398 results about "Bone collagen" patented technology

The invention discloses a preparation method of yak bone collagen peptide. The method is characterized by comprising the following technological processes: pretreatment of raw materials, gelatin extraction, separation of a collagen extracting solution, composite enzymatic hydrolysis, enzyme deactivation and sterilization, separation of a peptide solution, decoloring and deodorizing, concentrating and drying. The compound enzyme for composite enzymatic hydrolysis is prepared from protease, amylase, pectinase, cellulose and xylanase. The method has the advantages that the gelatin extraction process is simple, oil and gelatin are completely separated by using flow of a mixed solution, the enzymatic hydrolysis temperature is 48-52 DEG C, the time is 4-6 hours, the temperature is reduced in comparison with common enzymatic hydrolysis temperature, the preparation time of the product is shortened, and the method comprises enzyme deactivation and sterilization procedures, and decoloring and deodorizing procedures, so that the product is good in taste, and applicable to preparation of the yak bone collagen peptide.

The invention discloses a moistening and repairing facial mask (named meitianfu (Chinese character) moistening and repairing facial mask, and hereinafter called moistening and repairing facial mask) and a preparation method thereof. The moistening and repairing facial mask disclosed by the invention contains the following components: water, butanediol, sodium hyaluronate, carbomer, triethanolamine, carboxymethyl beta-sodium glucosan, a witchhazel extract, a tremella fuciformis extract, an arabian jasmine flower, a german chamomile extract, deer bone collagen protein, a rosemary extract, a scoparia dulcis extract, asiaticoside, a silk extract, a sargassum fusiforme setch extract, acetyl tetrapeptide-9, palmitoyl tripeptide-5, pentapeptide-3, acetyl hexapeptide-8, phenoxyethanol and ethylhexylglycerin. The facial mask prepared by the method disclosed by the invention has the characteristics of being safe, effective and free of irritation.

The present invention relates to gelatin preparation, and is especially the enzyme degradation process of preparing gelatin with bone collagen. The process includes milling the mixing of defatted bone material and water into bone slurry with bone size of 1-5 mm; regulating pH value to 1.5-4 with acid or to 7-8 with alkali and adding acid or alkali proteinase in 0.2-0.8 wt% of bone slurry to control the degradation of collagen; reaction at room temperature for 7-10 hr, regulating pH 5.0-6.5 with alkali or acid and heating to 70-85 deg.c for extraction; separating the turbid gelatin obtained via extraction; filtering gelatin liquid to obtain high purity hard capsule gelatin solution. The dried gelatin has molecular weight distribution of alpha=48 % and beta=18 %; gelatin jelly strength over 240 g; viscosity 3.0 mPs; transparency 90 % at 620 nm and 71 % at 450 nm.

The invention relates to a method for extracting bone collagen protein from bone of pig, cow and sheep, including: It removes the impurities of the bone. And then it warms up and extracts the bone under high-pressure in the high-pressure extraction tank. And then it separates the oil and melting soup by using oil-water separator. And then it transfers the separated liquid into the enzymolysis can for enzymatic hydrolysis. And then it cools the liquid to 50 deg.C, and then adds liquid complex flavor protease to enzymolysis for eight hours. And then it warms the liquid up to 90 deg.C, and it eliminate enzyme for 30 minutes. And then it adds natural spice and salt. And then it filters the liquid. And then it sterilizes the liquid with a high-temperature sterilization machine. And then it condenses the liquid to dry materials by using double-effect falling film evaporator. And then it dries the dry materials into power by using pressure-type sprayer.

The invention relates to the technical field of food, and discloses a hot pot base and a preparation method thereof. In the hot pot base, a bovine bone soup powder, bovine bone marrow powder and a beef extract which are rich in bone collagen, various amino acids such as hydroxyproline and the like, vitamins and various trace elements such as calcium, phosphorus, ferrum and the like are added into beef tallow, glutinous rice cake hot pepper, ginger, garlic, broad bean paste, fermented soya beans, table salt, white sugar, spice and monosodium glutamate which serve as raw materials, so that the hot pot base has the abundant nutrition while having fresh and fragrant taste. Compared with the hot pot base in the prior art, the hot pot base has the characteristic of fresh and fragrant taste and nutrition. The hot pot base prepared by the method is peppery but not dry, pungent but not bitter and fresh but not thin, is greased and mellow, has moderate mouthfeel and abundant nutrition, and is suitable to be eaten by people which have different dietary habits in every place.

