chondroitin sulfate AC enzyme, stirred at 37°C for 48 hours; after the reaction, added 5 times Precipitate with 95% ethanol by volume and freeze-dry to obtain the chondroitin sulfate oligosaccharide mixture.

[0030] (2) Dissolve the sample obtained in step (1) to 0.1g / mL with 0.15mol / L NaCl solution, the chromatographic column size is 2.6×20cm, Q Sepharose Fast Flow strong anion exchange packing, the ultraviolet detection wavelength is 232nm, the injection volume 10ml, mobile phase 0.15mol / L and 1.2mol / L NaCl solution, flow rate 1.33mL / min, gradient elution, collect the elution volume at 1350-1550ml, which is the last peak. See figure 1 .

[0031] (3) The fractions collected in step (2) were desalted by G-25 dextran gel filtration, and then concentrated and freeze-dried.

[0032] (4) Dissolve the lyophilized sample in step (3) into 0.1g / mL with purified wa...

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Abstract

The invention relates to a preparation method of a chondroitin sulfate D tetrasaccharide, and belongs to the technical field of biochemical engineering. According to the preparation method, the chondroitin sulfate D tetrasaccharide is prepared and obtained through steps of ethanol precipitation, anion exchange chromatographic separation, gel chromatographic separation and the like by using shark chondroitin sulfate as a raw material and adopting chondroitin sulfate AC enzyme degradation. The preparation method has the beneficial effects that the chondroitin sulfate D tetrasaccharide with definite structure and molecular weight can be prepared and obtained; the yield is higher, and the probability is provided for the research of a chondroitin sulfate oligosaccharide monomer.

Description

technical field [0001] The invention relates to a preparation method of chondroitin sulfate D tetrasaccharide, which belongs to the technical field of biochemical industry. Background technique [0002] Chondroitin sulfate is a mucopolysaccharide extracted from animal cartilage, which is formed by linking acetylgalactosamine and glucuronic acid. It is used in various fields such as medicine, health care, food, and cosmetics. Chondroitin sulfate has been used in the clinical treatment of neuralgia, arthralgia, arthritis and other diseases. The potential biological activities of chondroitin sulfate include anti-inflammatory, immunomodulatory, neurotrophic and osteoporosis prevention, etc. However, due to its high molecular weight, uneven structure and low cell membrane permeability, natural chondroitin sulfate has problems such as low bioavailability and unstable curative effect. [0003] Low-molecular-weight chondroitin sulfate has been paid more and more attention by peopl...

Claims

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Application Information

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IPC IPC(8): C12P19/04C07H1/06C07H11/00
CPCC12P19/04C07H1/06C07H11/00
Inventor 李燕妮
Owner BINZHOU MEDICAL COLLEGE
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