129 results about "Enzyme degradation" patented technology

A composition comprising in admixture with a polymer, preferably a polyurethane, and a compatible surface-modifying macromolecule having (i) a central portion of a segmented block oligomeric copolymer comprising at least one polar hard segment, and (ii) PROPORTIONAL - omega terminal polyfluoro oligomeric groups, in a surface modifying enhancing amount. The composition is of use in providing articles having improved surface properties, particularly, medical devices having improved resistance to enzyme degradation with acceptable blood compatibility.

The invention relates to a method for breaking walls of cells of bran by wet grinding, and belongs to the technical field of industrial biochemistry. The invention adopts the technical key point that: bran, water, and enzyme for degrading walls of cells of the bran are mixed, and subjected to wet ball-milling grinding to form superfine wall-breaking powder. By combining an ultramicro-pulverization method, the water and the enzyme for degrading the walls of cells of the bran, the cells of the bran are subjected to wet ball milling for wall-breaking treatment; compared with single ultramicro pulverization, the method can greatly reduce the energy consumption, is simple and has low requirement on operating equipment; and compared with single enzymatic degradation, the method combines wet ball milling, combines chemical action with mechanical force action, and has the advantages of greatly saving energy required by high temperature treatment, avoiding the defect of enzyme inactivation under high temperature treatment, eliminating crystal structures of cellulose and improving enzymolysis efficiency.

The invention provides a collagen matrix freezing gel used for biomedical materials and a preparation method thereof. The collagen matrix freezing gel is the collagen extracted from skins or tendons of healthy domestic animals using the enzyme method with the molecular weight of 280 to 320kDa and a well maintained triple helical structure; the collagen reacts for 1 to 7 days in the condition of low temperature of 0 to minus 50 DEG C in a die after through cross linked and modified reaction with hydroformylation polysaccharide, then extrudes the die and is defrosted, thus forming the collagen matrix freezing gel. The preparation of the collagen matrix freezing gel of the invention in particular relates to the extraction of the collagen, the preparation of the hydroformylation polysaccharide and the synthesis of hydroformylation polysaccharide-collagen matrix freezing gel. The hydroformylation polysaccharide-collagen matrix freezing gel prepared by the invention improves the mechanical property, thermal stability and anti-enzyme degradation, etc., of pure collagen gels, and the freezing gel has the advantages of porosity, plasticity, hydrophilic property and non-toxicity, and can be used as biomedical materials such as bio-scaffold, cell cultivation, drug controlled release and biological dressings.

The present invention relates to gelatin preparation, and is especially the enzyme degradation process of preparing gelatin with bone collagen. The process includes milling the mixing of defatted bone material and water into bone slurry with bone size of 1-5 mm; regulating pH value to 1.5-4 with acid or to 7-8 with alkali and adding acid or alkali proteinase in 0.2-0.8 wt% of bone slurry to control the degradation of collagen; reaction at room temperature for 7-10 hr, regulating pH 5.0-6.5 with alkali or acid and heating to 70-85 deg.c for extraction; separating the turbid gelatin obtained via extraction; filtering gelatin liquid to obtain high purity hard capsule gelatin solution. The dried gelatin has molecular weight distribution of alpha=48 % and beta=18 %; gelatin jelly strength over 240 g; viscosity 3.0 mPs; transparency 90 % at 620 nm and 71 % at 450 nm.

The invention relates to a straw bio-enzyme degradation aid in the technical field of straw degradation, which comprises the following components in percentage by weight: 8 to 10 percent of cellulase, 6 to 11 percent of lipase, 6 to 10 percent of alpha-1,4-glucose hydrolase, 4 to 10 percent of catalase, 6 to 20 percent of urea, 6 to 16 percent of magnesium sulfate, 8 to 15 percent of fatty alcohol-polyoxyethylene ether, 11 to 15 percent of alkylphenol polyoxyethylene ether OP and the balance of excipient. A degradation pulping process for the straw bio-enzyme degradation aid comprises the following steps of: cleaning straw, cutting into segments, putting the segments into a quick pulping machine, adding water and the straw bio-enzyme degradation aid, mixing uniformly, and heating to the temperature of between 50 and 60 DEG C in the quick pulping machine for rubbing to perform reaction for 1 to 1.5 hours; and adding hydrogen peroxide for bleaching, and when the whiteness is more than 70 percent which is required by International standard organization (ISO), stopping the reaction to obtain paper pulp. The degradation pulping process is non-pollution, thermophilic digestion is avoided, strong base and strong acid are not used, the requirement of environment-friendly pulping is met, waste water is not discharged during pulping, a large amount of water for production and energy canbe saved, the production cost is reduced, and the pulping speed is accelerated.