The invention relates to a novel calcium supplementing preparation with functions of improving bones and joints, which is prepared from the following raw materials in parts by weight: 10-42 parts of calcium source components, 5-25 parts of amino sugar components, 0.1-5 parts of casein phosphopeptide, 0.5-5 parts of bone collagen protein, 0-8 parts of oxidation resistant components for human bodies and 0-3 parts of filling agents. The novel calcium supplementing preparation not only can supplement a calcium source to increase the bone hardness, but also can supplement bone collagen protein to increase the bone toughness for preventing calcium loss; the casein phosphopeptide is added to promote the calcium absorption, thereby solving the problem of the calcium absorption in the calcium supplementing process; and simultaneously, the oxidation resistant components and the components for promoting osteoblast proliferation are added to enable the bone reconstruction process formed by bone formation and bone absorption to balance again, thus the supplemented calcium can be reserved and firmly deposited on the bones. The related components in the novel calcium supplementing preparation can restore cartilages, improve the joint structure and interdict the deterioration of osteoarthritis.

The invention relates to a method for preparing low-molecular-weight sheep bone collagen polypeptide calcium chelate microcapsules, which comprises the following steps of: 1, preparing sheep bone meal, namely grinding fresh sheep bones, degreasing, drying, and grinding to prepare sheep bone paste; 2, performing enzymolysis on the sheep bone paste to prepare bone collagen polypeptides; 3, separating and extracting by using an ultrafiltration membrane to obtain low-molecular-weight bone collagen polypeptides; 4, chelating the low-molecular-weight bone collagen polypeptides and a calcium source to prepare low-molecular-weight collagen polypeptide calcium chelates; and 5, preparing the low-molecular-weight sheep bone collagen polypeptide calcium chelate microcapsules. By the method, the addedvalue of sheep bone resources is improved, the comprehensive development and utilization of collagen and bone calcium in pig bones is realized, and the prepared microcapsule calcium supplement has high solubility in water, and has the highest solubility in a range close to human body temperature; and the microcapsule product can cover the peculiar smell of calcium salt.

The invention discloses a preparation technology of yak bone collagen oligopeptide. The preparation technology comprises the following steps: grinding yak bones, which have been dried in the shade, into powder with a size of 50 meshes, processing yak bone powder by alkali and acid, removing fat and other impurities, extracting yak bone powder to obtain high purity yak bone collagen liquid extract; subjecting the yak bone collagen liquid extract to two-step enzymatic hydrolysis, after enzymatic hydrolysis, purifying the enzymatic hydrolysate by a centrifuge under the assistance of a multi-stage membrane separation system so as to obtain a yak bone collagen oligopeptide solution; spray-drying the yak bone collagen oligopeptide solution to obtain yak bone collagen oligopeptide; wherein the purity of yak bone collagen oligopeptide is more than 90%, and the molecular weight of yak bone collagen oligopeptide is less than 1000 Da. The yak processing byproduct namely yak bone is utilized to prepare collagen oligopeptide, the cost is low, the collagen extraction rate is more than 85%, the impurity removing effect and condensing effect are good, and the method is suitable for massive industrial production.

The invention discloses a fishbone soup product and a preparation method thereof. The fishbone soup product is prepared by the steps of: selecting aquatic product leftovers such as fish head, and fish steak as raw materials, and preparing delicious nutritious fishbone soup product after crushing and stewing the raw materials and sterilization of the soup. The fishbone soup is rich in protein, phosphatide and calcium, has delicate taste but no fishy smell basically, and can be used as seasoning to produce products of various special flavors according to the requirements of eating and cooking. By crushing raw materials as fish head and fish steak into granular fish bones before making fishbone soup, bone collagen, unsaturated fatty acid, protein, trace elements as calcium and the like are better dissolved in the process of soup making, and time and energy needed in stewing are saved. The preparation method of fishbone soup product is advantaged in that: fish leftovers can be fully utilized, and waste of resource is reduced.

