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Method of preparing chitin oligosaccharide by coupled enzymolysis and its equipment

A technology of chitosan oligosaccharides and enzyme coupling, applied in the direction of fermentation, etc., can solve the problems of lower equipment utilization, clogging of membrane separation reactors, and large index fluctuations, so as to improve utilization, increase yield, and reduce clogging problem effect

Inactive Publication Date: 2003-04-16
YUFENG BIOLOGICAL ENG HUBEI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because these methods have problems such as complex preparation process, large index fluctuation, serious environmental pollution, low product yield, high preparation cost, and inconsistent physiological activity of the product, the industrialized large-scale production of chitosan oligosaccharide products is limited.
[0004] The method and device for preparing chitooligosaccharides by using conventional membrane separation bioreactor technology for enzymatic hydrolysis of chitosan, due to the high requirements for raw materials, in the process of industrial scale production, a relatively high input cost is required. Because of the secondary treatment of raw materials, and in the follow-up process, because the ash and undegraded macromolecular substances brought in with the raw materials are not separated, the membrane separation reactor will be seriously blocked, and the utilization rate of the equipment will be reduced, so that in the production Cost, production cycle, production equipment investment, environmental pollution reduction and product yield have affected industrial scale production

Method used

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  • Method of preparing chitin oligosaccharide by coupled enzymolysis and its equipment
  • Method of preparing chitin oligosaccharide by coupled enzymolysis and its equipment
  • Method of preparing chitin oligosaccharide by coupled enzymolysis and its equipment

Examples

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Effect test

Embodiment 1

[0037] Example 1: Select hemicellulose complex enzyme as the chitosan degrading enzyme, the best environment for enzymatic degradation is: pH=5.3, temperature t=47°C, enzyme concentration 0.1%, substrate concentration 10%; according to the above Technical parameters, add 1000L (liter) of pure water into the dissolution-enzyme degradation reaction kettle 1, then add a certain amount of acetic acid, adjust the pH value of the solution in the kettle to 5.3, and the temperature of the reaction liquid in the kettle is at 47°C±1°C , then add 100kg chitosan, stir to dissolve, after 1 hour, check the pH value of the feed liquid in the kettle, if there is any change, you need to add the corresponding acid or alkali to adjust, after passing the test, disperse 1000mL of the prepared biological enzyme Add it into the kettle, and under the condition of maintaining the temperature of the reaction solution in the kettle, stir and degrade it. After 2 hours, the viscosity of the reaction soluti...

Embodiment 2

[0042] Embodiment 2: adopt the equipment condition of embodiment 1, select for use papain, pectinase, cellulase and lysozyme to be mixed with a certain amount of compound enzyme by a certain proportion, through testing, obtain the hydrolysis optimal environment of this compound enzyme The parameters are: pH=4.5, temperature t=42°C; the enzyme concentration in the reaction solution is 2%, and the substrate concentration is 3-4.5%;

[0043] According to the technical parameters required above, add 1000L of pure water into the dissolution-enzyme degradation reaction kettle 1, and then add a certain amount of acetic acid to adjust the pH value of the solution in the kettle to 4.5, and the temperature of the reaction liquid in the kettle is 42 ° C ± 1 ℃, add 30kg of chitosan, stir to dissolve, and prepare a reaction solution with a substrate concentration of 3% and a pH of 4.5. After 1 to 2 hours, check the pH value of the feed liquid in the kettle. If there is any change, you need...

Embodiment 3

[0046] Example 3: Using the equipment conditions of Example 1, this example selects hemicellulose complex enzyme as the chitosan degrading enzyme, and the best environment for enzymatic degradation is: pH=5.3, temperature t=45°C; The enzyme concentration is 0.2%, and the substrate concentration prepared according to the amount of material is 5%;

[0047] According to the technical parameters required above, add 1000L of pure water into the dissolution-enzyme degradation reaction kettle 1, and then add a certain amount of acetic acid to adjust the pH value of the solution in the kettle to 5.3, and the temperature of the reaction liquid in the kettle is 45 ° C ± 1 ℃, add 50kg of chitosan, stir to dissolve, and prepare a reaction solution with a substrate concentration of 5% and a pH of 5.3. After 0.5 hours, check the pH value of the feed liquid in the kettle. If there is any change, you need to add the corresponding acid or alkali to adjust it. Under the condition of the liquid...

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Abstract

A coupled enzymolysis method for preparing chitooligose product includes such steps as dissolving chitopolyose while stirring, biological enzyme degradation, pretreating, enzyme degradation again, pretreating again, ultrafiltering for separating high-bioactivity chitooligose solution, and nano-filtering. Its apparatus is composed of dissolving-enzyme degradating-filtering unit, secondary enzyme degradating-filtering unit, ultrafilter separator, and nano-filter. Its advantages are low cost and high output rate.

Description

technical field [0001] The invention relates to a method and equipment for industrial scale production of chitosan oligosaccharide products with different polymerization degrees and high activity by applying multi-stage pretreatment and membrane separation bioreactor coupled enzymatic hydrolysis of chitosan. Background technique [0002] Chitooligosaccharides are chitooligosaccharides with 4 to 20 glucosamines polymerized in β(1,4) glycosidic bonds by reducing the degree of polymerization of chitosan. Due to the excellent physiological affinity of chitosan oligosaccharides, it is widely used in: 1. The development of medicine and new drugs, especially in the development of new drugs for enhancing human immunity and treating cancer; 2. It has obvious Effective health care products and health food additives and daily cosmetics; 3. Biological and crop growth regulators and pollution-free biological pesticides; 4. It has been used as a textile coating agent or woven into fibers,...

Claims

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Application Information

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IPC IPC(8): C12P19/04
Inventor 程飚王学峰曾庆本高晋安
Owner YUFENG BIOLOGICAL ENG HUBEI
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