The invention is a wool anti-felting finishing method, and its character: the preprocessing adopts chitosan water solution for soaking and adopts neutral proteinase and neutral fatty to make enzyme degradation treatment, thus making the felting ratio of the processed wool fabric less than 3%, and strong damage within 5% and raising balling resistance of wool fiber by 2-3 grades, and also the whiteness, able to completely meet the machine washing requirement. The invention simplifies the processing course and shortens the process flow and has a shorter cycle so as to strengthen the feasibility in industrialized operation and has no residual harmful matters and can not cause environmental pollution.

An extraction method for abelmoschus esculentus juice is disclosed. According to the method, biology enzyme degradation is employed, pectin, starch, aspartame, saccharan and other macromolecules not easily absorbed by human body and in abelmoschus esculentus fruits are subjected hydrolysis, the absorption rate of nutritional compositions in abelmoschus esculentus fruits is improved, and the sensory properties of the abelmoschus esculentus juice product are improved. The obtained abelmoschus esculentus juice is clear in appearance, bright in color and good in mouthfeel, and the abelmoschus esculentus juice processed by employing the method has the saccharan content higher than the abelmoschus esculentus juice processed by employing a conventional method by 56%.

The invention discloses a potato slag comprehensive utilization processing method for combined production of starch and meal fiber. Mechanical crushing and enzyme degradation method are used for processing potato slag, so as to significantly improve the processing properties of materials, and effectively promote the release of target and generation of high active components. At the same time, medium recycling utilization process is utilized, so as to not only save water consumption but also simplify the subsequent concentration process, and effectively realize enrichment of target object. The obtained starch product has good transparency, swelling power and retrogradation property compared with other starch; and the obtained dietary fiber product has high proportion of soluble dietary fiber, strong sodium cholate, and especially obvious advantages in the aspects of adsorption ability of cholesterol adsorption compared with similar products, which shows that the dietary fiber product has good physical property and physiological activity. The invention has the advantages of simple operation, convenience, mild conditions, low equipment requirement, high utilization rate of raw materials, energy saving and benefit for environmental protection.

There are provided a novel series of peptide analogs of hGH-RH(1-29)NH2 and hGH-RH(1-30)NH2 which show high activities in stimulating the release of pituitary GH in animals. They retain their physiological activity in solution for extended periods of time and resist enzymic degradation in the body. These novel and useful properties appear to be due to novel substitution patterns ant at the 1, 15, 27 and 29 positions on the peptide.

The invention relates to a preparation method of a chondroitin sulfate D tetrasaccharide, and belongs to the technical field of biochemical engineering. According to the preparation method, the chondroitin sulfate D tetrasaccharide is prepared and obtained through steps of ethanol precipitation, anion exchange chromatographic separation, gel chromatographic separation and the like by using shark chondroitin sulfate as a raw material and adopting chondroitin sulfate AC enzyme degradation. The preparation method has the beneficial effects that the chondroitin sulfate D tetrasaccharide with definite structure and molecular weight can be prepared and obtained; the yield is higher, and the probability is provided for the research of a chondroitin sulfate oligosaccharide monomer.

Disclosed is a method for preparing of an egg perfume compound with a biological enzyme preparation, using yolks as raw material, hydrolyzing with enzymolysis technique, comprising the following steps: substrate concentration adjustment by adding water, hyperthermia denaturation, cooling down, enzymes degradation, enzymes inactivation, centrifugal separation, vacuum concentration, emulsification, and maillard reaction. The method is characterized in that complex enzyme preparation, which is a hybrid of proteinase and lipase, is applied in enzymolysis art; the complex enzyme preparetion is added in a quantity of 1%-10% by wt of the stock; the enzymolysis temperature is 35-60 DEG C, and the enzymmolysis time is 2-6 hours; the enzymes inactivation temperation is 85-100 DEG C, and the enzymes inactivation time is 10-30 minutes. Compared with traditional arts, the method disclosed avoids stock multiformity or waiting for new stock appearance. The egg perfume compound has a fresh, strong, and real fragrance of cooked eggs, and is an ideal natural food additive.