The invention relates to a preparation method for a zinc chelated collagen peptide of tuna fishbone collagen. The preparation method specifically comprises the following steps: using tuna fishbone as a raw material; adopting an acid process to extract collagen; performing enzymolysis on the collagens with pepsin and trypsin; adopting ultrafiltration, macroporous resin column chromatography, gel column chromatography and reversed-phase high-performance liquid chromatography to separate and purify the collagens to obtain the zinc chelated collagen peptide Gly-Lys-Thr-Gly-Trp-Pro-Gly (GKTGWPG), wherein the molecular weight of the zinc chelated collagen peptide detected by ESI-MS is 701.73 Da. The zinc chelated collagen peptide is high in absorption and utilization due to the unique chelating and transport mechanism, and meanwhile capable of supplementing polypeptides / amino acid and zinc, so that the zinc chelated collagen peptide is an ideal zinc-supplementing substance.

The invention relates to the field of medical food and discloses an aquatic collagen peptide food used for adjuvant therapy of osteoporosis and arthritis. Every 100g of the food contains multiple nutritional ingredients as follows: 9-11g of cod skin collagen protein peptide powder, 4-6g of squid skin collagen protein peptide powder, 2-3g of whey protein powder, 2-3g of soybean protein powder, 9-11g of MCT fat, 2-3g of soybean oil, 58-62g of carbohydrate and other vitamins and microelements. The aquatic collagen peptide food takes cod skin collagen protein peptide powder, squid skin collagen protein peptide powder and whey protein powder as main raw materials and has relatively high digestive absorptivity, and collagen protein peptide can promote bone formation and reinforce a bone collagen structure at low calcium level, so that bone strength is improved; bone metabolism can be improved, and ossification can be enhanced; and bone diseases such as the osteoporosis, hyperostosis and swelling and pain of bone joints can be prevented.

The present invention relates to a method for inducing the bionic calcification in collagen fibers through a polymer polyelectrolyte, and applications thereof. The method comprises: treating different types of collagen scaffolds in a calcium phosphate mineralized solution containing a cationic polyelectrolyte or anionic polyelectrolyte to obtain the bionic calcification collagen scaffold material having the inside-fiber mineralization characteristic, wherein the collagen scaffold is recombinant collagens type I having different sources, completely demineralized bone collagen, completely demineralized dentin collagen, or rat tail collagen, and the mineralization liquid is a calcium phosphate solution added with a cationic polyelectrolyte or anionic polyelectrolyte. According to the present invention, through the stabilizing effect of the polyelectrolyte on the calcium phosphate solution, the novel bionic calcified collagen scaffold material with characteristics of similar natural bone tissue structure, good mechanical property, good biocompatibility and suitable biodegradability is constructed.

The invention provides collagen composite peptide powder. The collagen composite peptide powder is characterized by comprising the following components in following ratios: 93.40%-96.00% of yak bone collagen peptide, 1.00%-3.00% of nitrariadites, 0.14%-0.16% of taurine, 0.20%-0.26% of vitamin C, 0.11%-0.18% of anhydrous citric acid, 0.40%-0.60% of vanilla flavor, 0.03%-0.04% of orange flavor, 2.00%-2.10% of a bitter taste removing agent, 0.02%-0.06% of acesulfame and 0.10%-0.20% of milk flavor. A preparation method comprises the following steps of: (1) carrying out quality inspection, wherein main and auxiliary materials need to meet a product quality standard; (2) weighing: accurately weighing the weights of the main and auxiliary materials; (3) mixing: adding the main and auxiliary materials into a mixer according to a formula ratio and agitating uniformly; (4) sieving: sieving a mixture by a 40-mesh vibration sieve and filtering; (5) filling: filling the mixture into a bag by using a packaging machine; (6) packing into a box: packing the powder into the box; adding a drying agent and sealing the opening; and (7) checking a finished product and storing the qualified finished product. The collagen composite peptide powder provided by the invention has the advantages that the collagen composite peptide powder is easily absorbed by a human body and can be used for rapidly relieving fatigues.

