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Preparation technology of yak bone collagen oligopeptide

A preparation process and collagen technology are applied in the field of preparation technology of yak bone collagen oligopeptide, which can solve the problems of difficult control of product quality stability and the inability to achieve uniform molecular weight of collagen oligopeptide, and achieves good impurity removal and concentration effects, low cost effect

Inactive Publication Date: 2016-03-09
QINGDAO ARCTIC YAK BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the "one-step enzymatic hydrolysis method" adopted by this method is difficult to concentrate the molecular weight of the hydrolyzed peptide in the range of 100 to 1000 Da, and there is no membrane purification step in the later stage of production, so the molecular weight of the collagen oligopeptide in the product cannot be uniform, and the quality of the product is low. Stability is hard to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. In the embodiment of the present invention, pre-treatment: use high-quality yak bones as raw materials, which can be marine yak bones, or freshwater yak-like yak bones. Wash 5kg of yak bones dried in the shade with water, crush them to 50 mesh, put them into the reaction kettle, add 30L of sodium hydroxide aqueous solution with a concentration of 0.002kg / L, stir for 2 hours, wash with water until neutral; then add 30L of Aqueous hydrochloric acid solution with a concentration of 0.6% was stirred for 1 hour, washed with water until neutral; finally, 5 L of water was added, and heated at 60° C. for 4 hours to obtain a yak bone collagen extract.

[0027] 2. Enzymolysis: Add 0.05kg of trypsin to the above-mentioned yak bone collagen extract to carry out the first step of enzymolysis. The enzymolysis temperature is 40°C, and the pH is adjusted to 7.0 with sodium hydroxide solution, and the enzymolysis is carried out for 6 hours. After enzymatic hydrolysis, raise the tempe...

Embodiment 2

[0031] 1. Pre-treatment: wash 5kg of yak bones dried in the shade with water, crush them to 50 mesh, put them into the reaction kettle, add 30L of sodium bicarbonate aqueous solution with a concentration of 0.01kg / L, stir for 2 hours, and wash with water until neutral Then add 30L of hydrochloric acid aqueous solution with a volume concentration of 0.8%, stir for 1 hour, wash with water until neutral; finally add 5L of water, heat at 60° C. for 4 hours to obtain a yak bone collagen extract.

[0032] 2. Enzymolysis: Add 0.1kg of alkaline protease to the above-mentioned yak bone collagen extract to carry out the first step of enzymolysis, the enzymolysis temperature is 50°C, adjust the pH to 6.5 with sodium hydroxide solution, and enzymolysis for 4 hours After the enzymolysis, the temperature was raised to 85°C, and the enzyme was inactivated at a constant temperature for 15 minutes to obtain an enzymolysis solution; then 0.2kg of acidic protease was added to the enzymolysis solu...

Embodiment 3

[0036]1. Pretreatment: Wash 5kg of dried yak bone with water, crush it to 50 mesh, put it into the reaction kettle, add 40L of sodium hydroxide aqueous solution with a concentration of 0.004kg / L, stir for 2 hours, and wash with water until neutral Then add 40L of hydrochloric acid aqueous solution with a volume concentration of 1%, stir for 1 hour, wash with water until neutral; finally add 5L of water, heat at 60°C for 2 hours to obtain yak bone collagen extract.

[0037] 2. Enzymolysis: Add 0.5 kg of trypsin and 0.5 kg of papain to the above-mentioned yak bone collagen extract to carry out the first step of enzymolysis, the enzymolysis temperature is 50°C, and the pH is adjusted to 7.5 with sodium hydroxide solution , enzymatic hydrolysis for 10 hours, after enzymatic hydrolysis, the temperature was raised to 80°C, and the enzyme was inactivated at a constant temperature for 20 minutes to obtain a primary enzymatic hydrolysis solution; then 1kg of acidic protease was added to...

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Abstract

The invention discloses a preparation technology of yak bone collagen oligopeptide. The preparation technology comprises the following steps: grinding yak bones, which have been dried in the shade, into powder with a size of 50 meshes, processing yak bone powder by alkali and acid, removing fat and other impurities, extracting yak bone powder to obtain high purity yak bone collagen liquid extract; subjecting the yak bone collagen liquid extract to two-step enzymatic hydrolysis, after enzymatic hydrolysis, purifying the enzymatic hydrolysate by a centrifuge under the assistance of a multi-stage membrane separation system so as to obtain a yak bone collagen oligopeptide solution; spray-drying the yak bone collagen oligopeptide solution to obtain yak bone collagen oligopeptide; wherein the purity of yak bone collagen oligopeptide is more than 90%, and the molecular weight of yak bone collagen oligopeptide is less than 1000 Da. The yak processing byproduct namely yak bone is utilized to prepare collagen oligopeptide, the cost is low, the collagen extraction rate is more than 85%, the impurity removing effect and condensing effect are good, and the method is suitable for massive industrial production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation process of yak bone collagen oligopeptide. Background technique [0002] Yak bone is a by-product of yak processing, and its protein content is very high, especially the content of collagen accounts for more than half of its total protein. The small molecule collagen oligopeptide obtained by hydrolyzing collagen with protease has unique physiological activities, such as: promoting skin collagen metabolism, preventing osteoporosis and improving immunity, etc., and can be widely used in many fields such as cosmetics, medicine and functional food. The resources of yak bone in the Qinghai-Tibet Plateau are abundant and the price is low. Considering the cost of raw materials, the use of yak bone to prepare collagen oligopeptides has more application prospects and value. [0003] In recent years, as the application value of collagen oligopeptides has attracted more and more ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 许卫红
Owner QINGDAO ARCTIC YAK BIOLOGICAL TECH CO LTD
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