The invention discloses a method for preparing functional xyio-oligosacchandes by taking agricultural wastes as raw materials and using ultrasonic treatment and bio-enzyme degradation. The method comprises the following steps: hydrolyzing the agricultural wastes after the ultrasonic treatment by using endo xylanase generated by Streptomyces rameus, converting hemicellulose in the agricultural wastes into the high-quality xyio-oligosacchandes, and preparing a xyio-oligosacchandes product having low xylose content and the polymerization degree between 2 and 5.

The invention relates to a method for high-yield production of oat polysaccharide with naked oat bran as a raw material, which adopts the naked oat bran as a raw material and comprises the following steps: super-micro pulverization, ethanol pre-treatment, extraction for a plurality of times with water as extraction solvent, mixing of extracts, addition of enzyme degradation starch, isoelectric point deproteinization or deproteinization through hydrolysis with proteolytic enzyme, vacuum concentration, ethanol precipitation, static keeping, filtration, sediment collection and freeze drying or hot air drying, thereby obtaining the oat polysaccharide. The extraction rate of the oat polysaccharide in the invention is higher than 12g of oat polysaccharide per 100g of naked oat bran, the monosaccharide components of the oat polysaccharide comprise xylose and glucose, and the ratio of (1-3) glucosidic bonds and (1-4) glucosidic bonds is 1:1.3-1.3:1.

The invention relates to a method of improving tobacco quality by sprinkling industrial protease to tobacco leaves to degrade protein in tobacco leaves; meanwhile the influences caused by temperature, humidity, enzyme addition and enzyme-degradation time, etc. to enzymatic degradation to protein in tobacco raw materials are observed. The results show that the invention can effectively reduce protein content in tobacco and increase amino acid content; smoking evaluation results show that the tobacco has a good smell, a nice mouth feeling and a greatly improved quality.

A coupled enzymolysis method for preparing chitooligose product includes such steps as dissolving chitopolyose while stirring, biological enzyme degradation, pretreating, enzyme degradation again, pretreating again, ultrafiltering for separating high-bioactivity chitooligose solution, and nano-filtering. Its apparatus is composed of dissolving-enzyme degradating-filtering unit, secondary enzyme degradating-filtering unit, ultrafilter separator, and nano-filter. Its advantages are low cost and high output rate.

The invention discloses a preservative for fetus free DNA in peripheral blood of pregnant woman and a vacuum blood sampling tube composed of the preservative. The problems of short storage time and easiness in degrading of the fetus free DNA in plasma caused by the shortage of mature collecting and storing technique for the in vitro test of the fetus free DNA in peripheral blood of pregnant woman are solved. The preservative provided by the invention comprises a cell stabilizing agent, an anti-coagulant agent, a buffering solution, a metabolic inhibitor and a DNA enzyme inhibitor. The preservative can stabilize the karyocyte of the peripheral blood of pregnant woman, can prevent the cell chromosome DNA from being released, can restrain DNA enzyme degradation and is beneficial to the stability of fetus free DNA.

The invention discloses a preparation and application of a nano-composite system of a polycation macromolecular prodrug used for gene targeted transfer. The composite system comprises a high polymer material, such as polycation, a targeted group, an antitumor drug and a target gene. According to the preparation and application of nano-composite system disclosed by the invention, the advantages ofnanometer drug delivery system and macromolecular prodrug are integrated; the antitumor drug and the target gene are coupled to polycation through chemical bonds so as to compound the macromolecular prodrug, and then a self-assembling method is adopted for preparing a macromolecular prodrug nano-composite system; the target gene is coated during a self-assembling process, the target gene is effectively protected and the gene is prevented from enzymatic degradation. According to the preparation and application of nano-composite system disclosed by the invention, the polycation macromolecular prodrug serves as a gene vector, co-transfer of drug and gene is realized, advantages of gene therapy and chemotherapy are integrated and the nano-composite system has an excellent application prospectin the aspect of oncotherapy.