The invention relates to a tuna bone collagen peptide preparation method. The tuna bone collagen peptide preparation method includes: crushing tuna bones, subjecting the crushed tuna bones to pretreatment, adding additives to prepare collagen and performing enzymolysis to obtain a tuna bone collagen peptide. The preparation method is short in preparation cycle, high in yield and product purity and capable of removing bitter fishy smell, provides technical assistance for development of selenium-supplementing health food and drugs and also provides a new thought for high-value application of the tuna bones, and the tuna bone collagen peptide is good in taste and beneficial to absorption of human bodies.

The invention relates to a preparation method of a selenium-chelated collagen peptide with tuna bone collagen serving as a source. The preparation method includes: crushing tuna bones, subjecting the crushed tuna bones to pretreatment prior to enzymolysis and adding selenium-enriched yeast so as to obtain the selenium-chelated collagen peptide. The preparation method is short in preparation cycle, high in yield and product purity and capable of removing bitter fishy smell, provides technical assistance for development of selenium-supplementing health food and drugs and also provides a new thought for high-value application of the tuna bones, and the selenium-chelated collagen peptide is good in taste and beneficial to absorption of human bodies.

The invention discloses a donkey meat flavor instant noodle seasoning bag, which consists of a meat paste bag, a vegetable auxiliary material bag and a seasoning auxiliary material bag, wherein the meat paste bag mainly comprises donkey meat, edible oil and fresh soup; the vegetable auxiliary material bag mainly comprises green Chinese onion, carrot and mushroom; and the seasoning auxiliary material bag mainly comprises gourmet powder and table salt. The seasoning bag effectively considers the nutrition and flavor of donkey meat, has high protein, low fat, a large amount of amino acid and low cholesterol and has good health-care effects on arteriosclerosis, coronary disease and hypertension; and the seasoning bag also comprises ingredients such as animal glue, bone collagen, calcium nitrate and the like, so that the seasoning bag can replenish high nutrition for the aged, children, weak people and people in recovery after disease.

The invention relates to a medicinal composition for strengthening bones, which is prepared from the following raw materials in part by weight: 35 to 50 parts of calcium citrate, 35 to 55 parts of glucosamine, 5 to 10 weight parts of eucommia bark extract, 1 to 3 parts of soy isoflavones, 1 to 5 parts of chondroitin sulfate and 0.001 to 0.008 part of vitamin D. The medicinal composition is based on a strict formula and has a special and remarkable effect; the raw materials of the medicinal composition are widely available; and the cost of the medicinal composition is low. The simplicity of the formula makes medicament quality control simple. The components are combined to produce a synergetic effect for tonifying liver and kidney, supplementing calcium, strengthening bones and relieving pain in bone joints and consequently for increasing bone density, preventing and treating osteoporosis, promoting synthesis of bone collagen and proteoglycan, retaining damaged joints and bones and strengthening muscles and bones. The medicinal composition increases bone density, prevents osteoporosis and relieves pain in bone joints by exerting influences on the systems and organs of the body and regulating the links and factors associated with bone and joint metabolism.

The invention discloses a deer collagen nutritional composition. The composition comprises the following components in percentage by weight: 70-95 percent of deer collagen, 1-10 percent of glucosamine, 1-10 percent of hyaluronic acid, 0.1-5 percent of soy isoflavone, and 0.1-6 percent of tea polyphenol, wherein the deer collagen comprises deer sinew collagen, deer bone collagen, deer skin collagen in a weight ratio of 1:1:1; the molecular weight of the deer sinew is distributed below 1,500 daltons. The deer collagen serving as a main component is assisted by glucosamine, hyaluronic acid, soy isoflavone and tea polyphenol with oxidation resistance to form the nutritional composition capable of strengthening bone density and remarkably improving osteoporosis.

