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Method for high-yield production of oat polysaccharide with naked oat bran as raw material

A technology for oat polysaccharide and naked oat is applied in the field of obtaining high yield oat polysaccharide, and can solve the problems of insufficient extraction of oat polysaccharide, difficulty in extraction, low yield and the like

Inactive Publication Date: 2010-08-11
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0007] Because oat polysaccharides are mostly combined with proteins, there are difficulties in extraction. The existing extraction methods cannot fully extract oat polysaccharides, and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment

[0025] 1. Weigh 1000kg of naked oat bran, and use an ultrafine pulverizer to pulverize it to make it finer than 200 mesh.

[0026] 2. Add 50%-80% (V / V) ethanol aqueous solution to the above-mentioned processed naked oat bran, and the ratio of material to liquid is 1: (3-4) (g naked oat bran / v ethanol solution). Incubate at 60-80°C for 10-40min, discard the supernatant, and repeat the previous operation 2-3 times for the insoluble matter in the lower layer.

[0027] 3. Ethanol-treated naked oat bran, volatilize to remove ethanol, add distilled water, the material / liquid ratio is (6-10): 1 (mL distilled water: g naked oat bran), keep warm at 70°C-90°C for 80min- After 120 min, filter and collect the filtrate, repeat the above operation twice for insoluble matter, combine the filtrates, and concentrate in vacuo to one-third of the original volume.

[0028] 4. Add 10000-60000U of heat-resistant amylase to the filtrate, heat to above 95°C, keep warm for 10-30min, cool to 60°C, add...

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Abstract

The invention relates to a method for high-yield production of oat polysaccharide with naked oat bran as a raw material, which adopts the naked oat bran as a raw material and comprises the following steps: super-micro pulverization, ethanol pre-treatment, extraction for a plurality of times with water as extraction solvent, mixing of extracts, addition of enzyme degradation starch, isoelectric point deproteinization or deproteinization through hydrolysis with proteolytic enzyme, vacuum concentration, ethanol precipitation, static keeping, filtration, sediment collection and freeze drying or hot air drying, thereby obtaining the oat polysaccharide. The extraction rate of the oat polysaccharide in the invention is higher than 12g of oat polysaccharide per 100g of naked oat bran, the monosaccharide components of the oat polysaccharide comprise xylose and glucose, and the ratio of (1-3) glucosidic bonds and (1-4) glucosidic bonds is 1:1.3-1.3:1.

Description

technical field [0001] The invention belongs to the technical field of oat deep processing, in particular to a method for obtaining high-yield oat polysaccharides from naked oat bran. Background technique [0002] Dietary fiber is a natural organic polymer compound, which is a non-starch polysaccharide formed by the dehydration polymerization of monosaccharides. It cannot be decomposed by digestive enzymes in the human body, but can be degraded and utilized by microorganisms in the large intestine. An indispensable class of carbohydrates for health. [0003] Previous studies believed that the main component of oat polysaccharides was β-glucan, and its molecular structure was a linear polysaccharide composed of D-glucose connected by β-(1,3) and β-(1,4) glycosidic bonds. The ratio of glycosidic bonds is roughly 7:3; the distribution of β-(1,3) and β-(1,4) glycosidic bonds in oat β-glucan is neither completely ordered nor completely disordered, and β- The (1,3) bond exists a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 张民梁漪白鑫
Owner TIANJIN UNIV OF SCI & TECH
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