The invention discloses a comprehensive aquatic product processing leftover utilization method which comprises the following steps: with processing leftovers of crustacean including fish, shrimp, crab and the like as the raw materials, cleaning, low-temperature drying, cryogenic grinding, complex enzymatic degradation, organic acid extraction, high-speed centrifugal separation, reverse osmosis dehydration, molecule embedding, high-pressure homogenizing, vacuum low-temperature cryogenic drying, pelletizing, tabletting, automatic bottled packaging production line processing and outer packing are carried out, and all high value-added bio-active substances including astaxanthin, protein, organic calcium, chitin and the derivative of the chitin namely chitosan in the aquatic product processing leftovers are extracted, so that resources are comprehensively and effectively utilized without waste discharge. According to the invention, the moderate bio-enzyme degradation technique is adopted, the whole process is carried out under the low-temperature condition, strong acid, strong base and toxic solvents are not used, the technological process is short, the industrial chain is long, and the products are high in bioactivity, various in variety, excellent in quality, safe to eat, wide in application fields, large in potential market, and high in comprehensive economic benefit.

The present invention related to providing a novel medicine for treating diabetes. It is possible to provide a GLP-1 derivative which is resistant to enzyme degradation by glycosylation of the peptide side chain.

The invention provides a method for extracting active ingredients of fresh pilos antler through enzymatic degradation. The fresh pilos antler is treated through Alcalase alkali protease degradation, the biologic active substance concentrated solution of the pilos antler is obtained, and the degradation rate is 82 percent to 93 percent; the obtained biologic active substance concentrated solution of the pilos antler contains various active ingredients such as fatty acid, phospholipids, steroid, glucide, trace elements and the like, all organic substances become a solution state easy for human body to absorb, and the active ingredients can be used for various aspects such as pharmacy, health care products, food, cosmetics and the like; the Alcalase alkali protease is used for degrading the fresh pilos antler, and residues after fresh pilos antler degradation are pilos antler calcification materials, inorganic elements and the like and can be used as good feed additives; after the separation and purification of the biologic active substance concentrated solution of the pilos antler, the obtained amino acid products with the purity being 97 percent are used as important nourishments, and the amino acid yield reaches more than 12 percent. The method has the advantages that the reaction condition is mild, the operation is simple, all the active ingredients of the pilos antler can be fully utilized, and the application value and the economic benefit of the pilos antler are improved.

Disclosed are polypeptide compounds containing at least one residue comprising a cyclically-constrained gamma-amino acid residue. The compounds have the formula where A is a hydrogen, hydroxy, amino- or carboxy-protecting group, Y is a single bond or a prolyl-containing linking group, and X and Y are gamma-amino acid residues, provided that one of X or Y is a conformationally-restrained gamma-amino acid residue, and "a," "c," and "d" are positive integers. The compounds find use as non-enzymatically degradable probes to mimic protein behavior in solution.

The invention discloses a method for separating heparan sulfate from animal lungs, belonging to the technical field of biological pharmacy. The method comprises the following steps: by using animal lungs as the raw material, carrying out composite enzyme degradation on an animal lung brine extract by using hydrolases, adding an oxidizer and activated carbon into the enzymolysis solution to perform oxidative adsorption decolorization, carrying out fractional precipitation on the oxidized solution by using acetone, dehydrating to obtain a heparan sulfate crude product, sequentially carrying out membrane separation and anion exchange chromatography on the crude product heparan sulfate water solution to separate the heparan sulfate from dermatan sulfate, chondroitin sulfate, heparin and other impurities, carrying out ultrafiltration concentration on the eluate through a 5000-7000Da ultrafiltration membrane, desalting by gel filtration chromatography, and freeze-drying to obtain the heparan sulfate refined product. The method has advantages of simple technological conditions, accessible raw materials and low cost, and is easy to implement; and the obtained heparan sulfate has high purity.

The invention discloses a process for preparing low molecular weight chitosan through degrading compound enzyme. The synergistic effect which is produced in the process of degrading chitosan by various non-specific enzymes of alpha-amylase and bromelain or cellulose is utilized to increase the degrading efficiency, which enables the average molecular weight of products to degrade and reach about 1500 Da in 5.0 hours. After the final degradation products are microfiltration and nanofiltration purified, concentrated, and spray dried, the low molecular weight chitosan product with high quality can be obtained. Compared with a traditional process for preparation, the process not only has high product activity, good quality and convenient operation but also improves the manufacturing environment, which is beneficial to realize industrialization.