The invention discloses an extraction method of collagen polypeptide and active calcium in walleye pollock bone. The method comprises the following steps: (I) pretreating the raw material; (II) preparing bone collagen polypeptide through the substeps of (1) soaking fish bone in a NaOH solution and then carrying out high-temperature and high-pressure treatment, (2) extracting the bone collagen, (3) carrying out treatment via a high-voltage pulsed electric field, and (4) carrying out enzymolysis by firstly adding composite collagen protease for enzymolysis, then adding flavor protease for enzymolysis, decolorizing and deordorizing, carrying out spray drying to obtain the collagen polypeptide; and (III) preparing active calcium in fish bone through the substeps of (1) extracting tartaric acid, (2) extracting tartaric acid again, and (3) combining the extracted tartaric acids to prepare the active calcium in the fish bone. Through the extraction method, the disadvantages of thick fishy smell, bitter taste and unfavorable peculiar smells of the marine source collagen polypeptide can be overcome; the collagen polypeptide of the walleye pollock bone which is free of pungent smell, delicious and moderate in taste can be prepared; meanwhile, the bone dreg can be economically and efficiently used for preparing active tartaric acid chelated calcium; the utilization rate of the walleye pollock bone is increased; the high-value utilization is achieved.

The invention belongs to the field of health care food, and especially relates to a composition used for improving skeletal system health, and applications thereof. The composition comprises skimmed milk powder, fructo-oligosaccharide, yak bone, orange fruit powder, D-glucosamine hydrochloride, bone collagen, chondroitin sulfate, herba epimedii extract, rhizoma drynariae extract, mushroom powder, magnesium carbonate, casein phosphopeptide, and vitamin K2. The composition possesses excellent prevention effects on patients with osteoporosis and skeletal system disorders such as ankylosis, arthritis, osteoproliferation, arthralgia, and myospasm, and is capable of increasing bone mineral density, and improving skeletal system health. The composition can be used for preparing health care food capable of improving skeletal system health; application range is wide; and economic benefit and social benefit are both high.

The invention relates to a method for preparing a pig bone collagen polypeptide chelated calcium supplement. The method comprises the following steps of: 1, preparing pig bone paste, namely crushing, defatting and grinding fresh pig bones to obtain the pig bone paste; 2, preparing bone collagen polypeptide, namely preparing a pig bone paste culture solution, inoculating mixed strains of serratia marcescens and lactobacillus bulgaricus, fermenting, centrifuging, filtering and drying to obtain the bone collagen polypeptide; 3, preparing soluble bone calcium by mixing bone paste residue left after fermentation and hydrochloric acid; and 4, preparing the bone collagen polypeptide chelated calcium supplement, namely chelating the bone collagen polypeptide and the soluble bone calcium to obtainthe bone collagen polypeptide chelated calcium supplement. By the method, the added value of a pig bone resource is improved, the collagen and bone calcium in the pig bones are comprehensively developed and utilized, and the prepared chelated calcium supplement has high solubility in water, is favorable for a human body to absorb and utilize calcium element in the chelate, and can be applied to various fields such as chemical engineering, foods, cosmetics and the like.

The technological process for extracting compound bone collagen from animal soft bone includes the following steps: cleaning, hydrolyzing by using basic aqueous solution, cooling and adding acid, filtering, concentrating, alcohol precipitation, dewatering, drying and packaging so as to obtain the invented product which is low in fat content, high in collagen protein content and contains trace elements of calcium, magnesium and potassium, etc and 19 amino acids. The chondrine content is up to abouve 20%, and the content of harmful substances of lead, arsenic and mercury is less than National Food Safely Standard. Said product can be used for producing several health-care foods, and can be added in various foods, and can be used in domestic chemical industry.

