Process for preparing gelatin from enzyme degradation bone collagen

Abstract

The present invention relates to gelatin preparation, and is especially the enzyme degradation process of preparing gelatin with bone collagen. The process includes milling the mixing of defatted bone material and water into bone slurry with bone size of 1-5 mm; regulating pH value to 1.5-4 with acid or to 7-8 with alkali and adding acid or alkali proteinase in 0.2-0.8 wt% of bone slurry to control the degradation of collagen; reaction at room temperature for 7-10 hr, regulating pH 5.0-6.5 with alkali or acid and heating to 70-85 deg.c for extraction; separating the turbid gelatin obtained via extraction; filtering gelatin liquid to obtain high purity hard capsule gelatin solution. The dried gelatin has molecular weight distribution of alpha=48 % and beta=18 %; gelatin jelly strength over 240 g; viscosity 3.0 mPs; transparency 90 % at 620 nm and 71 % at 450 nm.

Description

technical field [0001] The invention belongs to the field of gelatin preparation, and in particular relates to a method for preparing gelatin by enzymatically degrading bone collagen to produce high-purity, high-grade gelatin suitable for producing hard capsules. Background technique [0002] Gelatin is a protein extracted from the connective tissue (skin and bone) of animals (cattle, horse, pig) through a multi-step long degradation. The gelatin industry has a history of more than 100 years, and has been extracting bone gelatin from bone collagen by using the traditional alkaline process. The biggest advantage of the traditional alkaline process to prepare gelatin is: simple operation, separate product quality grades, the highest quality photographic glue can be obtained, and a certain proportion of industrial glue can also be produced. However, there are always three major insurmountable defects in the preparation of gelatin by the traditional alkali...

AI Technical Summary

Problems solved by technology

However, there are always three major insurmountable defects in the preparation of gelatin by the traditional alkaline process: first, the production cycle is long, ranging from 60 to 100 days depending on the season, climate and region

The great disadvantage brought by the long production cycle is that the production efficiency is extremely low, especially the quality of gelatin produced between different batches cannot be guaranteed to be unchanged

Second, the water consumption is large, 1 / 2 of the 18 processes require water, especially the three major steps of washing, pickling and liming require a lot of water

Third, the pollution of the environment is serious, and a large amount of waste acid and waste liming water are discharged nearby, causing serious environmental pollution in surrounding areas

A large number of literature surveys show that, so far, these developed countries have not been able to produce pharmaceutical gelatin with new technology.