T The invention discloses amino-terminated hyperbranched polyamide modified waste leather scraps used as a graphene oxide single-lamella small-size dispersing agent and a preparation method and application of the amino-terminated hyperbranched polyamide modified waste leather scraps. According to the invention, the amino-terminated hyperbranched polyamide modified waste leather scraps and carboxymethylated modified graphene oxide are subjected to intercalation compounding so as to prepare a graphene oxide nanosheet dispersion liquid which has few lamellas and a small size and is suitable for being used as a leather tanning agent and a fatliquoring agent. According to the method disclosed by the invention, a leather scrap degradation solution with a relatively small relative molecular massis prepared by controlling alkali degradation and enzyme degradation conditions of the waste leather scrap; intercalation dispersion of the graphite oxide nanosheet layer is facilitated; the formationof steric hindrance and repulsive force between sheet layers is facilitated; graphene oxide nanosheet layers are relatively high in hydrophilicity and dispersity in water, and the prepared graphene oxide single-lamella small-size dispersion liquid is used for tanning and fatliquoring leather, so that the softness, fullness and elasticity of the leather can be obviously improved, and the dischargeamount of a chrome tanning agent, a dye and a fatliquoring agent in wastewater is reduced.

The invention provides a method for preparing an aloe lyophilized powder containing macromolecular substance, firstly, the fresh aloe gel is obtained by cleaning, disinfecting and peeling the fresh leaf, rinsing the aloe gel to obtain the glutinous mucus, heating and filtering, decoloring, filtering and concentrating the aloe juice, rinsing the aloe fiber and extracting by alcohol to obtain the dry fiber, adding the dry fiber into the concentrated liquid, lyophilizing and crushing to obtain the lyophilized powder. Without using enzyme degradation, the invention can decrease the barbaloin content, the viscosity of the mucus, maintaining the macromolecular substance, so as to exert the wound recovery and scald removal functions, the yield rate of the product is high, the production cost is low and the extraction process is simple, it can be used for batch production of aloe gel lyophilized product with high quality.

Disclosed is a process for preparing oligo xylose product adopting high temperature degradation process according to structural feature and thermal degradation mechanism of xylan. The process is characterized in that the optimum reaction temperature used in high temperature degradation is 180í‚2íµ, and the optimum reaction time is 30í‚5min. By comparison to the oligo xylose preparing process by enzyme degradation, the inventive process flow is shortened greatly, and the reaction process is quickened obviously.

Low molecular weight low molecular weight chitosan oligomers were able to self-assemble siRNA into nanosized particles, provide protection against enzymatic degradation, and mediate gene silencing that is stable over a long period of time in vitro. The control of structural variables in formulating complexes of siRNA with low molecular weight chitosans provides an efficient alternative delivery system for siRNA in vitro and in vivo.

Said invention refers to a pharmaceutical form for selective colon delivery of drugs or bioactive molecules degraded and / or poorly absorbed in the gastrointestinal tract. The system comprises a core consisting of the active ingredient, and a protease inhibitor layer and / or an absorption enhancer layer, said core being separated from these layers by means of a polymer that swells and / or dissolves and / or is degraded when in contact with the biological fluids present in the gastro-intestinal tract; depending on the thickness of the polymeric layer, the release of the drug can be modulated with respect to the inhibitor and / or promoter.

The invention discloses a kitchen lampblack five-section comprehensive treatment system. An intelligent deodorizing device is connected with an intelligent purifying device through a lampblack pipe; the intelligent purifying device is connected with an exhaust device through a lampblack pipe; the exhaust device is provided with an exhaust port; an intelligent cleaning device is mounted at the intelligent purifying device; an intelligent detecting device is arranged in the lampblack between the intelligent purifying device and the exhaust device; the intelligent detecting device comprises a wind speed measuring probe, a non-methane hydrocarbon probe and a lampblack concentration detecting probe; the intelligent deodorizing device, the intelligent purifying device, the intelligent cleaning device and the intelligent detecting device are connected with an intelligent monitoring device; and the intelligent monitoring device is in wireless connection to a cloud platform. The kitchen lampblack five-section comprehensive treatment system has the advantages of treatment of kitchen lampblack by adopting VOCS oxidation decomposition intelligent deodorization, three-section ESP electrostaticintelligent purification and biological enzyme degradation intelligent cleaning, intelligent prewarning by adopting real-time detection and monitoring, convenience for data information inquiry and comprehensive supervision.