The invention relates to tuna bone collagen peptide capable of promoting bone cell growth and a preparation method thereof. The method comprises the steps of decalcification treatment, compound enzyme fish bone degreasing enzymolysis and purification and drying; for the tuna bone collagen peptide obtained through the method, the molecular weight is 500-2,000 Da, the contact angle is 10.0-25.0 degrees, and MC3T3-E1 cell growth can be promoted under the concentration of 5-200 microgram / mL. Accordingly, the preparation technology is scientific and reasonable, fish bones which are not utilized in aquatic product processing are fully utilized as raw materials, collagen peptide with potential market value is developed, compound enzyme degreasing enzymolysis method is adopted, the compound enzyme enzymolysis condition is adjusted, and the tuna bone collagen peptide which is capable of promoting the bone cell growth, safe, free of toxic and side effects and the like is obtained. The production efficiency is improved, energy consumption is lowered, the technology is scientific and reasonable, and operation is easy.

The invention provides a composition for preventing and treating bone arthritis. The composition is characterized by comprising non-denaturing type II collagen and cartilage collagen peptide accordingto a weight ratio of 1:(0.5 to 30), preferably 1:(1 to 20), more preferably 1:(1.5 to 10), and most preferably 1:(1.5 to 6). The invention also provides application of the composition in preparationof a product for preventing and treating the bone arthritis, wherein the product is a medicine or health-care food. The composition for preventing and treating bone arthritis has the advantages that the cartilage collagen peptide can be used as a raw material needed for synthesizing cartilage tissues, the degradation of joints is prevented, and the repair function is realized on the injured joints; the non-denaturing type II collagen can inhibit the generation of inflammation, so as to relieve and treat the arthritis; proved by experiment results, the combination of the cartilage collagen peptide and the non-denaturing type II collagen can achieve a synergistic effect on the preventing and treatment of arthritis.

The invention provides a health care food used for increasing bone mineral density, preventing osteoarthrosis and enhancing immunity. The health care food is prepared from the following materials in parts by mass: 1500-2500 parts of marine fish bone collagen oligopeptide, 1500-2500 parts of fish bone powder, 200-400 parts of eucommia bark extract and 0.005-0.01 part of vitamin D and a proper amount of pharmaceutic adjuvants such as xylitol, dry skim milk, vegetable fat powder, essences, trichlorosucrose and the like. The health care food is prepared into a granular preparation by the following process steps of: premixing, granulating, total mixing and the like. The invention has the beneficial effects that the raw materials, i.e., marine fish bone collagen oligopeptide and fish bone whichcontain a large quantity of peptide, bone collagen and calcium, work together with eucommia bark extract and vitamin D in the health care food so that a human body can absorb effective ingredients better to increase bone mineral density, slow down bone marrow aging, enhance toughness of bones, and prevent and treat osteoporosis; in addition, peptide also has the functions of adjusting immunity and resisting bacteria and oxidation, thereby improving the immunity of the human body.

The invention relates to eel bone collagen drinking liquor and a production method thereof. The method comprises the following steps: performing enzymolysis on eel bone collagen to prepare eel bone collagen extract; extracting hawthorn fruit, lemon and the like by using water; and finally mixing and stirring the components uniformly according to proportion to prepare a finished product. The eel bone collagen drinking liquor is a pure natural biological product; and the whole production process is simple and quick, completely keeps the active nutrient components of the eel bone collagen, and does not bring other harmful substances at the same time. The amino acid composition of the eel bone collagen is superior to that of other animal collagen. The molecular weight of the collagen peptide prepared by enzymolysis and hydroxylation of the advanced production process is about 3,000 Daltons, and the collagen peptide is the most ideal choice for human body absorption and collagen utilization. The eel bone collagen drinking liquor contains high-content characteristic collagen amino acid, and has comprehensive and balanced nourishing effect by reasonably adding the auxiliary materials such as the hawthorn fruit, the lemon and the like.

The invention provides a calcium supplementing preparation. The calcium supplementing preparation comprises the following raw materials in parts by weight: 20-30 parts of bone collagen, 6-10 parts of milk minerals, 0.8-1.2 parts of casein phosphopeptides, 0.4-0.6 part of colostrum basic protein, 0.0000067-0.00001 part of vitamin D3, 26-40 parts of maltodextrin, 21-33 parts of skimmed milk powder and 4-6 parts of sweet orange powder. A preparation method of the calcium supplementing preparation is simple in preparation process and has good effects. The calcium supplementing preparation is used for scientifically supplementing calcium aiming at each stage at which skeletons require comprehensive nutrients. A scheme of supplementing, absorbing, conveying, locking and surviving is adopted to enable the skeletons to actively absorb calcium, promote absorption of calcium, also implant calcium on the skeletons to lock calcium, stimulate osteoblast, suppress the activity of osteoclast, promote skeleton rebuilding and surviving calcium.

The invention relates to a method for preparing a dual-factor carrying type hybrid bionic bone scaffold and application of the dual-factor carrying type hybrid bionic bone scaffold. The preparation method comprises the following steps: immersing a calcium- silicon hybrid bionic bone scaffold in fibers in a chemotactic factor-osteogenesis inducing protein mixed solution with proper concentration; treating under a proper condition to obtain the dual-factor carrying type hybrid bionic bone scaffold. The related application is application of the scaffold material in a bone defect repairing material. The hybrid ossein contains silicon dioxide and hydroxyapatite two minerals, wherein due to the effective combination of massive negative charge-loaded silanol groups existing on the surface of the silicon dioxide and active hydroxyl radicals on basic protein such as SDF-1 or TGF-beta and the specifically strong adsorptivity of nano-hydroxyapatite on osteogenesis induced protein, such as BMP-2 or BMP-7, an extra sustained-release system need not to be added, and hybrid osteocyte recruitment / osteogenesis induction double functionalized modification can be realized.

The invention discloses a preparation method of three-marrow powder, which comprises the following steps: 1. extracting effective ingredients such as compound phosphatide of brain marrows, bone marrows and spinal cords of animals by using an extraction technology, and then preparing the effective ingredients into a three-marrow extract powder by using a low-temperature freeze drying technology; 2. carrying out biological enzymolysis on animal bones to extract effective ingredients such as bone collagen protein polypeptide, and preparing the effective ingredients such as bone collagen protein polypeptide into animal bone extract powder by using a high-pressure spray method; 3. after the animal bones subjected to enzymolysis are dried, preparing the animal bones into animal full-bone nutrition powder by using a superfine grinding technology; and 4. after the three-marrow extract powder, the animal bone extract powder and the animal full-bone nutrition powder are mixed in certain proportion, supplementing vegetable origin food to the obtained mixture so as to prepare various series of three-marrow function foods through scientific matching. Produced three-marrow powder can economically and effectively improve the physiological function of a human body, and can enhance the immunity, improve the bone mineral density and reduce the bone mineral loss.

The invention discloses a formula for supplementing nutrients of bone and improving functions of bone and a preparation method. The formula comprises the following components in percentage by mass: 30to 70% of bone collagen protein peptide powder, 1 to 10% of L-aspartic acid calcium, 1 to 10% of hydrolyzed II-type collagen, 1 to 10% of casein phosphopeptide powder, 1 to 10% of milk minerals, and0.0002 to 0.005% of vitamin D. The formula has the advantages that according to the cause of bone diseases, several kinds of pure animals and plants are used as the raw materials and are reasonably compounded and scientifically proportioned, so that while the content of calcium in a human body is supplemented, the bone collagen protein is supplemented, and the full absorbing of calcium is accelerated; after the formula is taken for a long time, the demand and intake amount of calcium by the human body can be adjusted, and the formula is especially suitable for patients with osteoporosis or arthritis; any side or toxic effect is avoided, the auxiliary treatment effect is realized on treatment of various bone diseases, the nutrients of the bone can be supplemented, the functions of the bonecan be improved, and the functions of enhancing the immunity of the human body, resisting the aging and resisting the fatigue are realized.